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Mykologist
Often too lazy to post...



Registered: 10/06/08
Posts: 1,586
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Agar is not level, problem?
#10118866 - 04/06/09 07:23 PM (15 years, 9 months ago) |
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maybe this belongs in a different thread, like Advanced Myc...but i'm not entirely sure. Some dude I know has prepared some potato dextrose agar. he mixed it in water, boiled it for one minute, then pc'd the agar in a flask for 15 minutes. He poured four petri dishes, 60 mm diameter, 15 mm tall. He left, came back thirty minutes later and started inverting the dishes when he noticed the agar set crooked. How crooked? pretty damn crooked. One end is about 1mm thick, the other, 7mm. Agar melts at 50celsius (122 Fahrenheit). First of all, is it a problem? Second, what method do you guys recommend he re-heat up the agar to level it out? Third, the plates are sealed with mocopore tape. Is this suitable? I've read somewhere that's what one should use. If he microwaved the plates for a few seconds, would the tape get messed up? Suck in contaminants? (Agar is peroxilated) thanx for any advice Myk
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Groomies
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Registered: 08/16/07
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Re: Agar is not level, problem? [Re: Mykologist]
#10118930 - 04/06/09 07:31 PM (15 years, 9 months ago) |
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IMO: shouldn't be a prob, it doesn't have to be level.
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ScavengerType


Registered: 01/24/08
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Re: Agar is not level, problem? [Re: Groomies]
#10119003 - 04/06/09 07:41 PM (15 years, 9 months ago) |
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The first dishes I did weren't level it was no problem. Hell the agar was already semi-gelatinous when I went to poor it and it didn't even extend to the end of the dish. Haha, it still worked though. Now I wait the right amount of time to pour and I put my agar on a flat surface to harden.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
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Mykologist
Often too lazy to post...



Registered: 10/06/08
Posts: 1,586
Loc: I'm still here...
Last seen: 9 months, 18 days
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Thanx for your guys' responses. My man said fuck it! He forgot to peroxilate the agar BEFORE it coagulated, so it had fluid on top of it; it wasn't level, he ended up starting over. It was what, 1.8 grams agar?!?! If there is one thing growing mushrooms has taught him, it's that there really is no short-cut. it's best to do things right the first time through. Trying to correct stuff will fuck one over in the future, after much more effort and sweat has been spent. he'll try againg tomoro or the day after, he's pretty busy and all:) Thanx for your time though guys! Myk
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