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OfflineVoodoo486
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Registered: 12/13/08
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1 Syringe, 24 jars
    #10106094 - 04/04/09 04:43 PM (15 years, 9 months ago)

I planned on inoculating 24 jars from 6 syringes. I have three large prints so half a print per syringe. Well I sterilized the syringes in a pressure cooker and put the needle tips down. Now they are all bent and completely unusable. Except one.

So... Can I use 1 syringe to suck up all 3 prints and then inoculate 24 QT jars without any negative consequences?

P.S. They are 10 cc


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OfflineArthurSalenez
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Re: 1 Syringe, 24 jars [Re: Voodoo486]
    #10106122 - 04/04/09 04:49 PM (15 years, 9 months ago)

It's enough for 10 jars, 1 cc for each jar (four holes: 1 hole = 1/4 cc) But I guess you can try to squirt as little as possible. Don't forget to shake the syringe.

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Offlineinit
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Re: 1 Syringe, 24 jars [Re: ArthurSalenez]
    #10106182 - 04/04/09 05:01 PM (15 years, 9 months ago)

You sure you want to use 3 whole spore prints? That seems like overkill to me. I can't imagine trying to make 10cc of spore solution last for 24 jars, but maybe that's just me. I'd feel better using the syringe as normal for the first 10-12 jars and then "refilling" it. At any rate, good luck.


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InvisibleDemonic_Chronic
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Registered: 08/10/08
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Re: 1 Syringe, 24 jars [Re: init]
    #10106244 - 04/04/09 05:19 PM (15 years, 9 months ago)

i use like 1/20th of a spore print for 1 syringe. theyre will be hundreds of thousands of spores in the syringe. the more spores you put in the more colonies you form and you have less of a uniform mycelial colony and the myc will not be as strong in a sense. Thats why i hate MS.

DC


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The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.

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Offlinejust me
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Re: 1 Syringe, 24 jars [Re: Demonic_Chronic]
    #10106268 - 04/04/09 05:28 PM (15 years, 9 months ago)

more spores isnt better.

its not live mycelium.

each spore will be its own colony.

why not use 1/2 print or less per 10cc of water.(most 10cc syringes hold 12 cc if you fill them completely.)

rinse syringe out, fill with water, PC, this time with needle facing up.

and make another syringe

repeat until you have all your cakes inoculated


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."

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OfflineVoodoo486
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Re: 1 Syringe, 24 jars [Re: init]
    #10106532 - 04/04/09 06:32 PM (15 years, 9 months ago)

It takes a day to PC since you have to let the jars cool, and it takes a day to make a syringe since you have to let the spores re-hydrate. because of this, I can follow advice and re-use 1 syringe multiple times.

Thanks for the quick and helpful advice.


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Offlinejust me
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Re: 1 Syringe, 24 jars [Re: Voodoo486]
    #10106580 - 04/04/09 06:43 PM (15 years, 9 months ago)

:thumbup:


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."

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InvisibleShroominit
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Registered: 11/29/08
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Re: 1 Syringe, 24 jars [Re: just me]
    #10106656 - 04/04/09 06:54 PM (15 years, 9 months ago)

If you make up 30cc(ml) of spore solution with 1-2 of the prints, you can take turns sucking up a syringe full of the solution and aspirating it into the jars. Effectively doing all 24 + having 6cc left over.

Just PC a jar of 30cc of water, then suck that up in a syringe (silicon/whatman injection port is best here, polyfil will probably be fine if that's all you have) aspirate it into the remaining 20cc of water. IME 1 large print is definitely enough for 3 syringes.

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InvisibleDemonic_Chronic
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Registered: 08/10/08
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Re: 1 Syringe, 24 jars [Re: Shroominit]
    #10107585 - 04/04/09 09:53 PM (15 years, 9 months ago)

i didnt say more spores are better? and each spore isnt its own colony it takes 2 spores that are compatible =D

DC


--------------------
The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.

Extras: Filter Print Post Top
Offlinejust me
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Re: 1 Syringe, 24 jars [Re: Demonic_Chronic]
    #10107884 - 04/04/09 11:01 PM (15 years, 9 months ago)

sry i didnt use the reply button. it was to the OP.

and i just learned somethin new! thanks :grin:


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-pEaCeLoVeGoDbLeSs-

"The Downfall of Mankind; is Believing He Has Limitations."

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InvisibleDemonic_Chronic
The Plague Doctress
Trans-female


Registered: 08/10/08
Posts: 4,199
Loc: PNW Flag
Re: 1 Syringe, 24 jars [Re: just me]
    #10108508 - 04/05/09 01:16 AM (15 years, 9 months ago)

oh, ok haha no prob.

DC


--------------------
The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.

Extras: Filter Print Post Top
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