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Amazon Shop: ½ Pint Jars, Pressure Cooker

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Offlinepimster987
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Registered: 10/02/02
Posts: 73
Last seen: 11 years, 7 months
how the hel
    #1013789 - 11/01/02 06:00 PM (14 years, 9 months ago)

this ismy second time inculating and both times i gotcontams what am i doin wrong. i put them in a cabinet with a heating pad on top seperated with a twoe in the middle so they dont touch jars.im sure was pretty sterile. i sterilize jars for 1 hour then inculated and never took lids off.


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OfflineTheHobbit
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Registered: 09/04/02
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Re: how the hel [Re: pimster987]
    #1017913 - 11/03/02 10:20 AM (14 years, 9 months ago)

Was it disinfected pretty well where you store them to incubate? Is the heating pad necessary, or could the room just be kept warm? Did you use a pressure cooker, or just use a steamer (as i do)? I'm a newbie too, and things look pretty good ingeneral, although a couple have a few small black spots (which i posted about as well).


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OfflineMAIA
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Re: how the hel [Re: pimster987]
    #1017993 - 11/03/02 11:04 AM (14 years, 9 months ago)

Do you flame & clean the syringe between innoclutions ?

MAIA


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Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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OfflineTheHobbit
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Re: how the hel [Re: MAIA]
    #1018783 - 11/03/02 04:49 PM (14 years, 9 months ago)

MAIA, should you sterlize the needle in between each innoculation for every hole in the lid? I do, but was wondering if it was necessary.


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Offlinepixelscope
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Registered: 10/29/02
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Re: how the hel [Re: TheHobbit]
    #1019164 - 11/03/02 09:17 PM (14 years, 9 months ago)

flame sterlize between each jar,,,each hole would for one be very time consuming and not nessesary,,, if one injection hole contams from the needle, cleaning it after that wont help that jar,,but if you sterlize between jars you can stop the spread.


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OfflineMAIA
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Re: how the hel [Re: TheHobbit]
    #1023374 - 11/05/02 07:06 AM (14 years, 9 months ago)

pixelscope explanation sums it all.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
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Offlinepimster987
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Registered: 10/02/02
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Re: how the hel [Re: MAIA]
    #1025646 - 11/05/02 10:06 PM (14 years, 9 months ago)

maybe i didnt sterilize enough.


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Offlinerhizo
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Re: how the hel [Re: pimster987]
    #1027459 - 11/06/02 11:04 AM (14 years, 9 months ago)

When you say you sterilized the jars for one hour, did you use a pressure cooker?


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InvisibleLordSenate
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Re: how the hel [Re: rhizo]
    #1027462 - 11/06/02 11:06 AM (14 years, 9 months ago)

i never used a pressure cooker, and i hardly ever got contams maybe 3 jars out of 30


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Amazon Shop: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Contamination Forum

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