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ashesofman
Pulvis et umbra sumus



Registered: 03/18/09
Posts: 142
Loc: Norway
Last seen: 11 years, 20 days
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Red metabolites? EDIT: Pictures added
#10151772 - 04/12/09 12:18 PM (14 years, 10 months ago) |
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My GT jars are around 95% colonized. On two of the jars there are very red spots, maybe 1-1.4 millimeters in diameter. They are tiny, and blood red. There does not seem to be any residual liquid being produced, or and bleeding of color into the mycelium.
With the first jar, I noticed a ~1.4mm red spot in the mycelium which was branching off into the dry vermiculite layer. I isolated the jar, and ths far I have noticed no growth, or additional spots.
With the second jar, I noticed two red spots, 1mm and 1.25mm respectively, on the mycellium which was advancing upon the bottom of the jar. These again are a deep red, reminiscent of blood. They are maybe 5mm apart, and these too have not grown. There is also a yellowish discoloration on the mycelium, which I assume is definitely metabolites. This jar I did not isolate, for two reasons. One is that the holes are covered in micropore tape, and two is that I really suspect these to be metabolites.
A third jar has a tiny reddish spot, but it is too small to tell if it isn't just vermiculite. I will try to post pictures later, however this is going to be quite the feat with my camera. My zoom is terrible, and I have no lens.
Okay, so the real question is, has anyone experienced anything like this in their jars before? Can metabolites be red?
Below are picture of the jars in question:
Jar one - 2x mag

Jar one - 4x mag

Jar one - inoculation hole

Jar two - 2x mag

Jar two - 4x mag

Jar two - 2.5x mag

Jar two - 4x mag

Jar three - 2x mag

Jar three - 4x mag (blurry, sorry)

Jar four - Wet spot? 4x mag

Jar four - Wet spot 2? 4x mag

Jar five - 2x mag

Jar five - 4x mag

Please give me your opinion. Are these five jars doomed?
Edited by ashesofman (04/12/09 03:02 PM)
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MycoUnderground
Enthusiast



Registered: 10/23/08
Posts: 467
Loc: Suburban CO
Last seen: 12 years, 6 months
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Could be lipstick mold. You should look it up in the search and see if pictures of it match what you have. Sounds like you probably should toss it anyway. As far as I know metabolites are definately yellow. It could be making metabolites as a result of the red contam.
Edited by MycoUnderground (04/12/09 12:30 PM)
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ashesofman
Pulvis et umbra sumus



Registered: 03/18/09
Posts: 142
Loc: Norway
Last seen: 11 years, 20 days
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It doesn't look anything like lipstick mold. I'm really reluctant to get rid of jars that might still be okay.. If it was, could lipstick mold spores make it through the dry verm layer and micropore tape to infect other jars?
I really don't think it's lipstick mold though.
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MycoUnderground
Enthusiast



Registered: 10/23/08
Posts: 467
Loc: Suburban CO
Last seen: 12 years, 6 months
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It's unlikely that any contams made it through the tape and the verm. It is also unlikely that that jar would contaminte other jars, but if you feel uneasy about it just isolate it from the others. (Actually, I think that if you are using a dry verm layer you don't need the tape, but that is beside the point.)
If there were any contaminate spores it would be there from surviving PC'ing. Did you PC for 90 mintues?
You should look up "red spots in jar" or other related things in the search to see if you can find something similar to what you have.
Even if it is not lipstick mold, it sounds like contam.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Metabolites can and often are red, orange, brown or yellow. If it's liquid, it CAN'T be mold. See the picture below of metabolites on the top of a brf jar when the lid was removed. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Brennus
Student of Life



Registered: 05/31/08
Posts: 3,297
Loc: SE United States
Last seen: 5 years, 11 months
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Rust brown/yellow stains are metabolites. Blood red spots definitely sound like some sort of lipstick or other mold. I doubt anyone can tell you much without pictures.
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ashesofman
Pulvis et umbra sumus



Registered: 03/18/09
Posts: 142
Loc: Norway
Last seen: 11 years, 20 days
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I PCed for 60 minutes. If it is a contam, it's a wonder I haven't seen it until now. These jars are 19 days old and almost fully colonized. The weird thing is that the mycelium continues to grow in these jars. They haven't slowed to any noticible degree. Is it normal for the mycelium to ignore contams?
I took some pictures using a digital macro setting, but I had to take them at 2x, which made the spot seem huge. I looked at my jars again, and I'm noticing very, very small red spots on a few of them. They're maybe .1mm in diameter. They're barely visible to the naked eye.
I really hope this isn't a contam.
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ashesofman
Pulvis et umbra sumus



Registered: 03/18/09
Posts: 142
Loc: Norway
Last seen: 11 years, 20 days
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bump for edit
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scoops
Stranger


Registered: 03/10/09
Posts: 117
Last seen: 13 years, 2 months
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Quote:
RogerRabbit said: Metabolites can and often are red, orange, brown or yellow. If it's liquid, it CAN'T be mold. See the picture below of metabolites on the top of a brf jar when the lid was removed. RR

wow, VERY red metabolites! I think thats what's happening in your jars, ashesofman. I don't see how it could be a contaminant if it's only showing up where there is already strong mycelium. I've only had some very yellow metabolites show up in my jars so far.
I do believe that jar in RR's pic is an oyster cake. So says the link to the picture. But I'm sure what he said about the colors still applies to P. Cubensis and other species.
-------------------- "Ironic, that those who consider us mad are the ones who choose to live in the madness of the illusion"
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Psuper
Psilocybin

Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 5 months
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I know this thread is from a few months ago; just wanted to bring up red metabolites again....
I've only seen red metabolites once. It was on a single tray that was spawned with a high spawn ratio. Only once over the course of numerous projects through the past year.
Is it fairly uncommon? Do we have any idea what causes this? ~Pixie~
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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whimsy
Fairy



Registered: 06/09/09
Posts: 68
Loc: Tír na nÓg
Last seen: 9 years, 8 days
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Re: Red metabolites [Re: Psuper]
#10642251 - 07/08/09 02:44 PM (14 years, 7 months ago) |
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My jars are experiencing something that looks very similar, but in the contam forum I was told it was most likely rust flecks (this is my first grow and I drilled holes in my lids and didn't rinse the jars before I added my substrate). The rust, if that's indeed what it is, has not grown in my jars and it's been over a week, maybe 2 now, since they first showed up. Looks just like those pics above... so maybe it's just rust?
--------------------
 The woods are full of fairies! The trees are alive: The river overflows with them. See how they dip and dive! What funny little fellows! What dainty little dears! They dance and leap, and prance and peep, And utter fairy cheers!
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Psuper
Psilocybin

Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 5 months
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Re: Red metabolites [Re: whimsy]
#10642278 - 07/08/09 02:50 PM (14 years, 7 months ago) |
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No, I know that what I was looking at was red metabolites, like RR shows above in the pic. Iam not looking for a diagnosis.....
I am asking if we know a specific cause, and if it's fairly uncommon or not. ~Pixie~
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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Psuper
Psilocybin

Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 5 months
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Re: Red metabolites [Re: Psuper]
#10652385 - 07/10/09 11:20 AM (14 years, 7 months ago) |
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Quote:
Psuper said: I know this thread is from a few months ago; just wanted to bring up red metabolites again....
I've only seen red metabolites once. It was on a single tray that was spawned with a high spawn ratio. Only once over the course of numerous projects through the past year.
Is it fairly uncommon? Do we have any idea what causes this? ~Pixie~
friendly bump.
-------------------- Clinical Management of High Dose Psilocybin Sessions: https://www.youtube.com/watch?v=FbHOTIqjZLk
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smurpht
Stranger

Registered: 08/14/09
Posts: 107
Last seen: 12 years, 11 months
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Re: Red metabolites [Re: Psuper]
#10911342 - 08/22/09 11:34 AM (14 years, 6 months ago) |
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i am experiencing this problem right now, or is it not a problem? i'm confused.. could someone explain metabolites please. i read they are anti bodies, should i still dunk and roll?? they are ready to come out right now , the brf cakes.
it looks like the jar 4 and jar 5 pics he posted up there.
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smurpht
Stranger

Registered: 08/14/09
Posts: 107
Last seen: 12 years, 11 months
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Quote:
RogerRabbit said: Metabolites can and often are red, orange, brown or yellow. If it's liquid, it CAN'T be mold. See the picture below of metabolites on the top of a brf jar when the lid was removed. RR

what do metabolites do besides being an antibody, i do not understand this i'm a beginner. is it still safe to dunk and roll now, mine look like jars 4 and 5 in his pics and are still in the jars , i was scared to take them out. so i let it consolidate for a few days.
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Heisencube
Heisencube

Registered: 10/08/22
Posts: 11
Loc: London, UK
Last seen: 1 year, 2 months
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Hello Mr RR
I have a tray of bulk sub, cubes, that is developing a pink tint all over the place. There is no smell, and it mostly looks fine other than the pink haze. The only thing I have done differently with this tray is that I had two grain jars left over, a Hawaiin PES and a Mezatec, that I mixed together to create the tray.
But I always thought it was fine to mix these, since they are all cubes. I have done this many times before and have never had a problem. I cannot figure out how to post pictures, but I do have 4 good pictures. Any thoughts?
-------------------- Happy to be joining you folks from the UK
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DERRAYLD
Constructus


Registered: 05/13/02
Posts: 9,574
Loc: South Africa
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Pink is never good.
This thread is from 2009.
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Badvodoo
Stranger


Registered: 05/23/17
Posts: 22
Last seen: 9 months, 8 days
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Re: Red metabolites? [Re: DERRAYLD]
#28142008 - 01/15/23 08:39 PM (1 year, 1 month ago) |
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It was from 2009 but in my case it was still the most revelant answer to my questions even in 2023
I have some white oysters jars that had been laying around for a while and were way over colonized and i wanted to fruit them straight from the jar because i had not much interest in making them in bulk
(I made bags of wood fuel pellets for my mother as a christmas gift she wanted kings and regular oysters for a while ) but i was left with some unused jars since november
The kings started to pin in the jars and made no metabolites but the white oyster were way overcolonized and were choking themself by blocking the polyfill full of mycelium and created a closed cocoon that i ripped open by looking inside, it was full of metabolites and some was a very bright red ...it could be pigments from the rust of my lids mixing a bit in but i highly doubt it ...here is some pics
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