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Offlinecaseyroy
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Registered: 03/02/09
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Should i just grow in jars
    #10112207 - 04/05/09 06:38 PM (15 years, 9 months ago)

I keep getting trichoderma so should i just have the shrooms grow inside the jars rather than puting them in a FC?

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InvisibleDemonic_Chronic
The Plague Doctress
Trans-female


Registered: 08/10/08
Posts: 4,199
Loc: PNW Flag
Re: Should i just grow in jars [Re: caseyroy]
    #10112252 - 04/05/09 06:46 PM (15 years, 9 months ago)

you should move your grow room.

DC


--------------------
The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.

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OfflineScavengerType
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Registered: 01/24/08
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Re: Should i just grow in jars [Re: Demonic_Chronic]
    #10112290 - 04/05/09 06:55 PM (15 years, 9 months ago)

most contaminations are the results of inoculation problems. You should tell us more about how you are growing and what you are inoculating from.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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Offlinecaseyroy
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Re: Should i just grow in jars [Re: ScavengerType]
    #10112543 - 04/05/09 07:39 PM (15 years, 9 months ago)

Im doing the brf tek with a hawaiian strain from ralphsters. I been very in tune with my sterility precautions during inoculation with a glovebox. My first 6 jars fully colonized no contam. Gave em an extra week after full colonization, birth, dunk rolled, popped them in the fc with perlite 99% humidity 72 degrees, fanned about 6 to 8 times a day with misting, then within 5 days tricho sets in. Its killed 6 jars already. Now i just birthed some jars tonight and now theyre dunking in a pot of tap water. And when i roll should i sterilize verm maybe to help? And yeah im putting the fc in a different room this time. But im pretty doubtful im going to have any success cause of this tricho shit. so i really havent have a clue to what to do anymore

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Offlinepotatonet
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Registered: 11/13/08
Posts: 1,385
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Re: Should i just grow in jars [Re: caseyroy]
    #10112567 - 04/05/09 07:43 PM (15 years, 9 months ago)

go get some oust and a HEPA unit for your room.

the spores are in the air and the house. dont leave it in a room with a window that gets opened


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In the Quechua language of Peru there are over 1000 words for "potato"

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OfflineMykologist
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Re: Should i just grow in jars [Re: caseyroy]
    #10112572 - 04/05/09 07:43 PM (15 years, 9 months ago)

Move you FC into a new room, then BLEACH THE FUCK out of your shotgun.
  If you have a good vacuum, may not hurt to vacuum your new room while you're dunking your cakes.
Hope you avoid the trich!
myk
oh, and perhaps add some H2O2 to your misting water. Should keep trich spores at bay.


--------------------


Where would you rather be?

Edited by Mykologist (04/05/09 07:44 PM)

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Offlinepotatonet
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Re: Should i just grow in jars [Re: Mykologist]
    #10112620 - 04/05/09 07:54 PM (15 years, 9 months ago)

indeed with the h2o2


--------------------
In the Quechua language of Peru there are over 1000 words for "potato"

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OfflineScavengerType
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Registered: 01/24/08
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Re: Should i just grow in jars [Re: Mykologist]
    #10112723 - 04/05/09 08:10 PM (15 years, 9 months ago)

I have a hepa and run this in the room I use as my lab before doing anything in it. I do petri dishes in my GB in this room. I shut off the HEPA before I get to work so as to prevent airflow. I have done over 30 dishes under these conditions and despite having the dishes open in some times as long as 7 to 15 seconds I have had only 4 contaminants so far. I think HEPAs cleaning the air before you work help though I can't say I have proof other than my anecdotal experience.

I may ask, are you flame sterilizing your needles? Also I may suggest if you are worried about contaminants that you use micropore tape over the inoculation holes instead of or with a dry verm layer.

Usually for Tric to get a hold it needs to colonize the substrate (your cake) before the mushroom mycelium does. Now trich is only green when it spores so as it develops it looks white and you may not be able to recognize it until it is reached it's sporulating stage. You should IMO clean any area of your house where this was growing with bleach and/or vinegar as well as running a HEPA filter to get the spores out of the air.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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InvisibleDemonic_Chronic
The Plague Doctress
Trans-female


Registered: 08/10/08
Posts: 4,199
Loc: PNW Flag
Re: Should i just grow in jars [Re: ScavengerType]
    #10112787 - 04/05/09 08:22 PM (15 years, 9 months ago)

H202 does nothing for trich, H202 is for cobweb mold. at least im pree sure it doesnt do anything for trich.

DC


--------------------
The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.

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OfflineScavengerType
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Registered: 01/24/08
Posts: 5,784
Loc: The North
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Re: Should i just grow in jars [Re: Demonic_Chronic]
    #10112813 - 04/05/09 08:28 PM (15 years, 9 months ago)

it does not. I've even read on here that trich spores can't be killed by bleach or something similar. This is why I suggested vinegar.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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Offlinebbf
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Re: Should i just grow in jars [Re: caseyroy]
    #10112818 - 04/05/09 08:29 PM (15 years, 9 months ago)

I doubt this will solve your problem, but you might want to consider dunking each cake in a separate container (i.e. ziploc bags) as an extra precaution.

I hope you beat this problem, I feel for you. :frown:

Down with the mean green!


--------------------
Correlation does not imply causation.

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OfflineScavengerType
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Re: Should i just grow in jars [Re: bbf]
    #10112874 - 04/05/09 08:38 PM (15 years, 9 months ago)

If he's letting the cake full colonize it is unlikely that he is getting tric from the dunk. Particularly given the time line. Trich could set in on a bare uncolonized cake in 6 days but vs myc no fucking way. That trich had to be there from inoculation.


--------------------
"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

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Offlinetripz
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Registered: 03/27/05
Posts: 154
Last seen: 7 years, 3 months
Re: Should i just grow in jars [Re: bbf]
    #10112889 - 04/05/09 08:40 PM (15 years, 9 months ago)

You should spend some time to try to isolate and stop the root cause of the problem is stead of trying to find a way to fix the problem after it happens.

A) Change your AC filter regularly (I do every 30 days). If possible, invest in a UV lamp for your ductwork.
B) Search your home for wet spots. Check behind dish washer, sinks, washing machines, boiler, tub, under refrigerator, etc..
C) Invest in a small ozone generator and nuke your room every once in a while. Follow directions! O3 = nasty.
D) Stop all airflow when you're working in the open. Cut AC and fans.
E) Bleach/Lysol down your counter tops, kitchen sink, stove top, cabinets, etc.. a half an hour before you use your kitchen to prep/inoculate.


--------------------
irc.danknug.com
#shroomery / #cultivation

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