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OfflineHarlz
Stranger

Registered: 01/29/09
Posts: 624
Last seen: 15 years, 1 month
am i doomed? my inoculation procedure
    #10088833 - 04/01/09 05:48 PM (15 years, 9 months ago)

i heated the tipe of the syringe and shot a little solution out and then injected all the jars without flame sterilizing between, also i forgot to shake the needle is this a really big deal? I used all 10 cc in only 6 jars so i had extra also any possible ways of contam i washed my hands wiht rubbing alcohol then de taped the holes then put on latex gloves for inoculation. they are currently untaped with a dry vermiculite layer on top also i think some water entered a jar while steam sterilizing  im doing brf cakes so should i be good? my incubater is like 82-75 degrees ranging help please:)


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InvisiblePhraggle
Psilocybe Therapy Advocate


Registered: 04/19/06
Posts: 161
Re: am i doomed? my inoculation procedure [Re: Harlz]
    #10088906 - 04/01/09 05:57 PM (15 years, 9 months ago)

Not doomed. I never flame sterilize and have never had problems. I always thought it was over kill. I just wipe the needle with alcohol before injection. I don't even wear gloves since my fingers never touch the needle. Just wait and see what happens.


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OfflineHarlz
Stranger

Registered: 01/29/09
Posts: 624
Last seen: 15 years, 1 month
Re: am i doomed? my inoculation procedure [Re: Phraggle]
    #10089664 - 04/01/09 07:39 PM (15 years, 9 months ago)

someone pleas egive me expert advice im concerned!
will they be ok im got them at like 82 right now under a towel in a rubbermaid


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InvisibleMonkee
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Registered: 07/21/08
Posts: 596
Re: am i doomed? my inoculation procedure [Re: Harlz]
    #10089673 - 04/01/09 07:41 PM (15 years, 9 months ago)

shaking the needle may be a problem if it was sitting for a long while

but it doesn't sound terrible, as along as you were sterile i wouldn't worry till you see mold

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OfflineOttoGenerated
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Registered: 02/23/09
Posts: 309
Last seen: 13 years, 2 months
Re: am i doomed? my inoculation procedure [Re: Monkee]
    #10090113 - 04/01/09 08:38 PM (15 years, 9 months ago)

82 is too hot. Room temp is best.

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OfflineSillyBilly
Professional


Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 9 years, 29 days
Re: am i doomed? my inoculation procedure [Re: OttoGenerated]
    #10090140 - 04/01/09 08:41 PM (15 years, 9 months ago)

shaking the needle distributes that large cloud of spores so its not all in one clump. since u didnt shake u may have ended up with a cpl jars getting most of the spores


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    You're a better man than I am, Gunga Din

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Offlinefishy
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Registered: 03/08/09
Posts: 158
Last seen: 10 years, 4 months
Re: am i doomed? my inoculation procedure [Re: SillyBilly]
    #10090211 - 04/01/09 08:53 PM (15 years, 9 months ago)

should be fine, wait and see is really the only way to check.


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OfflineHarlz
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Registered: 01/29/09
Posts: 624
Last seen: 15 years, 1 month
Re: am i doomed? my inoculation procedure [Re: SillyBilly]
    #10090218 - 04/01/09 08:54 PM (15 years, 9 months ago)

fuck eh... well it was 10 cc to 6 jars(1/2 pint) so would it be safe to assume they all got some? also the needle was heated till red hot and then all jars were inoculated while on an oven rack at 150 but pulled out of course with gloves on and the lids were wiped with rubbing alcohol swabs after wards should i be good?:P


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Edited by Harlz (04/01/09 08:55 PM)

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OfflineNineInchNails
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Registered: 03/01/09
Posts: 1,190
Last seen: 2 years, 12 days
Re: am i doomed? my inoculation procedure [Re: SillyBilly]
    #10090223 - 04/01/09 08:56 PM (15 years, 9 months ago)

Try not to worry so much. Easier said than done ... I know. Next time you'll do much better. I'd keep these jars around for at least a month. I still have 7-9 of my first jars in the incubator from a month ago and they still show no signs. I'll reinoculat them soon.

One day soon you'll have a ton of jars full of pearly white.

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OfflineHarlz
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Registered: 01/29/09
Posts: 624
Last seen: 15 years, 1 month
Re: am i doomed? my inoculation procedure [Re: Harlz]
    #10090242 - 04/01/09 08:57 PM (15 years, 9 months ago)

hopefully soon my jars will be full of pearly white :wink:


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OfflineSillyBilly
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Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
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Re: am i doomed? my inoculation procedure [Re: Harlz]
    #10090484 - 04/01/09 09:30 PM (15 years, 9 months ago)

NEVER USE THE OVEN TEK

not yelling at you but it should be made clear
RR has stated its a very bad idea and should be removed as a tek
everything else you have done well


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By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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OfflineJPmagic
Welcome to the Dark Side
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Registered: 03/02/09
Posts: 455
Loc: Midwest, US
Last seen: 9 years, 4 months
Re: am i doomed? my inoculation procedure [Re: Harlz]
    #10090497 - 04/01/09 09:32 PM (15 years, 9 months ago)

Well you're not doomed yet I guess.  Flame sterilization is just an extra precaution for contaminates.  Also, the reason you should shake the syringe is to spread the spores throughout the solution, so depending on how long the syringe was sitting, some of your jars may not of gotten inoculated.  Although it isn't for sure.  Only time will tell by checking your jars closely for contaminations and mycelium.  Don't lose hope yet.  Things could still go ok.


