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OfflineLunarSpaceDream
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Registered: 04/04/09
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Last seen: 13 years, 4 months
sad sad sad sad....
    #10137400 - 04/09/09 04:09 PM (14 years, 10 months ago)

my first try ended up with contam.... im so very sad =(    i still have 3 jars that don't stink and i'm hoping they still have a shot but i highly doubt it. the reason i say that is because i had them all in a sterile container, and if 5 out of 8 smelled horrible, i don't think the other 3 made it. but i'm keeping them just in case. 

i followed the pf tek with the brf. but instead of pc, i just steam cooked them. the next time i do it, i'm going to put tin foil back over the top as soon as i'm done noc'ing them and hopefully that will reduce the chances of contamination.... but one thing i have to ask, do you guys think that putting them all in one container was my downfall, or was it just poor sterilization methods?    if so what do you recommend as far as keeping it all as sterile as possible


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Don't panic, we'll all be dead soon anyway. enjoy it while it lasts


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Offlinebchunter
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Re: sad sad sad sad.... [Re: LunarSpaceDream]
    #10137465 - 04/09/09 04:18 PM (14 years, 10 months ago)

did you use a glovebox?? yea the tinfoil should be off as little as possible to reduce the chance of contams floating in.
My first attempt i steamed and didnt use a glove box and all the jars got contams, next time i pc'd and used a glove box
and no contams. did you also flame the needle each time you injected the each jar?


Edited by bchunter (04/09/09 04:19 PM)


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OfflineLunarSpaceDream
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Re: sad sad sad sad.... [Re: bchunter]
    #10137490 - 04/09/09 04:23 PM (14 years, 10 months ago)

i did not, but i plan to next time for sure. and yea i did flame the needle before each jar. i just hope next time works better....


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Don't panic, we'll all be dead soon anyway. enjoy it while it lasts


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Offlinebchunter
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Re: sad sad sad sad.... [Re: LunarSpaceDream]
    #10137642 - 04/09/09 04:44 PM (14 years, 10 months ago)

i think the key is a good glovebox to prevent contamination, I was lazy the first time too but it's worth it to put in
that little extra effort to watch mushrooms grow instead of watching a failed attempt


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OfflineLunarSpaceDream
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Re: sad sad sad sad.... [Re: bchunter]
    #10137657 - 04/09/09 04:46 PM (14 years, 10 months ago)

haha yea...


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Don't panic, we'll all be dead soon anyway. enjoy it while it lasts


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OfflineHiddenIdentity
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Registered: 04/03/09
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Re: sad sad sad sad.... [Re: LunarSpaceDream]
    #10137795 - 04/09/09 05:09 PM (14 years, 10 months ago)

Quote:

yea the tinfoil should be off as little as possible to reduce the chance of contams floating in.




  Not true, the tinfoils purpose is to keep excess moisture from getting in.  You should use tyvec envelopes or coveralls to put over the top of the lid.

  Also I agree, the glovebox is essential.  Just take a big cardboard box with the top cut off.  Then place clear plastic over that, tape the back end down, and let it hang over the front.  Make 2 armholes just big enough for your arms.  Put everything you are going to be using to knocc up the jars in the box, then spray the hell out of it with lysol.  Wait 5-10 mins with the top of the box covered by the plastic, AND the armholes covered by the plastic as well.

  Wash your hands, and put on gloves.  Wipe the gloves down with alcohol.  Put your arms in the box, and let the plastic go all around your arms so there is less opening around your arms.  Then do everything as fast as possible, flame the needle between jars to prevent cross contamination, then seal the jars up as fast as possible.

  For the inoculation points, poke right through the tyvek, and replace the hold with a small sterile band-aid.


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Offlinerajajuju
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Registered: 12/30/08
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Re: sad sad sad sad.... [Re: HiddenIdentity]
    #10138008 - 04/09/09 05:36 PM (14 years, 10 months ago)

you know how to skip all that? 

grow bags

http://www.themadseason.com/

im about to harvest my second crop, this time i grew some PE - ate about 6gms dry the other day.. was sooooo happy with them.. it was like i lived 100 years in 2 hours, then phased out into the negative time stream and unraveled the fate of the universe
:psychsplit:

got like 10 more pins popping up in past 2 days

one thing i did differently is to wait until the bag was fully colonized with mycelium (solid white block), then i cut open the bag and put the colonized cake into an aquarium (highly recommended - gives the mushrooms plenty of room to grow, if you keep it in the bag it becomes a big mess of crap)


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Offlinec1dh3d
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Re: sad sad sad sad.... [Re: HiddenIdentity]
    #10138036 - 04/09/09 05:41 PM (14 years, 10 months ago)

The only thing I really use to keep things sterile is lysol, rubbing alcohol, and face masks - and it seems to have worked out so far. However, when I say I use lysol, I mean I really use lysol.. a lot. Haven't had a trich problem yet before my first couple of flushes, and I attribute the eventual trich setting in due to the substrate shrinking and allowing side pins to grow and eventually rot.


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InvisibleShroominit
Part Time Mycologist

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Re: sad sad sad sad.... [Re: c1dh3d]
    #10138086 - 04/09/09 05:49 PM (14 years, 10 months ago)

Lots of strange info in here, I'm sure it could work, but... I'm not sure why you'd do it that way.

Mix up your jars per the PF Tek recipe, add a 1/2" dry verm layer to the top, put the lids (with holes) on, place foil over the lids and sterilize.

Once sterilization is complete, remove foil.

Once cooled, inoculate (flame needle every time).

Then Enjoy.

If you can get a pressure cooker, use that instead of boiling. It is much better.


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Offlinem08b08
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Registered: 01/14/09
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Re: sad sad sad sad.... [Re: Shroominit]
    #10138328 - 04/09/09 06:34 PM (14 years, 10 months ago)

you should really invest in a pressure cooker ! you cannot go wrong :thumbup:


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Offlinegrindshits
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Registered: 04/09/09
Posts: 1
Last seen: 14 years, 10 months
Re: sad sad sad sad.... [Re: m08b08]
    #10138337 - 04/09/09 06:36 PM (14 years, 10 months ago)

how terrible. it may be because you left them with the foil off on the closet in the open air.


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InvisibleShroominit
Part Time Mycologist

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Posts: 4,662
Re: sad sad sad sad.... [Re: grindshits]
    #10138342 - 04/09/09 06:37 PM (14 years, 10 months ago)

Quote:

grindshits said:
how terrible. it may be because you left them with the foil off on the closet in the open air.





Unlikely. I take the foil off at the end of sterilization and leave it off. Not a single PF jar to date has been contaminated.


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