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ChrisWho
Psilosopher


Registered: 03/06/08
Posts: 426
Last seen: 12 years, 7 months
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Never Been so Disappointed!
#10114464 - 04/06/09 02:06 AM (15 years, 9 months ago) |
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Last night I was about to prepare my casing mix for 2 double tubs that I'0ve been working with... Come to find out trich got both of them. I've never been so disappointed in my life! It was around the sides, and I couldn't see it, because they were stuffed in a closet, and I was only using a small flash light to check them... The problem is I don't know where I went wrong. I'll put out a few of my ideas and you guys can tell me what you think.
1.) I pasteurized my sub in a pillowcase, and left it to drain in the sink while I was at work....
2.) After spawning I failed to cut off the bag that stuck up over the sub. The bag ended up laying down on the sub. The reason I think this may be a problem is because the places where the bag was hanging over were affected the most.
3.) The coffee grounds... When adding coffee grounds to my sub I chose to just boil it in a pan of water a couple times instead of making a bunch of pots of coffee. I guess this could have been the source of the problem...
What do you guys think? I buried my sub outside, but I don't think it stands much of a chance as cold as it is out there. I went ahead and threw the tubs away rather than bothering with them... I also cleaned the whole house and Ousted the shit out of everything, and am now soaking some new WBS. I made an lc with the spores I used for the last attempt, so at least it wasn't a complete loss. I think this go around I may make a grow log... We'll see
-------------------- Drugs don't have spiritual potential, human beings have spiritual potential. And it may be that we need techniques to move us in that direction, and the use of psychoactive drugs clearly is one path that has helped many people.
-Andrew Weil
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demonicaeroponic
Ghost


Registered: 07/20/08
Posts: 276
Last seen: 13 years, 11 months
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Re: Never Been so Disappointed! [Re: ChrisWho]
#10114540 - 04/06/09 02:41 AM (15 years, 9 months ago) |
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boo, no pin porn.
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ChrisWho
Psilosopher


Registered: 03/06/08
Posts: 426
Last seen: 12 years, 7 months
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Believe me, I'm more upset than you are.
-------------------- Drugs don't have spiritual potential, human beings have spiritual potential. And it may be that we need techniques to move us in that direction, and the use of psychoactive drugs clearly is one path that has helped many people.
-Andrew Weil
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BaKeRx561x420
Pound 'em till you piss



Registered: 09/28/07
Posts: 368
Loc: Nowhere, Kansas
Last seen: 12 years, 9 months
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Re: Never Been so Disappointed! [Re: ChrisWho]
#10114573 - 04/06/09 03:09 AM (15 years, 9 months ago) |
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Quote:
ChrisWho said: I don't know where I went wrong.
Quote:
I made an lc with the spores
Bingo. Your spawn was contam'd from the start, and you didn't find out till way down the road. Stick with MS or learn some basic agar skills.
-------------------- Get some experience under your belt or shut the fuck up.
RR
FUCK YOU TRICHODERMA
 
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ChrisWho
Psilosopher


Registered: 03/06/08
Posts: 426
Last seen: 12 years, 7 months
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No, no... I made my jars, and then I made an LC with the spore solution I had left, which I'll be using for my next jars. Sorry for the confusion.
-------------------- Drugs don't have spiritual potential, human beings have spiritual potential. And it may be that we need techniques to move us in that direction, and the use of psychoactive drugs clearly is one path that has helped many people.
-Andrew Weil
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Ganja420Boy
Heroic Doser



Registered: 11/15/06
Posts: 1,207
Loc: CoSmoS
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Re: Never Been so Disappointed! [Re: ChrisWho]
#10114586 - 04/06/09 03:20 AM (15 years, 9 months ago) |
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Did you open the tub up any while it was still colonizing ?
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ChrisWho
Psilosopher


Registered: 03/06/08
Posts: 426
Last seen: 12 years, 7 months
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Re: Never Been so Disappointed! [Re: Ganja420Boy]
#10114595 - 04/06/09 03:25 AM (15 years, 9 months ago) |
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Negative... I had them gorilla taped together
-------------------- Drugs don't have spiritual potential, human beings have spiritual potential. And it may be that we need techniques to move us in that direction, and the use of psychoactive drugs clearly is one path that has helped many people.
-Andrew Weil
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c1dh3d
The elephant is BACK




