Home | Community | Message Board


Out-Grow.com - Mushroom Growing Kits & Supplies
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Amazon Shop for: ½ Pint Jars, Pressure Cooker

Jump to first unread post. Pages: 1
Offlinepimster987
journeyman
Registered: 10/02/02
Posts: 73
Last seen: 10 years, 11 months
how the hel
    #1013789 - 11/01/02 06:00 PM (14 years, 1 month ago)

this ismy second time inculating and both times i gotcontams what am i doin wrong. i put them in a cabinet with a heating pad on top seperated with a twoe in the middle so they dont touch jars.im sure was pretty sterile. i sterilize jars for 1 hour then inculated and never took lids off.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
Loc: the Oily Way...
Last seen: 13 years, 2 months
Re: how the hel [Re: pimster987]
    #1017913 - 11/03/02 10:20 AM (14 years, 1 month ago)

Was it disinfected pretty well where you store them to incubate? Is the heating pad necessary, or could the room just be kept warm? Did you use a pressure cooker, or just use a steamer (as i do)? I'm a newbie too, and things look pretty good ingeneral, although a couple have a few small black spots (which i posted about as well).


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,275
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 6 months, 9 days
Re: how the hel [Re: pimster987]
    #1017993 - 11/03/02 11:04 AM (14 years, 1 month ago)

Do you flame & clean the syringe between innoclutions ?

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTheHobbit
Pot Head Pixie

Registered: 09/04/02
Posts: 863
Loc: the Oily Way...
Last seen: 13 years, 2 months
Re: how the hel [Re: MAIA]
    #1018783 - 11/03/02 04:49 PM (14 years, 1 month ago)

MAIA, should you sterlize the needle in between each innoculation for every hole in the lid? I do, but was wondering if it was necessary.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepixelscope
Merry Pranksta
Registered: 10/29/02
Posts: 59
Loc: your moms house
Last seen: 12 years, 6 months
Re: how the hel [Re: TheHobbit]
    #1019164 - 11/03/02 09:17 PM (14 years, 1 month ago)

flame sterlize between each jar,,,each hole would for one be very time consuming and not nessesary,,, if one injection hole contams from the needle, cleaning it after that wont help that jar,,but if you sterlize between jars you can stop the spread.


--------------------
To infinity and beyond


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMAIA
World-BridgerKartikeya (DftS)
Male User Gallery

Registered: 04/27/01
Posts: 7,275
Loc: Erra - 20 Tauri - M45 Sta...
Last seen: 6 months, 9 days
Re: how the hel [Re: TheHobbit]
    #1023374 - 11/05/02 07:06 AM (14 years, 1 month ago)

pixelscope explanation sums it all.

MAIA


--------------------
Spiritual being, living a human experience ... The Shroomery Mandala



Use, do not abuse; neither abstinence nor excess ever renders man happy.
Voltaire


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinepimster987
journeyman
Registered: 10/02/02
Posts: 73
Last seen: 10 years, 11 months
Re: how the hel [Re: MAIA]
    #1025646 - 11/05/02 10:06 PM (14 years, 1 month ago)

maybe i didnt sterilize enough.


Post Extras: Print Post  Remind Me! Notify Moderator
Offlinerhizo
herb eater

Registered: 10/31/02
Posts: 598
Loc: Asia
Last seen: 9 years, 8 months
Re: how the hel [Re: pimster987]
    #1027459 - 11/06/02 11:04 AM (14 years, 1 month ago)

When you say you sterilized the jars for one hour, did you use a pressure cooker?


--------------------
An optimist is never pleasantly surprised.


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleLordSenate
One of the Lost
Male User Gallery

Registered: 09/15/02
Posts: 37,093
Loc: First Circle of Hell
Re: how the hel [Re: rhizo]
    #1027462 - 11/06/02 11:06 AM (14 years, 1 month ago)

i never used a pressure cooker, and i hardly ever got contams maybe 3 jars out of 30


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: 1

Amazon Shop for: ½ Pint Jars, Pressure Cooker

Mushrooms, Mycology and Psychedelics >> Contamination Forum

Similar ThreadsPosterViewsRepliesLast post
* pressure cooker hugo 1,585 16 06/29/04 06:42 AM
by MAIA
* Presure cooker PSI gauge ArmedLiberals 761 3 09/24/01 03:34 PM
by shellacct
* pressure cooker gets funky starptv23 907 8 03/11/05 11:12 PM
by starptv23
* Other than a Pressure cooker... ihy2 318 0 12/27/07 05:18 PM
by ihy2
* Jar Contam and Pressure cooker Dilauded 956 4 12/28/02 03:09 PM
by Suntzu
* besids a pressure cooker, how can i fight contams? newcultivator 598 1 08/26/03 06:49 PM
by Paid
* Myc. not fully colonizing in rye-grain jars after being PC'd rickaston 8,363 14 05/26/09 09:13 PM
by rickaston
* Everything contaminated?? SampleInAJar 1,012 7 11/11/02 04:22 PM
by New Name

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: RogerRabbit, cronicr, Pastywhyte
739 topic views. 1 members, 4 guests and 2 web crawlers are browsing this forum.
[ Toggle Favorite | Print Topic | Stats ]
Search this thread:
Edabea
Please support our sponsors.

Copyright 1997-2016 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.005 seconds on 14 queries.