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Offlinegotfungus
get some

Registered: 11/03/08
Posts: 501
Last seen: 10 years, 4 months
Re: am i doomed? my inoculation procedure [Re: JPmagic]
    #10090517 - 04/01/09 09:35 PM (15 years, 9 months ago)

all inoculation work should be done in a glove box for best results.


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OfflineHarlz
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Registered: 01/29/09
Posts: 624
Last seen: 15 years, 1 month
Re: am i doomed? my inoculation procedure [Re: gotfungus]
    #10090539 - 04/01/09 09:39 PM (15 years, 9 months ago)

thanks for the help fuck i regret the oven thing damn :frown: I may need another 60-70$ to redo this project then fuckk but i have hope in the midst of everything going wrong these little shrooms will be the rebirth aha sorry im stoned but thanks for the help ill post pics when i got white or something else


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OfflineSillyBilly
Professional


Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
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Re: am i doomed? my inoculation procedure [Re: Harlz]
    #10090628 - 04/01/09 09:55 PM (15 years, 9 months ago)

1. dont throw it out, you havnt told us how long its been.
2. it shouldnt cost u more then 40 at most to get that set up
  you can get 2 syringes of their choice from sporeworks for like 15 with shipping, a tub costs like 10$ at big lots, some latex gloves, and maybe some more jars if you need them. i assume you didnt use up all of your substrate material.

making a flame like they show in the vids is very simple. you just take a regular half pint jar, punch a hole in its lid with a regular sized nail or something that size. pour some regular isopropyl alcohol in it so its about half way full. i use 91% btw. take a paper towel and cut it to about 5 or 6 inches maybe smaller what ever fits in the jar and roll it up pretty tight. let it soak in the alcohol and then pull it through the hole in the lid, i like to kind of get a cork screw thing going and twist it through so it stays in a rolled form. only pull out about half an inch or less through the hole at time. put the lid on with the short wick on the outside and the rest of it just sitting in the alcohol inside the jar and close the jar so its almost air tight. if the wick isnt pretty wet or alcohol doesnt come out if u squeeze it pull out more and cut off the dry part. usually a good idea to do that with each use. always make sure there is plenty of alcohol in the jar. when you light it up it shouldnt burn the wick too much or at all. probably the first time u light it it might burn a little but its probably cuz it wasnt thoroughly soaked. when its time to put it out i use a metal measuring cup and snuff it out(size 1cup). i think ive explained it pretty well and it should be completely safe. it works by the wick pulling up alcohol to the top to burn instead of the paper through a sort of vacuum. dont for any reason open the jar while it is lit, i dont know why you would but its just a precaution.


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By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

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OfflineHarlz
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Registered: 01/29/09
Posts: 624
Last seen: 15 years, 1 month
Re: am i doomed? my inoculation procedure [Re: SillyBilly]
    #10092635 - 04/02/09 07:04 AM (15 years, 9 months ago)

It has been a day today I inocculated last night and ill check them tonight for any possible mycellia?  I did everything by the guide proper substrate mixture etc but i will be using all my vermiculite to complete my terrarium with a wet vermiculite base and the spore syringes here are 50$ a piece and I dont have a credit card so it makes things more challenging but i will keep trying until i get a successful flush


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Offlinepuckmonster
The Loooooone Stranger
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Registered: 02/07/09
Posts: 63
Loc: Central Texas
Last seen: 3 years, 10 months
Re: am i doomed? my inoculation procedure [Re: Harlz]
    #10092655 - 04/02/09 07:14 AM (15 years, 9 months ago)

naa you wont see anything tonight even with lc it takes several days and spores take like 7 days to germ then a couple days later you will see little white bits.

Also get that temp down 82 is too hot put them on a shelf at room temp

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OfflineHarlz
Stranger

Registered: 01/29/09
Posts: 624
Last seen: 15 years, 1 month
Re: am i doomed? my inoculation procedure [Re: puckmonster]
    #10092675 - 04/02/09 07:21 AM (15 years, 9 months ago)

Is 82 too hot or what? The tek says otherwise i need help i heard 86 is the optimum temperature for mycellial growth


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Offlinepuckmonster
The Loooooone Stranger
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Registered: 02/07/09
Posts: 63
Loc: Central Texas
Last seen: 3 years, 10 months
Re: am i doomed? my inoculation procedure [Re: Harlz]
    #10092678 - 04/02/09 07:23 AM (15 years, 9 months ago)

yeah that sounds like old info do a search for incubation temps if you don't believe me ^_^

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OfflineOttoGenerated
Stranger

Registered: 02/23/09
Posts: 309
Last seen: 13 years, 2 months
Re: am i doomed? my inoculation procedure [Re: puckmonster]
    #10094323 - 04/02/09 01:35 PM (15 years, 9 months ago)

Quote:

puckmonster said:
naa you wont see anything tonight even with lc it takes several days and spores take like 7 days to germ then a couple days later you will see little white bits.

Also get that temp down 82 is too hot put them on a shelf at room temp




Some people have seen spore germination in only a couple/few days, although he should wait at least a couple of weeks before he gives up on this batch (not that I'm saying it won't work).

Good luck, Harlz, though you won't need it since you followed the procedures correctly.

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Shop: North Spore Bulk Substrate   Mushroom-Hut Grow Bags   Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   MagicBag.co All-In-One Bags That Don't Suck   Myyco.com Isolated Cubensis Liquid Culture For Sale


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