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Offlineillegalsmilerk
Stranger
Registered: 08/19/02
Posts: 14
Last seen: 13 years, 11 months
Oven Tek
    #1019094 - 11/03/02 08:44 PM (14 years, 1 month ago)

I plan on trying the oven tek for innoc and transfers but was unsure on details of the tek...Should I worry about my kitchen being sterile, also What temp should I heat the oven too and how long should i let warm for, 400 Degrees sound good?


Any Response will be appreciated plan on doing this tonight or tomarrow morning


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OfflineStS
treetoker
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Registered: 08/01/00
Posts: 1,821
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Re: Oven Tek [Re: illegalsmilerk]
    #1019112 - 11/03/02 08:51 PM (14 years, 1 month ago)

Just set it at the lowest oven setting (about 150-200). Let it warm up for about 10 minutes. The idea is that the hot air (which is more sterile than the room air) is what's flowing over your work area (heat rises) thereby reducing the chance of airborne contaminants. Hope this helps.


--------------------

"You took too much, man....too much, too much!"


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OfflineTheHobbit
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Registered: 09/04/02
Posts: 863
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Re: Oven Tek [Re: illegalsmilerk]
    #1019122 - 11/03/02 08:56 PM (14 years, 1 month ago)

Here's what I do:

Clean the floor area within at least a few feet of the oven with Mr. Clean disinfectant floor cleaner
Disinfect the surface ares where I'll be keeping things I'll be using, such as my upside down shot glass to put denatured alcohol (a couple drops) on for sterilizing the syringe, etc.
Take a shower and put on clean clothes
Lighting the oven to 300 is fine
I use a dust mask, and put on latex gloves too - I'll have washed my hands and face with disinfectant soap
Open the oven, pull out a rack to put the jars on
Put the jar you're doing on the rack, take off the tape, sterilize the syringe, shake the syringe good, inoculate:)


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OfflineTheHobbit
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Registered: 09/04/02
Posts: 863
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Re: Oven Tek [Re: TheHobbit]
    #1019126 - 11/03/02 08:57 PM (14 years, 1 month ago)

AND LYSOL!!!!!! Use generously in the air around your oven just prior to working.


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OfflineDERRAYLD
Constructus

Registered: 05/13/02
Posts: 1,712
Loc: South Africa
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Re: Oven Tek [Re: TheHobbit]
    #1019705 - 11/04/02 12:59 AM (14 years, 1 month ago)

Hobbit all those precautions are totally unnecessary.
The point of the oven tek is that air borne contamination is lifted away from the clean area.
So by cleaning everything first, sure you`re maintaining a clean area but at the same time none of those contaminations your clening off will make it into your jars anyway.

I generally just preheat my oven for maybe 1min and then starts working, making sure I rub my hands lebirally with Alcohol and maintain slow movements.


--------------------
Derrayld and out of control


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OfflineSensiFool
Newbie
Registered: 09/30/02
Posts: 33
Last seen: 13 years, 11 months
Re: Oven Tek [Re: illegalsmilerk]
    #1019732 - 11/04/02 01:10 AM (14 years, 1 month ago)

The heat doesn't kill the spores?


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InvisibleFick_Duck
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Registered: 10/18/02
Posts: 209
Loc: The Hamburger Train
Re: Oven Tek [Re: SensiFool]
    #1019813 - 11/04/02 01:53 AM (14 years, 1 month ago)

D is right, ive gotten really sloppy with the oven tek lately cause it works like frieckin magic! i never lysol anything just alcohol your tools and greasy little fingers and youll have no problem.


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"To know life you must fuck it in the liver." -Dr. Frankenstein, Andy Warhols Frankenstein


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Offlinekykeon
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Registered: 05/30/02
Posts: 1,506
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Re: Oven Tek [Re: DERRAYLD]
    #1019851 - 11/04/02 02:17 AM (14 years, 1 month ago)

DERRAYLD seems to be out of control but i am afraid he is right.

he does various jobs in front of the oven :smile: :laugh: lol lol

Kykeon
athens, greece 


--------------------
The living ghost of Kykeon


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Offlinesocratesmind
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Registered: 02/22/02
Posts: 1,193
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Re: Oven Tek [Re: kykeon]
    #1019915 - 11/04/02 03:12 AM (14 years, 1 month ago)

