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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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broken syringe
#10114425 - 04/06/09 01:47 AM (15 years, 9 months ago) |
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HI all, just received a syringe in the post from a trusted vendor.
Unfortunately when I opened this the needle was snapped off the body of the syringe. It.'s in what looks like a cleanish zip lock bag,so whats my best plan to rescue this. Luckily, nearly all the liquid is still in the syringe.
Heres amy plan, Im thinking new empty syringe and shot glass wrapped in foil and PC's for 20 minutes, and basically knock up a new syringe out of the broken one.(in stelile glove box of course.)
YOu think this will work?
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Edited by FractalXplora (04/06/09 02:02 AM)
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Groomies
Ghost



Registered: 08/16/07
Posts: 1,119
Last seen: 8 years, 1 month
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yes that should work
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: broken syringe [Re: Groomies]
#10114457 - 04/06/09 02:04 AM (15 years, 9 months ago) |
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phew, I'll give it a go. Im sure fucking customs snapped this, the envelope was marked "mushroom research" seriously, thats fucked up!
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pftek
1337


Registered: 08/18/08
Posts: 832
Last seen: 14 years, 3 months
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Dude. Same thing happened to me but I was trying to sterilize the whole needle and the plastic caught on fire.
Take one of those half pint jars. PC water in it. Add some a tablespoon of Karo. Inject it with that. Now you got some Liquid Culture. 10x more potent than that multispore.
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ozzysmygod
Late Night PC'ing Enthusiast


Registered: 11/26/08
Posts: 835
Last seen: 10 years, 2 months
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Re: broken syringe [Re: pftek]
#10115015 - 04/06/09 07:09 AM (15 years, 9 months ago) |
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Quote:
pftek said: 10x more potent than that multispore.
To clarify, using an LC will NOT make your potency rise, It will just speed up colonization time.
secondly, the LC will still be multispore of sorts, as no sectoring or any other agar work will of been carried out, therefore there will be several substrains in one jar of LC
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German Kahuna
Facepalmer of Stoopid


Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Did you snap the needle off or did it arrive like that? If it arrived in that state, snap a pic of it and send it to the vendor by PM or email politely asking for a replacement.
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Yeah Kahuna, it was http://www.magicmushrooms.org/ I emailed them this morning got a response in about 30 minutes, and they will replace it no problems. Great service all credit to them. The broken one has been re-homed to, hopefully will be sterile, time will tell.
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Bugzy
Magician



Registered: 03/18/09
Posts: 436
Last seen: 11 years, 4 months
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Tell them to send it back in a unmarked envelope. I wouldnt want anything being shipped w/ a "mushroom research" tramp stamp.
-------------------- American by Nature.... unamerican by Law
"Out of all the things I've lost, I miss my mind the most."
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dead
grateful


Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 10 years, 2 months
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Re: broken syringe [Re: pftek]
#10115401 - 04/06/09 09:08 AM (15 years, 9 months ago) |
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Quote:
pftek said: Dude. Same thing happened to me but I was trying to sterilize the whole needle and the plastic caught on fire.
Take one of those half pint jars. PC water in it. Add some a tablespoon of Karo. Inject it with that. Now you got some Liquid Culture. 10x more potent than that multispore.
That's one lousy bit of advice.
For one thing, you should add the honey/karo/whatever-source-of-sugar BEFORE pressure cooking, otherwise your LC will NOT be sterile.
For another thing, you should NOT inject an entire syringe of spore solution into an LC. You only need a tiny drop of spore solution to inoculate an LC, and too many spores is bad.
And another, LC:s are tricky. There's no way to tell if the mycelium in an LC is mold or mushroom mycelium, so you will have to try it on 1 jar first.
-------------------- "The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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Krazy L
Resident Whack Job


Registered: 01/02/09
Posts: 85
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Re: broken syringe [Re: dead]
#10115698 - 04/06/09 10:03 AM (15 years, 9 months ago) |
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How is an lc "more potent" than multispore?
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T-Rex




Registered: 04/05/08
Posts: 4,185
Loc: NY
Last seen: 11 months, 21 days
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Re: broken syringe [Re: Krazy L]
#10116097 - 04/06/09 11:11 AM (15 years, 9 months ago) |
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Its not more potent just overall colonization speed is faster due to the mycellium already being present after inoculation
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