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OfflinePatisotagami
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Registered: 05/15/08
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Last seen: 8 years, 1 month
Grow Log: B+ & Costa Rica
    #10101714 - 04/03/09 05:52 PM (15 years, 9 months ago)

Hello. This is my 2nd grow. My last was a test and I started with an inoculated jar my friend gave me.
If anyone sees anything I could use a pointer on, or if I am doing something I should not be (or vice versa), PLEASE let me know on this thread. Thanks, enjoy! Pictures will be coming soon.

Friday, 4/3/2009:
-Received spores in the mail from The Hawk's Eye. Thanks, Hawk :smile:
-Made a glove box (per Let's Grow Mushrooms video 2) and filled 12 half-pint canning jars with a mixture of Brown Rice Flour (BRF), Vermiculite, and water. Used Let's Grow's recipe of 1 cup BRF: 2 cups Verm : 1 cup H2O.
-Labeled and Inoculated 6 half-pint jars with approx. 1.5cc of spores each using Costa Rica.
-Used 3M micropore tape to cover all 4 injection point holes on each jar. I left one jar with 2 open to see if the air exchange is better. I left yet another jar with only 1 hole open.
-Repeated with 6 jars of B+ strain. Covered ALL holes on ALL jars with B+.
-Made a light-proof heat containing box by covering all sides of a rubbermaid storage box with aluminum foil. I then covered the bottom with a heating pad I bought at Walgreens for cheap. Then I inserted the cardboard that the jars came in on top of the heating pad. The thermometer reads 65 degrees F currently, but I just turned it on so I will wait to see if it goes up. If not, I will change the heat pad from Low to Medium heat. Possibly will also add a timer to it.

Side Project: I am about to make 3 Liquid Cultures (LC's) of leftover B+, Golden Teacher, and Puerto Rico strains. Will post pictures here of those as well... Currently I am only set up to grow 2 strains, but I will try my best to get a pot or something I will be able to try growing one of my LC's in.

Monday 5/6/2009:
-Visible mycelium colonization has begun in 4 out of 6 jars of B+. None of the 6 CR jars have any signs of myc growth.
-Both are being maintained at a temperature of approximately 78-84 degrees F.

LC UPDATE:
-One of the baby bottles in which I am attempting an LC of the B+ strain had a loose cap and was leaking a bit. Possibly it is contaminated, but there is the possibility it is not. i'm going to keep it going and hope for the best.
-Puerto Rican & Golden Teacher LC's look good. They just have a bunch of little specks floating around... though the specks are becoming greater and greater in number every time I go swirl them.
-The plan is to leave them be next to my heater for another couple days and see what becomes of them.


--------------------
EVERYTHING IS EVERYTHING

Edited by Patisotagami (04/06/09 06:14 PM)

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