Hell i know a guy who does open air in a bathroom with lysol and wipes surfaces with 10% bleach solution and flame sterilizes tools praying not for a flash fire and he gets no contams and doesnt have to battle the heat over the oven :laugh:


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Prohibition will work great injury to the cause of temperance. It is a species of intemperance within itself, for it goes beyond the bounds of reason in that it attempts to control a man's appetite by legislation, and makes a crime out of things that are not crimes. A Prohibition law strikes a blow at the very principles upon which our government was founded.
- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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OfflineStS
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Re: Oven Tek [Re: socratesmind]
    #1019937 - 11/04/02 03:22 AM (14 years, 1 month ago)

For over a year now, I don't even bother spraying lysol or anything.  The only precautions I take are washing my hands, wearing a bandana as a mask, and flame sterilizing the needle between each jar.  Believe it or not, 0 contams in that time!  A lot of people do have a way overkill, but if it makes them feel better, by all means they should keep with their practices. :grin:


--------------------

"You took too much, man....too much, too much!"


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Invisiblerazer
enthusiast
Registered: 10/07/01
Posts: 316
Re: Oven Tek [Re: StS]
    #1020234 - 11/04/02 08:43 AM (14 years, 1 month ago)



--------------------
All events stated by myself are only fictionous stories, any relations to true events are intirely coincidental.


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OfflineDERRAYLD
Constructus

Registered: 05/13/02
Posts: 1,712
Loc: South Africa
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Re: Oven Tek [Re: razer]
    #1020272 - 11/04/02 09:07 AM (14 years, 1 month ago)

Read it fine, believe it NO.
I was using the bathroom sprayed down, it worked fine but I needed a better alternative without spending tons.
So I spoke to a more experienced cultivator than myself and he suggested the oven.
I decided to give it a wirl.
I opened 6 petris with agar on each and transferred isolates and spores to each of the petris.
Noting that all 6 were left open in the oven.
And I can say that all the petris survived without even a slight bit of contamination.
Also I haven`t had any contamination in months.
So you choose, I`ll stick with the oven.


--------------------
Derrayld and out of control


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OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
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Re: Oven Tek [Re: DERRAYLD]
    #1020296 - 11/04/02 09:18 AM (14 years, 1 month ago)

Hmmmm, I could be going a little overboard, but as a newb I should probably err on the side of caution. I think I may be contam free so far with my initial effort (small black slightly elongated spots on one jar, which has since been overwhelmed by the mycelia, and expanded ever so slightly initially before staying the same). Not really alot of work, really, just some cleaning. I have a bunch of pets in my house too, so maybe the more anal the better.


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Invisiblerazer
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Registered: 10/07/01
Posts: 316
Re: Oven Tek [Re: TheHobbit]
    #1020802 - 11/04/02 01:46 PM (14 years, 1 month ago)

Well, I believe it, from experience. Did a few attempts with it for innoculating Karo, and they all contamed.


--------------------
All events stated by myself are only fictionous stories, any relations to true events are intirely coincidental.


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Offlinescifi5001
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Re: Oven Tek [Re: razer]
    #1020836 - 11/04/02 02:00 PM (14 years, 1 month ago)

The oven tek would work better if you first :
Heat up the oven (400F) for 30-60min.
Take it down to 200F.

Then carry on as the post that you referred to say.

Heres the post (for the lazy ones):
Quote:


By ratdog (Ratdog) on Wednesday, July 31, 2002 - 05:46 pm: Edit


I have been doing my karo jars using the oven tek, as long as you use common sense sterility procedures, you shouldnt have a problem.

1. Open oven door, pull out rack.
2. Turn oven on to 250F-300F.
3. Wait untill hot air is pouring out of the oven.
4. Completely wipe your entire sterilized karo jar with IPA.
5. Remove the ring/band from the top, leave the lid, gently slide the lid over a few mm, then stick in the needle and innoc/suck.
6. Slide lid back into place
7. Replace band

I've had 0contams using this method, but then again your milage may vary.





I have a jar of colonised agar which i have opened up 4 times and scraped the surface (substitute for agar wedges). Always using the (modified) oven tek.
Point is that it still is ok!  :laugh: 


--------------------
Nothing lasts, nothing is lost.


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Amazon Shop for: ½ Pint Jars, Agar, Petri Dish

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