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OfflineAnastrophy
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EMERGENCY...contams? rye jars
    #10102682 - 04/03/09 09:18 PM (15 years, 9 months ago)

Let's be thorough leave no details out.

Soaked grain for 24 hours, rinsed, brought to boil, put in jars and pc them. Had 5 quart jars, was able to get 3 of em filled ran out of soaked grain.

Heard u can bring to boil then simmer rye, did as a quick fix to fill the other 2 jars that same nite.

So the 3 that were full of soaked grain show condensation inside like a week later. The myc took off! But now there's deep hues of black in one myc colony, a black spot in the other, and the 3rd is like a grey looking myc colony and its clumped together.

Of the unsoaked grain, jar 4 hadn't colonized yet and jar 5 is colonizing nicely. Jars 4 and 5 had no condensation. I used micropore tape to cover the 4 noc holes.

I got pics, but I got a phone browser. Shoot me an email and ill send some pics.

So I'm gonna empty them, wear a painters mask, fill with rubbing alcohol and clean out the jars if they contamed and reuse. Or should I just toss em? Tell me after u see the pics.

Next time, what about a dry layer of verm in the jars on top? My brf jars still colonizing but they are contam free. Just 2 never colonized.

Let me know thanks in advance.

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OfflineFrequency
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Registered: 03/25/09
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Re: EMERGENCY...contams? rye jars [Re: Anastrophy]
    #10102721 - 04/03/09 09:27 PM (15 years, 9 months ago)

Sounds like your sterile technique could use some improvement. Your prints/syringes could have also been prepared in less than cleanly conditions. Great job on the BRF jars, sounds like they're on their way. Rye is a harsh mistress sometimes. It sounds like you may have cooked your rye instead of just rinsing it. It needs to be moist, not wet, when you're done PC'ing.


--------------------
McKenna: I think that I am always, from the very first psychedelic experience, had the uncanny intuition that "Yes this has been around for 50,000 years" but it somehow going to be critically important in our lifetime. That we will need it for something. Maybe just to think our way out of the mess that we're getting into.
Shulgin: If it's needed, we have it. That's the beauty. The tool is there. For the time when the need is obvious. And this may be our lifetime...

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OfflineAnastrophy
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Re: EMERGENCY...contams? rye jars [Re: Frequency]
    #10102752 - 04/03/09 09:38 PM (15 years, 9 months ago)

i didnt know what i was doing exactly with rye, now im sure the excess moisture caused an environment to invite mold. throw em out or clean the jars? lysol and oust are my friends. im pretty sure it was sterile, im gonna blame the excessive moisture as those 3 jars only had the blackness but the one jar has like grey myc? cobweb mold? i dunno. the jar has loose grain but the colonized part is clumped together

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OfflineFrequency
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Re: EMERGENCY...contams? rye jars [Re: Anastrophy]
    #10102795 - 04/03/09 09:47 PM (15 years, 9 months ago)

Cobweb mold isn't usually clumpy, however if it's anything but bright white, chances are it's a contam. Clean the jars in a bleach solution then rinse with distilled water and dry. Reuse them! There's no sense in throwing your money away.

Excessive moisture in rye is the most common problem. If there's a clumpy area that's bright white, it's just a less rhizomorphic phenotype and will still colonize and fruit just fine. It's not ideal, but it's still viable. Hope this helps!


--------------------
McKenna: I think that I am always, from the very first psychedelic experience, had the uncanny intuition that "Yes this has been around for 50,000 years" but it somehow going to be critically important in our lifetime. That we will need it for something. Maybe just to think our way out of the mess that we're getting into.
Shulgin: If it's needed, we have it. That's the beauty. The tool is there. For the time when the need is obvious. And this may be our lifetime...

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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: EMERGENCY...contams? rye jars [Re: Frequency]
    #10103113 - 04/03/09 11:09 PM (15 years, 9 months ago)

Quote:

i didnt know what i was doing exactly with rye, now im sure the excess moisture caused an environment to invite mold. throw em out or clean the jars?




The extra moisture would slow down colonization, but not cause mold.  Mold spores cause mold.  You need to either work on sterile procedure, correct your jar filter method, get cleaner inoculant, or a combination of all.
RR


--------------------
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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineAnastrophy
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Re: EMERGENCY...contams? rye jars [Re: RogerRabbit]
    #10103292 - 04/03/09 11:54 PM (15 years, 9 months ago)

So I could safely assume there is black mold elsewhere in this condo which is where the spores came from. Had them in a closet on a shelf higher than the brf jars...did it in the kitchen with a respirator and sterile bucket and ralphsters syringes. I'm quite sure nobody here would diss ralphsters ability to make steril syringes...but still, so there's black mold elsewhere. Isn't that stuff extremely toxic? Why are the brf jars sterile? They got a layer of dry verm on top...used micropore tape on grain jars...hmm...

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OfflineAnastrophy
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Registered: 03/17/07
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Re: EMERGENCY...contams? rye jars [Re: Anastrophy]
    #10106312 - 04/04/09 05:39 PM (15 years, 9 months ago)

That was a statement posed as a question...

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