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Offlinenew2you
Stranger
Registered: 11/04/02
Posts: 5
Last seen: 20 years, 23 days
uneven pinset problems
    #1019061 - 11/04/02 03:27 AM (20 years, 28 days ago)

I got these casings, no overlay so why the uneven pin set? what are the causes? the casing depth is relaive to the substrate, the substrate was fully colonised prior to casing.


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OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
Loc: the Oily Way...
Last seen: 19 years, 1 month
Re: uneven pinset problems [Re: new2you]
    #1019143 - 11/04/02 04:05 AM (20 years, 28 days ago)

Did you patch with casing in spots that came up before much of the rest did? I'm new but have read that you should watch for the first appearence of mycelia on top of the casing and spot patch to help even the pin set out.


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Invisibleda_Vinci7
Stranger
Registered: 03/18/02
Posts: 22
Re: uneven pinset problems [Re: new2you]
    #1019240 - 11/04/02 04:48 AM (20 years, 28 days ago)

If you started with a multi-spore innoculation in the beginning, you essentially created many different strains at once, some of these will fruit better/faster than others and some will not be able to fruit at all. So no matter how well you manage the casing layer/pin initiation phase, you may not get an even pinset sometimes due to the fact there many strains competing against each other. Almost without fail you will have some grow faster than others, while some parts of the casing will grow none at all.(Sometimes this may be attributed to lack of nutrients/air exchange/moisture and not the strain itself). With all things being equal, to achieve the pinset your probably talking about , you will need to learn cloning or sectoring out strains on agar. If I'm wrong about any of this, somebody please correct me as I hate to give out bad info :confused: :grin: 


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Offlinenew2you
Stranger
Registered: 11/04/02
Posts: 5
Last seen: 20 years, 23 days
Re: uneven pinset problems [Re: da_Vinci7]
    #1019463 - 11/04/02 06:19 AM (20 years, 28 days ago)

mulitspore? can you clarify what this is, thanks!



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Offlinenew2you
Stranger
Registered: 11/04/02
Posts: 5
Last seen: 20 years, 23 days
Re: uneven pinset problems [Re: TheHobbit]
    #1019545 - 11/04/02 06:54 AM (20 years, 28 days ago)

yeah i patched (sprinkled) some casing mix on the mycelium that started poking though the casing. i don;t know if it was a good idea though cause overlay is about dead myclium on top of the casing, the mycelium i patched was fresh and alive.


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InvisibleFick_Duck
Truffle Shuffle
Male

Registered: 10/19/02
Posts: 247
Loc: The Hamburger Train
Re: uneven pinset problems [Re: new2you]
    #1019842 - 11/04/02 09:11 AM (20 years, 27 days ago)

multispore inoculation is like from a print or a syringe, which prduces many strains, as opposed to an isolated strain or section of mycelium.


--------------------
"To know life you must fuck it in the liver." -Dr. Frankenstein, Andy Warhols Frankenstein


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OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/14/02
Posts: 6,745
Loc: South Africa
Last seen: 1 hour, 15 minutes
Re: uneven pinset problems [Re: Fick_Duck]
    #1019979 - 11/04/02 11:04 AM (20 years, 27 days ago)

not strains.
Multispore produces many competeing genomes which can fight for dominance of the nutrients.


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Offlinenew2you
Stranger
Registered: 11/04/02
Posts: 5
Last seen: 20 years, 23 days
Re: uneven pinset problems [Re: DERRAYLD]
    #1021596 - 11/05/02 12:38 AM (20 years, 27 days ago)

so basically a multispore is the the same strain of a certain cubensis. and syringes are all multispores?


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Offlinecheifdog24
enthusiast

Registered: 10/22/02
Posts: 270
Last seen: 19 years, 4 months
Re: uneven pinset problems [Re: new2you]
    #1021609 - 11/05/02 12:41 AM (20 years, 27 days ago)

There are substrains in a strain. For instance, if you are working with B+, there are short-fat B+ and tall skinny B+, and so on and so forth.


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OfflineDERRAYLD
Constructus
Male User Gallery

Registered: 05/14/02
Posts: 6,745
Loc: South Africa
Last seen: 1 hour, 15 minutes
Re: uneven pinset problems [Re: cheifdog24]
    #1022833 - 11/05/02 08:41 AM (20 years, 26 days ago)

Those aren`t substrains cheifdog, don`t fight with me.
Those are merely genetic differences in tha particular strain resulting from a multispore innoculation.
By isolating specific characteristics you can maintain a fat mushroom or an even flusher with many fruit, or a flusher that produces big fruit but only a few big fruit.
By using a multispore innoculation these characteristics can occure many times on one casing.
That`s why we isolate on agar, to get a stronger culture with one or a few stronger characteristics and also to minimise the chances of genetic variances.
Kind of like cloning with marajuana.
You take cuttings of a mother plant and clone the strongest characteristics.
As long as you can keep the mother growing, the longe you will grow clones that have similar if not identical characteristics.

Mushroom isolating isn`t a precise art like Herb cloning but the chances of you getting good growth and good fruiting characteristcs from a strong isolate are way bigger than a multispore innoculation.


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