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Green2whitethumb
Lumberjack



Registered: 02/27/09
Posts: 533
Loc: Somewhere Over the Rainbo...
Last seen: 10 years, 5 months
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Help with Casings!!! Wheres the vets &TCs
#10100096 - 04/03/09 12:08 PM (15 years, 9 months ago) |
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Alright, I am spawning some bulk sub tonight, and as far as the process goes, I think I'm ok. But as I read through different threads I keep having the same question. Sterility.
So, when I knock up jars I use a glovebox and Im very careful. In my first few attempts I knocked up 10pf-tek 1/2 pints, and 25 qts of rye. I ended up with 6 of the 25 rye qts getting a contam and none of the pf-teks. 5 were yeast, I believe the other was trich.
So, what sterility measures do you take when you do your casings? It seems like a great opportunity for a contam to float on it while you're scooping out colonized rye with a spoon. Do you just basically do the same as innoc just without the glovebox? i.e. Clean, disinfect, shower, clean clothes, etc....just the basic stuff?
It just seems so easy to get contams with all your myc and substrate just sitting out like that. Please help with some proven techniques that you use, thank you.
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Shroominit
Part Time Mycologist


Registered: 11/29/08
Posts: 4,662
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You mean when you're spawning to bulk? A casing is a layer applied to the top of the (already colonized) substrate that creates a micro-climate for fruiting.
When you spawn to bulk, keep it clean: Wash hands and arms, make sure any tubs you are using are clean (hot soapy water will do the trick) and just don't breathe on it too much or take too long to do it. Should only take a few minutes to get it prepared.
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13shrooms
Lightning Shaman



Registered: 01/01/09
Posts: 26,719
Loc: IN ETHERS TORSION FIELD
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Re: Help with Casings!!! Wheres the vets &TCs [Re: Shroominit]
#10100373 - 04/03/09 01:18 PM (15 years, 9 months ago) |
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what he said.
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shrooman420
Stranger



Registered: 07/19/08
Posts: 245
Last seen: 9 years, 3 months
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Re: Help with Casings!!! Wheres the vets &TCs [Re: Shroominit]
#10100383 - 04/03/09 01:20 PM (15 years, 9 months ago) |
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yea man..after that colonizes get you some buckets and wheat straw ..poke lots of holes and cover with micropore tape over the holes use a monotub tech and u can spawn your substrate with the buckets stacket on top each other..you don't even need a casing and the bucket serves as a terrarium most importantly watch RogerRabbits video and do it your way but stick to how he does things to
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shrooman420
Stranger



Registered: 07/19/08
Posts: 245
Last seen: 9 years, 3 months
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Re: Help with Casings!!! Wheres the vets &TCs [Re: Shroominit]
#10100399 - 04/03/09 01:24 PM (15 years, 9 months ago) |
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yea man..after that colonizes get you some buckets and wheat straw ..poke lots of holes and cover with micropore tape over the holes use a monotub tech and u can spawn your substrate with the buckets stacket on top each other..you don't even need a casing and the bucket serves as a terrarium most importantly watch RogerRabbits video and do it your way but stick to how he does things to
stretch you mycelium out for best flushes
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Green2whitethumb
Lumberjack



Registered: 02/27/09
Posts: 533
Loc: Somewhere Over the Rainbo...
Last seen: 10 years, 5 months
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Re: Help with Casings!!! Wheres the vets &TCs [Re: Shroominit]
#10100524 - 04/03/09 01:52 PM (15 years, 9 months ago) |
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Quote:
Shroominit said: You mean when you're spawning to bulk? A casing is a layer applied to the top of the (already colonized) substrate that creates a micro-climate for fruiting.
When you spawn to bulk, keep it clean: Wash hands and arms, make sure any tubs you are using are clean (hot soapy water will do the trick) and just don't breathe on it too much or take too long to do it. Should only take a few minutes to get it prepared.
Sorry I wasn't more clear. I'm talking about spawning colonized rye to a coir/verm sub. No casing. Its easy to refer to a tray with bulk sub as a "casing", even though it's not and it probably confuses the hell out of people. Sorry.
Yes, so more directly, I was wondering what steps people take as far as sterility is concerned with spawning a bulk sub. I know I'll get posts on what to do as far as a TEK and process (as I see already), What I was concerned with is how does one minimize getting contams while performing the spawning. Like I said, It just seems so EASY to get contams in this process vs say....inoculation, where you have a still air environment in the glove.
I know the basics of sterility, and Ive spawned to bulk before (homestead kit, bag of berries spawned to a bag of kowanite), but it was pretty hands-off and simple. I guess I was just looking for tips and tricks and/or specific methods used to ensure sterility in this open air process of spawning to bulk.
-------------------- Welcome to Costco.....I love you.
***All communications from this avatar are fictitious and should be treated as such.
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Green2whitethumb
Lumberjack



Registered: 02/27/09
Posts: 533
Loc: Somewhere Over the Rainbo...
Last seen: 10 years, 5 months
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Re: Help with Casings!!! Wheres the vets &TCs [Re: shrooman420]
#10100544 - 04/03/09 01:58 PM (15 years, 9 months ago) |
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Quote:
shrooman420 said: yea man..after that colonizes get you some buckets and wheat straw ..poke lots of holes and cover with micropore tape over the holes use a monotub tech and u can spawn your substrate with the buckets stacket on top each other..you don't even need a casing and the bucket serves as a terrarium most importantly watch RogerRabbits video and do it your way but stick to how he does things to
stretch you mycelium out for best flushes
Stretch out the myc??? Do you mean use a lower ratio, like 1:10 spawn to sub?
I plan on a much better ratio like 1:2 maybe 1:3. I don't see how you get a better flush using a lower ratio. It just takes much longer, and opens you up to even more contams.
BTW, if you read my post, I wasn't looking for any of this info.
-------------------- Welcome to Costco.....I love you.
***All communications from this avatar are fictitious and should be treated as such.
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Shroominit
Part Time Mycologist


Registered: 11/29/08
Posts: 4,662
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My post above was pretty much it. I mean you can use a mask and a hair net/hat/gloves and do it in a room that has previously had a HEPA running in it for a while if you want to get carried away, but really bulk subs are pasteurized to weaken the contams and then we add a ton (1:1 - 1:4 ratio) of our own mycelium and hope that it gets the upper hand and takes over. It has a good chance of working out, so no reason to be super sterile.
Yeah, definitely don't go above a 1:6 ratio. I like 1:3, good amount of expansion (more fruiting area at cheap cost per inch) and it's fast. 1:1 is always good too, for the obvious reason.
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