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Akira4321
HNIC



Registered: 03/13/02
Posts: 246
Loc: U.S.
Last seen: 4 years, 7 months
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Using Oust Or Lysol In Glovebox
#10073242 - 03/30/09 10:58 AM (14 years, 10 months ago) |
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When I'm using my glovebox do I need to spray lysol or oust before I do any innoculating or grain transfers?
-------------------- Product Of Genetic Darkness
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FractalXplora
Grainiack




Registered: 02/11/06
Posts: 2,494
Loc: UK
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Re: Using Oust Or Lysol In Glovebox [Re: Akira4321]
#10073252 - 03/30/09 11:00 AM (14 years, 10 months ago) |
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yes,put all your bits in the box, spray it good, wait 10-15 minutes and get busy.
I also wipe the inside box down with alchol then spray, to be sure.
If oyur using a flame that really should be outside the box for safety.
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Green2whitethumb
Lumberjack



Registered: 02/27/09
Posts: 533
Loc: Somewhere Over the Rainbo...
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Re: Using Oust Or Lysol In Glovebox [Re: FractalXplora]
#10073352 - 03/30/09 11:31 AM (14 years, 10 months ago) |
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I put everything I'm going to use in a room, all laid out. I then spray the room with oust (I fog it up). I leave the room, and get myself prepared. I Shower, put on clean clothes, a hairnet (clean beenie or hat works), brush teeth, dust mask, and generously rub alcohol on my hands and arms, put on latex gloves, and rub alcohol again.
Then I go back in the room (make sure the room is draft-free, and turn off the central heat/AC before you even sprayed the oust) and I spray the glovebox with 91% isopropyl in side and out, rub it down good w/ paper towel, then with an alcohol soaked paper towel I rub everything down one by one as it goes in the glove. The grain jars, any tools, syringes, everything. Then I get comfy, light up the lamp to flame sterilize the needle (which I do between every jar) then I wrap the needle in a alcohol soaked piece of paper towel until it's in the glove about to be inserted in a jar.
I am a noob, but I'd rather take the extra sterility precautions than be lax about it. I've knocked up 35 jars this way (25 rye qts and 10 pf tek), between 3 innoc sessions.
The only contam issues I've had is a yeast contam in 4 jars of grain. I use 1 syringe per 4 qt jars, the contam was all the same strain, so it had something to do with that one syringe. Either contamed, or more likely, something I did in handling it.
Good luck.
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***All communications from this avatar are fictitious and should be treated as such.
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AngryPhil
Herenow

Registered: 01/22/09
Posts: 1,201
Last seen: 10 years, 4 months
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lysol works great. i use my walk in shower as a walk in glove box and spray the crap out of it with lysol before and during anything i do in there. when i do g2g i can leave uncolonized grain exposed to the air for minutes at a time and i have never gotten a contam with this method and i'm sure lysol would be equally effective in a glove box. if you don't disinfect the air inside your glove box, then there's no reason to use a glove box at all.
-------------------- The creation takes hand of the chisel...
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teamfdn
Invitro Explorer


Registered: 12/30/08
Posts: 370
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Re: Using Oust Or Lysol In Glovebox [Re: AngryPhil]
#10073465 - 03/30/09 11:55 AM (14 years, 10 months ago) |
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I have always flamed right inside the box.
After I wipe down the glovebox with alcohol, and I spray oust in there and let it settle for about 15 minutes.
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AngryPhil
Herenow

Registered: 01/22/09
Posts: 1,201
Last seen: 10 years, 4 months
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Re: Using Oust Or Lysol In Glovebox [Re: teamfdn]
#10073495 - 03/30/09 12:00 PM (14 years, 10 months ago) |
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Quote:
teamfdn said: I have always flamed right inside the box.
After I wipe down the glovebox with alcohol, and I spray oust in there and let it settle for about 15 minutes.
hey, that was your 100th post. congratulations
-------------------- The creation takes hand of the chisel...
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NineInchNails
Stranger

Registered: 03/01/09 
Posts: 1,190
Last seen: 1 year, 1 month
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Re: Using Oust Or Lysol In Glovebox [Re: teamfdn]
#10073511 - 03/30/09 12:06 PM (14 years, 10 months ago) |
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I typically spray the GB with lysol and wipe.
I place everything I need inside. I spray again.
I typically flame sterilize outside the GB because I use an alcohol lamp. I then wipe the needle using 70% alcohol while inside the GB.
Anything you do will help battle contam. Some methods are more effective than others, but just do whatever has worked for you. If it makes sense to change something that you've been doing in the past then great.
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Captain Caveman
Uh-oh!



Registered: 01/12/09
Posts: 2,253
Last seen: 2 months, 7 days
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Re: Using Oust Or Lysol In Glovebox [Re: teamfdn]
#10073626 - 03/30/09 12:27 PM (14 years, 10 months ago) |
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Quote:
teamfdn said: I have always flamed right inside the box.
After I wipe down the glovebox with alcohol, and I spray oust in there and let it settle for about 15 minutes.
It is not smart to do that. If you dont know why, or just happened to miss the big boom days, do a search on boom and glovebox. Technically, you dont need to worry about spraying shit in there. Just clean it with bleach or otherwise if using a open flame in there. The whole point of a glovebox is to be able to work in a draft free space.
-------------------- "Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"
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teamfdn
Invitro Explorer


Registered: 12/30/08
Posts: 370
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my results suggest otherwise.
i do use open armholes though, so fume dissipation isn't much of an issue.
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Captain Caveman
Uh-oh!



