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pftek
1337


Registered: 08/18/08
Posts: 832
Last seen: 14 years, 3 months
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Nothing growing on WBS jars - Resterilize or Just reinject?
#10111518 - 04/05/09 04:53 PM (15 years, 9 months ago) |
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I dont see any mold or bacteria growing. I think the solution was just bad as all the other jars are doing okay just the ones using a different syringe.
Should I resterilize in a PC or just reinject? I was using multispore now I'd be injecting with LC.
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T-Rex




Registered: 04/05/08
Posts: 4,185
Loc: NY
Last seen: 11 months, 21 days
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: pftek]
#10111647 - 04/05/09 05:14 PM (15 years, 9 months ago) |
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I would start with new substrate. I've always ended up witha contam by resterilizing a jar.
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NineInchNails
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Registered: 03/01/09
Posts: 1,190
Last seen: 2 years, 12 days
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: T-Rex]
#10111662 - 04/05/09 05:17 PM (15 years, 9 months ago) |
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How long ago did you inoculate?
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pftek
1337


Registered: 08/18/08
Posts: 832
Last seen: 14 years, 3 months
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: NineInchNails]
#10111723 - 04/05/09 05:26 PM (15 years, 9 months ago) |
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like 2 weeks ago. it still may show something as it has been taking that long for almost all of my jars. But just in case - plus my lc is almost ready.
Edited by pftek (04/05/09 05:27 PM)
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T-Rex




Registered: 04/05/08
Posts: 4,185
Loc: NY
Last seen: 11 months, 21 days
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: pftek]
#10111763 - 04/05/09 05:31 PM (15 years, 9 months ago) |
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I'd give it another week. 3 weeks is a bit long. What type of grain are you using??
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pftek
1337


Registered: 08/18/08
Posts: 832
Last seen: 14 years, 3 months
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: T-Rex]
#10111803 - 04/05/09 05:38 PM (15 years, 9 months ago) |
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WBS quart jars.
my place is 65 degrees. And when I'm home the temps are closer to 70 degrees.
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T-Rex




Registered: 04/05/08
Posts: 4,185
Loc: NY
Last seen: 11 months, 21 days
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: pftek]
#10111978 - 04/05/09 06:01 PM (15 years, 9 months ago) |
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All i work with is wbs and have found out with wbs sterilization is one of utmost important factors. hey at least you have a few other jars.
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NineInchNails
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Registered: 03/01/09
Posts: 1,190
Last seen: 2 years, 12 days
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Re: Nothing growing on WBS jars - Resterilize or Just reinject? [Re: T-Rex]
#10112471 - 04/05/09 07:26 PM (15 years, 9 months ago) |
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Personally I think prep of the WBS is very important. I did 9 jars on my first run and all but 3 were a miserable failure. Actually even the 3 that showed life were suffering. I think the WBS was way too dry. This is how I prepared the failing jars:
1. Rinse thoroughly and chuck the floaters. Used garden hose and 5 gal bucket. 2. Soaked for 24 hrs. 3. Rinsed thoroughly again. 4. Spread out on a screen to dry in front of a fan, tumbled occasionally until it didn't stick to my hand.
ONLY 3 jars showed a TINY bit of life after 30 days. I ganked the growth and plopped it into some LC tonight. I used a glove box so I hope to preserve this strain. I don't have anything else to show for the spore syringe I used.
My next batch of jars was a 100% success.
1. Rinse thoroughly and chuck the floaters. Used garden hose and 5 gal bucket. 2. Soaked for 24 hrs. 3. Rinsed thoroughly again. 4. I boiled some water, turned OFF the flame, and tossed in the WBS. Stirred occasionally for 10 min. The WBS was saturated nearly to its core. 5. Spread out on a screen to dry in front of a fan, tumbled occasionally until it didn't stick to my hand.
Every time I've done it this way it's worked out perfectly.
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