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Offlinehiediho87
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5htp soaked rye
    #10085324 - 04/01/09 04:31 AM (15 years, 9 months ago)

homgenized 5htp and used as "water" for brf.


these came from the cake, they were not adulterated or abused to cause this bluing, just happened when the veil's busted, and as i harvested. Wore gloves.


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Invisibleshroomzey
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Re: 5htp soaked rye [Re: hiediho87]
    #10085330 - 04/01/09 04:34 AM (15 years, 9 months ago)

Explain how you use/prepared the 5 htp?  And potency testing?


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200 years from now, we will look back and laugh at ourselves for how stupid we were.
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Find a respected member of the community and study them.  I give thanks to: RogerRabbit, agar, hyphae, Nibin, fahtster, The shroomy 1, monstermitch, FooMan, HippieChick, Blue Helix, eatyualive, mycofile, and many, many more.
Never accept mediocrity, always return the love that is given to you.

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Offlinehiediho87
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Re: 5htp soaked rye [Re: shroomzey]
    #10085334 - 04/01/09 04:38 AM (15 years, 9 months ago)

dumped capsules in heated saline solution, reacted homogenization adding 3mL of n-heptane, gassed with -hcl, and wallah. Soluble l-tryptophan, prepared pftek cake as normal.

no bioassay by myself.
colleague is running gc/ms to compare to samples taken from same mycelium transfer, just without the tryptophan.

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Invisibleshroomzey
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Re: 5htp soaked rye [Re: hiediho87]
    #10085351 - 04/01/09 04:50 AM (15 years, 9 months ago)

Please update this accordingly.  I'm curious if the solubility of the tryptophan has a large effect on the mycelium's ability to incorporate it? 

It would seem this is superior to simply adding the tryptophan to a substrate material.  We'll see I hope?


--------------------

200 years from now, we will look back and laugh at ourselves for how stupid we were.
My Glovebox
Find a respected member of the community and study them.  I give thanks to: RogerRabbit, agar, hyphae, Nibin, fahtster, The shroomy 1, monstermitch, FooMan, HippieChick, Blue Helix, eatyualive, mycofile, and many, many more.
Never accept mediocrity, always return the love that is given to you.

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Offlinehiediho87
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Re: 5htp soaked rye [Re: shroomzey]
    #10085363 - 04/01/09 04:55 AM (15 years, 9 months ago)

in about 28 hours yes.

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OfflineCarbonKing
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Re: 5htp soaked rye [Re: hiediho87]
    #10085906 - 04/01/09 08:36 AM (15 years, 9 months ago)

if this works you've got to help us all out and write a tek for this. :thumbup:


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Live a Little...

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Offlinemilkman59
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Re: 5htp soaked rye [Re: CarbonKing]
    #10093038 - 04/02/09 09:17 AM (15 years, 9 months ago)

nice cant wait to see results


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milkman does wikidzons... fresh start 10/26/09
prints sent out today sorry for delay.....10/27/09

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Offlinefluffyshapes
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Re: 5htp soaked rye [Re: milkman59]
    #10093186 - 04/02/09 09:58 AM (15 years, 9 months ago)

ok it's been 29 hours!... ?

:smile:

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OfflineStuckintheskY
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Re: 5htp soaked rye [Re: fluffyshapes]
    #10093197 - 04/02/09 10:02 AM (15 years, 9 months ago)

:sunny:


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"All that we are is the result of what we have thought. The mind is everything. What we think we become. "

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InvisibleCheeWiz
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Re: 5htp soaked rye [Re: fluffyshapes]
    #10093290 - 04/02/09 10:21 AM (15 years, 9 months ago)

Hi: It won't work, it's been tried over the years by many people using every thing from 5Htp to adding locoweed (contains DMT) to there substrate. The biochemical path way to psilocybin and psilocin doesn't allow for external supplementing along that path way to increase potency. The potency of the mushroom is very much set by its genetics and genetic engineering is the main way to increase potency.  Hipster

Edited by CheeWiz (04/02/09 10:22 AM)

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Offlinehiediho87
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Re: 5htp soaked rye [Re: CheeWiz]
    #10093632 - 04/02/09 11:36 AM (15 years, 9 months ago)

Quote:

Hipster said:
Hi: It won't work, it's been tried over the years by many people using every thing from 5Htp to adding locoweed (contains DMT) to there substrate. The biochemical path way to psilocybin and psilocin doesn't allow for external supplementing along that path way to increase potency. The potency of the mushroom is very much set by its genetics and genetic engineering is the main way to increase potency.  Hipster





actually quantitively there was a 12% increase in both psilocine and psilocybine (gcms software addes the E for latin reasons, don't ask me)

so,

it does work.

before you stop and say "it wont" please show me the lab and GC/MS machine you are using to prove you've even done work to try to prove/disprove.

I had another thread, where an idiot had to go and post bullshit as well as the followers in the thread, so i will no longer be updating, there is another website that can viewed to see end results the link is on Hardy Diagnostics website,

"Quantitive Over View of effects of l-tryptophan mainly 5htp on active specimens"

you will have to register/apply for access to the knowledge base.

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Offlinehiediho87
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Re: 5htp soaked rye [Re: hiediho87]
    #10093639 - 04/02/09 11:36 AM (15 years, 9 months ago)

i was going to bring it here freely, but i think it's best that some information be kept within those with lab access.

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Offlinepotatonet
Quantum Scientist
I'm a teapot


Registered: 11/13/08
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Re: 5htp soaked rye [Re: hiediho87]
    #10093666 - 04/02/09 11:43 AM (15 years, 9 months ago)

12% increase...

so from what to what?

you should do this test with a penis envy strain and see what the % difference is.

I bet as the potency increases your %variation decreases.

BTW if you want to run a statistical test on plants you need 9 samples per replication, 3 full replications for each sample group (HTP and non HTP).

so technically you would need to measure a sample form 27 different cakes of htp and 27 cakes of non htp to get a statistically significant value using the department of agriculture's quantitative methods.

I do this on a daily basis, it takes a LOT of work to prove statistical significance in the plant world (yes Im considering a fungi a plant in the case of testing for potency).


--------------------
In the Quechua language of Peru there are over 1000 words for "potato"

Edited by potatonet (04/02/09 10:35 PM)

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