Registered: 07/15/08
Posts: 5,229
Loc:
Last seen: 1 year, 7 months
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Re: Never Been so Disappointed! [Re: ChrisWho]
#10114633 - 04/06/09 03:45 AM (15 years, 9 months ago) |
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If the most disappointed you have ever been in your entire life, is with this encounter with Trich, consider yourself lucky
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rugergirl79
lεṡвïαṉ εχτɾøḋïṉ^



Registered: 03/27/08
Posts: 2,113
Loc: cardboard box
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Re: Never Been so Disappointed! [Re: ChrisWho]
#10114651 - 04/06/09 03:53 AM (15 years, 9 months ago) |
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i have read that if you get contaims before your first flush, your inoculation, or spore syringe technique needs working on. It could have possibly been the print or syringe that you were working with, but if i had to put money on it, inoculation is where it got you.
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ChrisWho
Psilosopher


Registered: 03/06/08
Posts: 426
Last seen: 12 years, 7 months
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Re: Never Been so Disappointed! [Re: c1dh3d]
#10114655 - 04/06/09 03:54 AM (15 years, 9 months ago) |
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It really wouldn't have upset me so much, but it took my jars over a month to fully colonize, and then the spawn run took over two weeks, and I was all hot in the pants about casing, only to find out I had trich... Who knows how long it was on there
-------------------- Drugs don't have spiritual potential, human beings have spiritual potential. And it may be that we need techniques to move us in that direction, and the use of psychoactive drugs clearly is one path that has helped many people.
-Andrew Weil
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shroomzey
Humble Student




Registered: 05/17/08
Posts: 904
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Re: Never Been so Disappointed! [Re: ChrisWho]
#10114786 - 04/06/09 04:43 AM (15 years, 9 months ago) |
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Describe your pasteurization method?
--------------------
200 years from now, we will look back and laugh at ourselves for how stupid we were.
My Glovebox
Find a respected member of the community and study them. I give thanks to: RogerRabbit, agar, hyphae, Nibin, fahtster, The shroomy 1, monstermitch, FooMan, HippieChick, Blue Helix, eatyualive, mycofile, and many, many more.
Never accept mediocrity, always return the love that is given to you.
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ChrisWho
Psilosopher


Registered: 03/06/08
Posts: 426
Last seen: 12 years, 7 months
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Re: Never Been so Disappointed! [Re: shroomzey]
#10114837 - 04/06/09 05:19 AM (15 years, 9 months ago) |
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Well, I had alot of sub to pasteurize, so I had to split it up into two parts, since I didn't have a pot big enough.
I loaded the sub up into a pillowcase, and put into a pot of water. I then brought the pot up to around 165 and turned the heat to it's lowest setting. I left it there for an hour, checking occasionally to make sure the temp stayed up. After pasteurizing the first bag I twisted the bag as tightly as I could over the bath tub, and then put it into a strainer over the sink. The process was the same with the second bag, except maybe it wasn't squeezed as well, and it sat in the strainer for the time I was at work... Then I spawned each tub with 6 qts. of colonized WBS... I'm starting to think the coffee may have been the biggest, if not the only problem. Should I have dried it out before adding it to my sub?
-------------------- Drugs don't have spiritual potential, human beings have spiritual potential. And it may be that we need techniques to move us in that direction, and the use of psychoactive drugs clearly is one path that has helped many people.
-Andrew Weil
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Starrider
Padawan


Registered: 11/09/08
Posts: 499
Loc: Between here and there
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Re: Never Been so Disappointed! [Re: ChrisWho]
#10115949 - 04/06/09 10:52 AM (15 years, 9 months ago) |
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You really should use agar to start the inoculation process. If the plate is clean then you can proceed. If the plate has trich, you can cut the good mycelium out and continue by separating the good from the bad. That is, continue growing on plates until you have a clean plate. No worries mate.
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"If the doors of perception were cleansed every thing would appear to man at it is, infinite."
William Blake
Anything that I write is for fun only. I am creating fiction for the pleasure of my readers.
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5HTSynaptrip
Dopamine Enthusiast




