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init
Golden Student



Registered: 02/11/09
Posts: 151
Loc: west coast
Last seen: 13 years, 5 months
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Is this an in vitro pin? (pic)
#10102299 - 04/03/09 07:31 PM (15 years, 9 months ago) |
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This PF tek BRF jar just reached 100% colonization this morning, about 32 days after inoculation. This is B+ from Sporeworks in regular mouth 1/2 pint Ball jars. I just noticed this little tiny guy... do my eyes deceive me, or is this my very first pin?


The jar has been kept between 72 and 78 degrees, holes uncovered, on my dresser. I find it hard to believe that it could be getting enough fresh air to start pinning, but maybe I'm wrong.
I know early pinning is cause for concern, but the jar looks and smells fine, and it did just reach 100% colonization.
My plan was to wait a week before birthing - is this still a good idea? Should I be worried at all? I've been following the PF tek from RR's video to the T, but I didn't expect this. Comments/advice appreciated!
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Breakfast Crew
Cheerios



Registered: 01/20/09
Posts: 1,985
Last seen: 13 years, 5 months
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Re: Is this an in vitro pin? (pic) [Re: init]
#10102314 - 04/03/09 07:34 PM (15 years, 9 months ago) |
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If he can survive being in a jar, im sure 24 hours of water won't hurt him one bit. Good luck!
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shaggyeg6si
Stranger


Registered: 12/29/08
Posts: 204
Last seen: 9 years, 9 months
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Re: Is this an in vitro pin? (pic) [Re: init]
#10102315 - 04/03/09 07:34 PM (15 years, 9 months ago) |
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Looks like it!
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Alabama Slim
Chinese river witch

Registered: 06/29/08
Posts: 7,439
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Re: Is this an in vitro pin? (pic) *DELETED* [Re: init]
#10103084 - 04/03/09 11:01 PM (15 years, 9 months ago) |
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Post deleted by Alabama SlimReason for deletion: .
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 10 months
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Re: Is this an in vitro pin? (pic) [Re: Alabama Slim]
#10103101 - 04/03/09 11:06 PM (15 years, 9 months ago) |
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I'd give it at least a few more days before birthing and dunk and roll. You don't really want your pins to come one at a time. Let the mycelium consolidate its hold on the substrate and you'll get a much better flush. RR
-------------------- Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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SuchSmartMonkeys
mycologically driven individual



Registered: 10/26/05
Posts: 1,154
Last seen: 3 years, 10 months
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Re: Is this an in vitro pin? (pic) [Re: RogerRabbit]
#10103111 - 04/03/09 11:09 PM (15 years, 9 months ago) |
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Quote:
RogerRabbit said: I'd give it at least a few more days before birthing and dunk and roll. You don't really want your pins to come one at a time. Let the mycelium consolidate its hold on the substrate and you'll get a much better flush. RR
this is definitely good advice... Looks like you used a full pint sized jar. I did that myself a few times, and got pins near the top before the thing was fully colonized. If you just wait it out, your final pinset will be nicer...
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Alabama Slim
Chinese river witch

Registered: 06/29/08
Posts: 7,439
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Re: Is this an in vitro pin? (pic) *DELETED* [Re: RogerRabbit]
#10103249 - 04/03/09 11:45 PM (15 years, 9 months ago) |
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Post deleted by Alabama SlimReason for deletion: .
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madsick
whoremonger



Registered: 03/19/08
Posts: 773
Last seen: 1 year, 11 months
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Re: Is this an in vitro pin? (pic) [Re: Alabama Slim]
#10103256 - 04/03/09 11:47 PM (15 years, 9 months ago) |
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eh ill usually give it 2-3 days after no brown shows pins or not.
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Alabama Slim
Chinese river witch

Registered: 06/29/08
Posts: 7,439
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Re: Is this an in vitro pin? (pic) *DELETED* [Re: madsick]
#10103274 - 04/03/09 11:51 PM (15 years, 9 months ago) |
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Post deleted by Alabama SlimReason for deletion: .
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madsick
whoremonger



Registered: 03/19/08
Posts: 773
Last seen: 1 year, 11 months
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Re: Is this an in vitro pin? (pic) [Re: Alabama Slim]
#10103402 - 04/04/09 12:34 AM (15 years, 9 months ago) |
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all depends on everythings lookin at the time, if ur tubs full save it a little while but if ur starvin bust that fucker out and get the jar ready for more. itll be done b4 the one u birthed is.
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init
Golden Student



Registered: 02/11/09
Posts: 151
Loc: west coast
Last seen: 13 years, 5 months
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Quote:
RogerRabbit said: I'd give it at least a few more days before birthing and dunk and roll. You don't really want your pins to come one at a time. Let the mycelium consolidate its hold on the substrate and you'll get a much better flush. RR
Great, that's what I wanted to do. You've given tons of great advice on this forum and this is no exception - thank you RR 
Quote:
SuchSmartMonkeys said: this is definitely good advice... Looks like you used a full pint sized jar. I did that myself a few times, and got pins near the top before the thing was fully colonized. If you just wait it out, your final pinset will be nicer...
Actually these are half-pint jars, they just have regular mouths instead of wide, which I couldn't find unfortunately. I'm kicking myself for that now, since wide mouth jars would have finished colonizing much sooner due to the smaller surface area. So I'll hunt down some wide mouth jars, and in the meantime, I'll give this jar a couple days 
Thanks everyone!
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