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ShroomWhore
Stranger



Registered: 02/25/09
Posts: 81
Loc: The Land of Oz
Last seen: 11 years, 7 months
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Have I been ass raped by contam?
#10085205 - 04/01/09 03:23 AM (15 years, 9 months ago) |
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I had previously posted a threat showing everyone how happy I was with a jar I thought was going great and had just shaken. I was told there is contam in that jar. After some ignorance and disbelief on my part I did some reading and checked my jars.
I have below 7 pics from 5 of my initial 9 jars. Can you guys let me know what you thing? It's hard to take a picture through a jar so this is the best I could do.
Out of the 9 jars: 5 looked contaminated 2 haven't started at all! 2 look OK but only around 15%...so far (1 looks quite grey but I am told it will lighten up as it gets thicker)
How I got here:
WBS: -Rinsed to get the crap out of the WBS till water ran clear -Filled pot with water and skimmed floaters -Soaked for 26 hours -Boiled hard for 10 minutes -Put into strainers and tossed until cool to remove excess surface moisture. -Filled pint jars 2/3 full. Jars have 3/8 pollyfill holes in the lids -Put foil on lids and PC's in a PC that has a max of 8psi for 2 hours (I am at sea level) -Left to cool for a few hours then put on the bench and repeated for the remaining jars -Next day I used the glove box to noc all jars with 4cc syringes I made 24 hours before. -Put in incubator between 24.8C and 27.0C -Pulled jars out almost daily due to excitement of first grow 
I have since used the same method to noc up 8 more jars, 7 of them are 2 pint jars! I will not be touching them for at least another week but of course I worry about the same results.
Pic 1:

Pic 2

Pic 3

Pic 4

Pic 5

Pic 6

Pic 7 (this is the one I have since shaken)
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shroomzey
Humble Student




Registered: 05/17/08
Posts: 904
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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10085213 - 04/01/09 03:29 AM (15 years, 9 months ago) |
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I don't know the psi/time conversion ratio, but can someone add in if 8 PSI is adequate for that length of time?
Did you immediately take the foil off of the jars after removing from the PC after complete cool down(or at least self de-pressurization)?
Don't use an incubator, let the jars sit in open air, on a high shelf or something at normal room temperature.
And I have a feeling your syringes were contaminated? Since you made them, it's likely your syringe technique was at fault.
Or your spore print was contaminated.
Could be a lot of things.
I'm sorry my "good intentions" were too late. =)
But at least now, since you logged this, you can learn from your mistakes. Isn't that awesome?
*EDIT* Oh and next time, post this in the contamination forum. Good luck.
--------------------
200 years from now, we will look back and laugh at ourselves for how stupid we were.
My Glovebox
Find a respected member of the community and study them. I give thanks to: RogerRabbit, agar, hyphae, Nibin, fahtster, The shroomy 1, monstermitch, FooMan, HippieChick, Blue Helix, eatyualive, mycofile, and many, many more.
Never accept mediocrity, always return the love that is given to you.
Edited by shroomzey (04/01/09 03:30 AM)
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ShroomWhore
Stranger



Registered: 02/25/09
Posts: 81
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Re: Have I been ass raped by contam? [Re: shroomzey]
#10085250 - 04/01/09 03:48 AM (15 years, 9 months ago) |
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Thanks shroomzey. I didn't even know there was a contam forum. Until now I never had to look for one! 
I took the jars out when they were still very hot. The foil was removed immediately and the jars left on the kitchen bench.
You might be right about the print and syringe. I was pretty open and not sterile when iI made the print. The syringe I did properly though. Maybe next time I'll do it right there in the field with my alcohol soaked hands.
I noc'd 3 of the 2 pint jars (1kg) with a print I ordered from Canada and did it with the glove box, alcohol and all so it will be a good test to see if they come out good. I have 3 of those jars on the top shelf of my wardrobe wrapped in a towel. Will the towel be a problem?
Learning rocks!
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bloomersooner
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Registered: 02/22/09
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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10085263 - 04/01/09 03:56 AM (15 years, 9 months ago) |
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ive most commonly seen - 60min 15psi. My pc max at 11psi. so I did 90min successfully. so just about 2hours seems right if the same ratio was continued.
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ShroomWhore
Stranger



Registered: 02/25/09
Posts: 81
Loc: The Land of Oz
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Re: Have I been ass raped by contam? [Re: bloomersooner]
#10085339 - 04/01/09 04:41 AM (15 years, 9 months ago) |
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Once I realised I spent AU$250 on an 8PSI PC I did a lot of reading and figured 2 hours was enough. Either an 8psi PC doesn't get hot enough and I need a better one or I am PC'ing too much and popping kernels and causing contamination. I have noticed a lot of split kernels now what I think about it.
The 2 jars which haven't even budged dont show any sugeggestion of contamination so maybe it is the print that is the cause?
I would happily do a whole new batch but I dont know what to do different.
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ShroomWhore
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Registered: 02/25/09
Posts: 81
Loc: The Land of Oz
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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10085398 - 04/01/09 05:25 AM (15 years, 9 months ago) |
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Can I get this straight...are all 7 pics of pure and unquestionable contam? Do I toss all these jars?
Is a new sterile print the only suggestion for the future?
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shroomiin
ʂɧɽɸɵɱɨʝɳ



Registered: 12/24/07
Posts: 2,494
Loc: Zone 6
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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10085402 - 04/01/09 05:26 AM (15 years, 9 months ago) |
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i see some cobweb mold and some green mold.
-------------------- ʋɳʛɼɑʈɛʄʋɭɖɛɑɗ
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emstik5
+1



