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Registered: 07/05/08
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Re: Artificial introduction of sugars into plants for increased growth rate [Re: LitCloset]
    #10187178 - 04/17/09 09:08 PM (14 years, 10 months ago)

LitCloset: The agar just serves to support the plant as it is growing, you can do liquid tissue cultures [more complex and typically consisting of isolated plant cells in solution] or use liquid agarless media + cotton/foam raft/whatever but you need something to keep the plant material from 'drowning'. I have grown cultures without agar using cotton and rafts before but I prefer agar. As for lighting I have put some of my cultures under a 1000W HPS light and it didn't seem to negatively affect them, but it is totally superfluous, all the sugars the plant needs are in the media, more light wont make them grow any faster! I was actually thinking about your potential contamination problems and not wanting to work in a sterile environment all the time etc. There is a product called PPM [Plant Preservative Mixture], I can't post a link because of the forum rules [as far as I know] but it's easy to find on google. It's designed for tissue culture, and is really great stuff, it prevents the growth of bacteria and fungi without interfering with the plant growth. Adding that to your injections would take care of any contamination problems [if you have them - it's been my experience that all sorts of nasty stuff grows like crazy in 3% sugar solution!].


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