Registered: 09/14/08
Posts: 4,360
Loc: USA
Last seen: 6 years, 8 months
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Re: Never Been so Disappointed! [Re: Starrider]
#10116014 - 04/06/09 11:02 AM (15 years, 9 months ago) |
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I'd have to say insufficient amount of spawn used? How much substrate vs spawn?
The only time I've contamed was using a poorly pasteurized coir casing, I used it too soon and below I didn't use enough spawn.
Since then I've been using 2 quart jars per tub and haven't had any problems. I don't case either though anymore.
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Science is a way of thinking much more than it is a body of knowledge. - My hero, who will be forever remembered, Carl Sagan.
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hemppy
Stranger
Registered: 02/25/09
Posts: 100
Last seen: 6 years, 7 months
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Re: Never Been so Disappointed! [Re: 5HTSynaptrip]
#10116169 - 04/06/09 11:23 AM (15 years, 9 months ago) |
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It could be a number of things. You said you only left your sub in the 165 degree water for 1 hour? Well as RR says you need to make sure the middle of the sub bag is at the correct temp for 1 hour. I doubt your sub even got close to 160 in the middle and certainly not for an hour. I have been using oven bags for my sub in 170 water and it has taken at least 3 hours for my sub to hit 160 in the middle. Without a lot more info I would say that is where the contam came from.
You said your jars were not contamed so it had to come from improper pasteurizing or the tubs/bags you lined the tubs with were already contamed.
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Captain Caveman
Uh-oh!



Registered: 01/12/09
Posts: 2,253
Last seen: 1 year, 24 days
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Re: Never Been so Disappointed! [Re: hemppy]
#10116296 - 04/06/09 11:42 AM (15 years, 9 months ago) |
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OR it was just the simple act of mixing in the spawn. OR, his sub wasnt fully pasteurized. OR it was them being "stuffed in a closet" that may be full of contams, that somehow made it past his filter for GE.
It could be many things guys. And OP, dont let this bring you down. It happens. Hell, I have an unknown contam that I have been battling from this years start. But, I am learning alot, and I will admit. Its kind of cool when nobody knows what the hell it is All Im saying is, shit happens. Just clean everything, and try and try again.
-------------------- "Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"
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shroomzey
Humble Student




Registered: 05/17/08
Posts: 904
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I'm leaning towards the bulk being under pasteurized... I'll pasteurize 4 gallon loads of bulk sub at a time, and I used a 5 gallon paint strainer to do this. I suggest you pick one from Home Depot or Lowes or the such, they're very handy. They make handling with grain easy as well.
Anywho, you really need a temperature gauge that can pierce into the core of the substrate. When I pasteurize on the stove you need to agitate the bag while it is in the pot, get that heated water to penetrate the sub. The temperature of the water surrounding the bag will be at 165F, but the core will be 125, 130. Get some rubber gloves that can handle the heat, and make sure you agitate the bag until your probe really shows you that the core is within range.
--------------------
200 years from now, we will look back and laugh at ourselves for how stupid we were.
My Glovebox
Find a respected member of the community and study them. I give thanks to: RogerRabbit, agar, hyphae, Nibin, fahtster, The shroomy 1, monstermitch, FooMan, HippieChick, Blue Helix, eatyualive, mycofile, and many, many more.
Never accept mediocrity, always return the love that is given to you.
Edited by shroomzey (04/06/09 02:14 PM)
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FryinHard
Hamburgerler


Registered: 02/16/04
Posts: 361
Loc: McDonalds
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Re: Never Been so Disappointed! [Re: shroomzey]
#10117122 - 04/06/09 02:16 PM (15 years, 9 months ago) |
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Did you use any sort of heating device to incubate the sub?
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Captain Caveman
Uh-oh!



Registered: 01/12/09
Posts: 2,253
Last seen: 1 year, 24 days
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Re: Never Been so Disappointed! [Re: shroomzey]
#10120087 - 04/06/09 10:19 PM (15 years, 9 months ago) |
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I have simplified my pasteurization over the years, to ditching thermometers and just mixing my dry materials with boiling water in a cooler and letting cool/cook overnight. Last time I checked with a thermometer it had reached 200F, which contrary to popular belief is NOT gonna cause harm.
-------------------- "Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"
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ChrisWho
Psilosopher


Registered: 03/06/08
Posts: 426
Last seen: 12 years, 7 months
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Thanks for all the replies guys. I've been thinking alot about it, and it could also be due to my sub being to wet when I spawned maybe... I could have pasteurized longer, but I am sure the middle of the sub reached 150, because I have a digital thermometer with a probe on it... I looked at wal-mart for a paint strainer, but they looked at me like I was retarded when I asked for help. I guess there may be a couple other places I could check, but I don't have a lowe's of Home Depot near me.... Thanks again guys... I'll be knocking up some new jars tomorro. Wish me luck.
-------------------- Drugs don't have spiritual potential, human beings have spiritual potential. And it may be that we need techniques to move us in that direction, and the use of psychoactive drugs clearly is one path that has helped many people.
-Andrew Weil
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