Registered: 02/24/09
Posts: 97
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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10085412 - 04/01/09 05:32 AM (15 years, 9 months ago) |
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I know what I am going to be spending my $900 tax stimulus thingy on :P
My 7L/12PSI is too small. Only fits 3 x 500ml jars. It was damn expensive too! There is not really a market for them around here.
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ShroomWhore
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Registered: 02/25/09
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Loc: The Land of Oz
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Re: Have I been ass raped by contam? [Re: emstik5]
#10090075 - 04/01/09 08:32 PM (15 years, 9 months ago) |
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OK, I've resolved to the fact I have contaminated spore syringes. Unfortunately I dont have any other prints left and would have to wait for weeks to order a new one from overseas. With that in mind I have had a brilliant idea and would like some feedback.
I do have some very healthy and thick myc in these jars. A few jars have a good amount of myc growing with only one or two little spots of mold. I thought I could open the jars up in a place away from any growing areas (like the garden shed) and pull out some myc. I could then soak it in H2O2 overnight to kill any spores and mold then place the myc in new jars. Would overnight be too long or not long enough?
It's just crazy enought to work!
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brainsOplenty
myconut



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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10090123 - 04/01/09 08:39 PM (15 years, 9 months ago) |
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Quote:
ShroomWhore said: OK, I've resolved to the fact I have contaminated spore syringes. Unfortunately I dont have any other prints left and would have to wait for weeks to order a new one from overseas. With that in mind I have had a brilliant idea and would like some feedback.
I do have some very healthy and thick myc in these jars. A few jars have a good amount of myc growing with only one or two little spots of mold. I thought I could open the jars up in a place away from any growing areas (like the garden shed) and pull out some myc. I could then soak it in H2O2 overnight to kill any spores and mold then place the myc in new jars. Would overnight be too long or not long enough?
It's just crazy enought to work! 
just wait til ya get a new print or syringe
-------------------- FOR GOD'S SAKE MAN! TELL ME ABOUT THE FUCKING GOLF SHOES!!!
LIVIN THE LIFE!!!
"WE KNOCK NIGGAS OUT AND MAKE EM BOUNCE LIKE RICKY HATTON"- IMMORTAL TECHNIQUE
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NineInchNails
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Registered: 03/01/09
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Re: Have I been ass raped by contam? [Re: brainsOplenty]
#10090184 - 04/01/09 08:48 PM (15 years, 9 months ago) |
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I would pick up a PC that will get you up to 15psi. A small one will work, but you'll eventually want a bigger one. Check eBay, Craig's List, etc... I've seen some great deals there.
I wouldn't open those jars up indoors or keep them if the're contaminated. Personally I just don't think it's worth the risk. You'll put weeks and weeks into it and might just end up with more contamination.
If I had a print I'd send ya one, but I'm not gonna have one for weeks to come. Get a PC and start over.
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m08b08
BrainDead



Registered: 01/14/09
Posts: 1,083
Loc: In The Fog
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Re: Have I been ass raped by contam? [Re: NineInchNails]
#10090197 - 04/01/09 08:51 PM (15 years, 9 months ago) |
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yea get a pressure cooker !
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: Have I been ass raped by contam? [Re: m08b08]
#10090230 - 04/01/09 08:56 PM (15 years, 9 months ago) |
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Quote:
m08b08 said: Get a pressure cooker !
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m08b08
BrainDead



Registered: 01/14/09
Posts: 1,083
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Re: Have I been ass raped by contam? [Re: Blutjager]
#10090239 - 04/01/09 08:57 PM (15 years, 9 months ago) |
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ShroomWhore
Stranger



Registered: 02/25/09
Posts: 81
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Re: Have I been ass raped by contam? [Re: Blutjager]
#10090298 - 04/01/09 09:04 PM (15 years, 9 months ago) |
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I paid $250 for this one! I understand if something is not working though.
I do have a jar that is almost 3 weeks old and put in the same conditions as the others that didn't get nocc'd and it is perfectly healthy. Doesn't that mean he PC'ing is working OK? Surely if it wasn't it would have had mold after a few days?
That reminds me, as an experiment I nocc'd a jar without PC'ing it and it was messed up with mold within a few days.
It has to be the syringe, right?
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10090493 - 04/01/09 09:32 PM (15 years, 9 months ago) |
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Either a bad syringe or bad sterile work,I went from I swear like a 90% contamination rate to next to no contamination with doing nothing different but doing all my inoculations in still air and using diluted bleach and 70% isopropyl alcohol to spray everything down... O and micopore tape sprayed with alcohol,I swear I used to lose almost EVERYTHING to Trich
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bloomersooner
Stranger


Registered: 02/22/09
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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10090513 - 04/01/09 09:34 PM (15 years, 9 months ago) |
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i guess since you have to wait for spores. I would take a tiny amount of the healthiest mycelium you can find and stick it in a bottle LC. just for kicks *shrug*
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
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Loc: Seattle
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Re: Have I been ass raped by contam? [Re: ShroomWhore]
#10090529 - 04/01/09 09:37 PM (15 years, 9 months ago) |
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This thread has been closed.
Reason:
Quote:
Have I been ass raped by contam?
I sincerely hope you haven't injected contents of said jars rectally.
All are contaminated, and you are correct; it's your inoculant. Advice to get a new PC may be sound, but it isn't the problem with this grow. Those are molds, and mold spores die at a temperature way below boiling, so the PC isn't the problem.
This is off topic here and I see you posted a duplicate thread in the contamination forum, so I'll go ahead and close this one. RR
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