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Voodoo486
Homie



Registered: 12/13/08
Posts: 159
Last seen: 14 years, 5 months
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1 Syringe, 24 jars
#10106094 - 04/04/09 04:43 PM (15 years, 9 months ago) |
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I planned on inoculating 24 jars from 6 syringes. I have three large prints so half a print per syringe. Well I sterilized the syringes in a pressure cooker and put the needle tips down. Now they are all bent and completely unusable. Except one.
So... Can I use 1 syringe to suck up all 3 prints and then inoculate 24 QT jars without any negative consequences?
P.S. They are 10 cc
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That voodoo that shrooms do...
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ArthurSalenez
Golden Block Of Bud



Registered: 10/23/08
Posts: 551
Last seen: 14 years, 11 months
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Re: 1 Syringe, 24 jars [Re: Voodoo486]
#10106122 - 04/04/09 04:49 PM (15 years, 9 months ago) |
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It's enough for 10 jars, 1 cc for each jar (four holes: 1 hole = 1/4 cc) But I guess you can try to squirt as little as possible. Don't forget to shake the syringe.
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init
Golden Student



Registered: 02/11/09
Posts: 151
Loc: west coast
Last seen: 13 years, 5 months
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You sure you want to use 3 whole spore prints? That seems like overkill to me. I can't imagine trying to make 10cc of spore solution last for 24 jars, but maybe that's just me. I'd feel better using the syringe as normal for the first 10-12 jars and then "refilling" it. At any rate, good luck.
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Demonic_Chronic
The Plague Doctress



Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: 1 Syringe, 24 jars [Re: init]
#10106244 - 04/04/09 05:19 PM (15 years, 9 months ago) |
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i use like 1/20th of a spore print for 1 syringe. theyre will be hundreds of thousands of spores in the syringe. the more spores you put in the more colonies you form and you have less of a uniform mycelial colony and the myc will not be as strong in a sense. Thats why i hate MS.
DC
-------------------- The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 5 years, 6 months
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more spores isnt better.
its not live mycelium.
each spore will be its own colony.
why not use 1/2 print or less per 10cc of water.(most 10cc syringes hold 12 cc if you fill them completely.)
rinse syringe out, fill with water, PC, this time with needle facing up.
and make another syringe
repeat until you have all your cakes inoculated
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-pEaCeLoVeGoDbLeSs-
"The Downfall of Mankind; is Believing He Has Limitations."
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Voodoo486
Homie



Registered: 12/13/08
Posts: 159
Last seen: 14 years, 5 months
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Re: 1 Syringe, 24 jars [Re: init]
#10106532 - 04/04/09 06:32 PM (15 years, 9 months ago) |
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It takes a day to PC since you have to let the jars cool, and it takes a day to make a syringe since you have to let the spores re-hydrate. because of this, I can follow advice and re-use 1 syringe multiple times.
Thanks for the quick and helpful advice.
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That voodoo that shrooms do...
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 5 years, 6 months
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Re: 1 Syringe, 24 jars [Re: Voodoo486]
#10106580 - 04/04/09 06:43 PM (15 years, 9 months ago) |
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-pEaCeLoVeGoDbLeSs-
"The Downfall of Mankind; is Believing He Has Limitations."
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Shroominit
Part Time Mycologist


Registered: 11/29/08
Posts: 4,662
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Re: 1 Syringe, 24 jars [Re: just me]
#10106656 - 04/04/09 06:54 PM (15 years, 9 months ago) |
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If you make up 30cc(ml) of spore solution with 1-2 of the prints, you can take turns sucking up a syringe full of the solution and aspirating it into the jars. Effectively doing all 24 + having 6cc left over.
Just PC a jar of 30cc of water, then suck that up in a syringe (silicon/whatman injection port is best here, polyfil will probably be fine if that's all you have) aspirate it into the remaining 20cc of water. IME 1 large print is definitely enough for 3 syringes.
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Demonic_Chronic
The Plague Doctress



Registered: 08/10/08
Posts: 4,199
Loc: PNW
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i didnt say more spores are better? and each spore isnt its own colony it takes 2 spores that are compatible =D
DC
-------------------- The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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just me
Friend



Registered: 07/01/07
Posts: 9,745
Loc: IL/MO/FL/TX/HI/OR
Last seen: 5 years, 6 months
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sry i didnt use the reply button. it was to the OP.
and i just learned somethin new! thanks
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-pEaCeLoVeGoDbLeSs-
"The Downfall of Mankind; is Believing He Has Limitations."
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Demonic_Chronic
The Plague Doctress



Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: 1 Syringe, 24 jars [Re: just me]
#10108508 - 04/05/09 01:16 AM (15 years, 9 months ago) |
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oh, ok haha no prob.
DC
-------------------- The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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