Registered: 01/12/09
Posts: 2,253
Last seen: 2 months, 7 days
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Re: Using Oust Or Lysol In Glovebox [Re: teamfdn]
#10074061 - 03/30/09 01:51 PM (14 years, 10 months ago) |
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Like I said, just do a search on glovebox boom. Heres one..... http://www.shroomery.org/forums/showflat.php/Number/7759997#7759997 There are MANY out there that have burned the hair off their arms or worse using oust and alchohol in the box.
-------------------- "Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 12 days
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Never use lysol or oust in the glovebox. It isn't necessary. It's best to flame your tools and needles outside the box and then immediately put them inside to use. I'd suggest setting up your glovebox as shown in this video. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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teamfdn
Invitro Explorer


Registered: 12/30/08
Posts: 370
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Re: Using Oust Or Lysol In Glovebox [Re: RogerRabbit]
#10074128 - 03/30/09 02:08 PM (14 years, 10 months ago) |
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like I said caveman, it's never been a problem. 15 minutes is beyond adequate time for fumes to dissipate, especially in a non airtight container.
i do what works for me, you do what works for you. at the end of the day, my experiments are a success and that's all that matters.
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AngryPhil
Herenow

Registered: 01/22/09
Posts: 1,201
Last seen: 10 years, 4 months
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Re: Using Oust Or Lysol In Glovebox [Re: RogerRabbit]
#10074259 - 03/30/09 02:33 PM (14 years, 10 months ago) |
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Quote:
RogerRabbit said: Never use lysol or oust in the glovebox. It isn't necessary. It's best to flame your tools and needles outside the box and then immediately put them inside to use. I'd suggest setting up your glovebox as shown in this video. RR
what about the risk of airborne contams? can't bacteria, mold spores, etc still be floating around in the air inside the glove box waiting to land on some exposed grain, or will the lack of moving air cause them to settle to the bottom? i always assume it's just better to be safe. could lysol or oust hurt anything?
-------------------- The creation takes hand of the chisel...
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Captain Caveman
Uh-oh!



Registered: 01/12/09
Posts: 2,253
Last seen: 2 months, 7 days
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Re: Using Oust Or Lysol In Glovebox [Re: teamfdn]
#10074407 - 03/30/09 02:58 PM (14 years, 10 months ago) |
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Quote:
teamfdn said: like I said caveman, it's never been a problem. 15 minutes is beyond adequate time for fumes to dissipate, especially in a non airtight container.
i do what works for me, you do what works for you. at the end of the day, my experiments are a success and that's all that matters.
I never doubted that it worked. Im not trying to rub you the wrong way, I just dont want another shroomerite blowin their shit up, lol.
-------------------- "Captain CAAAAAAAVEMAAAAAAAAANNNN!!!!"
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Green2whitethumb
Lumberjack



Registered: 02/27/09
Posts: 533
Loc: Somewhere Over the Rainbo...
Last seen: 9 years, 7 months
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Quote:
Captain Caveman said:
Quote:
teamfdn said: like I said caveman, it's never been a problem. 15 minutes is beyond adequate time for fumes to dissipate, especially in a non airtight container.
i do what works for me, you do what works for you. at the end of the day, my experiments are a success and that's all that matters.
I never doubted that it worked. Im not trying to rub you the wrong way, I just dont want another shroomerite blowin their shit up, lol.
-------------------- Welcome to Costco.....I love you.
***All communications from this avatar are fictitious and should be treated as such.
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teamfdn
Invitro Explorer


Registered: 12/30/08
Posts: 370
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no worries.
don't get me wrong, i can definitely see how easily it can happen.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 12 days
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Re: Using Oust Or Lysol In Glovebox [Re: AngryPhil]
#10074929 - 03/30/09 04:29 PM (14 years, 10 months ago) |
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Quote:
AngryPhil said:
what about the risk of airborne contams? can't bacteria, mold spores, etc still be floating around in the air inside the glove box waiting to land on some exposed grain, or will the lack of moving air cause them to settle to the bottom? i always assume it's just better to be safe. could lysol or oust hurt anything?
If you've made a still air glovebox, nothing will be floating in the air, because the air is still. In addition, if you're worried, spray the inside walls and floor/ceiling with plain water so anything floating will stick. Yes, Lysol can hurt you if you get a flame too close, as many growers have found out. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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DZ
ranger



Registered: 01/25/09
Posts: 381
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Re: Using Oust Or Lysol In Glovebox [Re: RogerRabbit]
#10075322 - 03/30/09 05:27 PM (14 years, 10 months ago) |
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I put my glovebox on the counter, open it and put in my jars and whatever equipment I'm using, close it and spray the area outside the glove box with oust for 5-10 seconds and then inside the each glove hole for less than a second. I wait about 5-10 minutes, then I wash my hands in the sink next to my glovebox, and I'm ready to roll.
I don't do the shower right before, fresh clothes thing, I just wash and go; however, I do try to be as efficient as possible with my motion. This has worked for me many times. Good luck
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