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PreparationH
apply daily


Registered: 03/28/05
Posts: 18,413
Loc: Amsterdam
Last seen: 12 hours, 21 minutes
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what does this mean, if anything?
#10074222 - 03/30/09 02:26 PM (15 years, 9 months ago) |
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20 casings in the fruiting chamber for one week today. One of the casings grew two shrooms about 3 inches a peice while the others still haven't shown any pins. I have read that this may be a sign of contamination and it's making itself fruit.
So is this an early fruiter out of the 20 and should i clone these fruits, or is the contam a possibility?
Anyone?
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demonicaeroponic
Ghost


Registered: 07/20/08
Posts: 276
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Re: what does this mean, if anything? [Re: PreparationH]
#10074400 - 03/30/09 02:57 PM (15 years, 9 months ago) |
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clone the bad bitch.
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Duggstar



Registered: 01/20/09
Posts: 6,273
Loc: Ireland
Last seen: 2 years, 1 month
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I'm no expert on cloning, and to be honest I've never done it. But I believe you can isolate a clone from a contam by transferring it from petri dish to petri dish a number of times until you get a pure strain.
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Shroominit
Part Time Mycologist


Registered: 11/29/08
Posts: 4,662
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Re: what does this mean, if anything? [Re: Duggstar]
#10074537 - 03/30/09 03:19 PM (15 years, 9 months ago) |
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If you're careful when cloning: Glove box, clean conditions, flamed tools, alcohol swab for the stem, etc... You will not need to clean it up on agar.
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PreparationH
apply daily


Registered: 03/28/05
Posts: 18,413
Loc: Amsterdam
Last seen: 12 hours, 21 minutes
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i'm not worried much about the process of cloning it as much as whether or not these are worth it or not. My main question is more about why it pinned so early? I want a TC's opinion truthfully or someone who has shown me they're no noob.
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Shroominit
Part Time Mycologist


Registered: 11/29/08
Posts: 4,662
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Re: what does this mean, if anything? [Re: PreparationH]
#10074616 - 03/30/09 03:34 PM (15 years, 9 months ago) |
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I know this doesn't help you, but I have an HE isolate that I can not keep from pinning early. I mean it will fruit on agar, grains, anything I put it on, without warning. In my opinion, that is genetic for sure. I wouldn't count on one cake to prove that quality though.
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PreparationH
apply daily


Registered: 03/28/05
Posts: 18,413
Loc: Amsterdam
Last seen: 12 hours, 21 minutes
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Re: what does this mean, if anything? [Re: Shroominit]
#10074747 - 03/30/09 04:01 PM (15 years, 9 months ago) |
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well it's a shame because I can't clone the mushrooms Im about 250 miles away from the setup. Just for future references i made this thread.
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PreparationH
apply daily


Registered: 03/28/05
Posts: 18,413
Loc: Amsterdam
Last seen: 12 hours, 21 minutes
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Re: what does this mean, if anything? [Re: PreparationH]
#10077126 - 03/30/09 09:42 PM (15 years, 9 months ago) |
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bump
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PreparationH
apply daily


Registered: 03/28/05
Posts: 18,413
Loc: Amsterdam
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Re: what does this mean, if anything? [Re: PreparationH]
#10087301 - 04/01/09 01:46 PM (15 years, 9 months ago) |
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bump. **Original Question**
One of the casings grew two shrooms about 3 inches a peice while the others still haven't shown any pins. I have read that this may be a sign of contamination and it's making itself fruit.
So is this an early fruiter out of the 20 and should i clone these fruits, or is the contam a possibility?
Also, now 5 more started pinning with I'd say 50 pins per tray (6qt tray) everything looks good but i have lost 2 more trays to triche, im down to 18 now so no worries pic soon.
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PreparationH
apply daily


Registered: 03/28/05
Posts: 18,413
Loc: Amsterdam
Last seen: 12 hours, 21 minutes
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Re: what does this mean, if anything? [Re: PreparationH]
#10094315 - 04/02/09 01:34 PM (15 years, 9 months ago) |
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**Original Question**
One of the casings grew two shrooms about 3 inches a peice while the others still haven't shown any pins. I have read that this may be a sign of contamination and it's making itself fruit.
So is this an early fruiter out of the 20 and should i clone these fruits, or is the contam a possibility?
Also, now 5 more trays started pinning with I'd say 50 pins per tray (6qt trays) everything looks good but i have lost 2 more trays to triche, im down to 18 now so no worries pic soon.
I'm going to keep bumping until I get SOME feedback on my questions
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Duggstar



Registered: 01/20/09
Posts: 6,273
Loc: Ireland
Last seen: 2 years, 1 month
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Re: what does this mean, if anything? [Re: PreparationH]
#10103857 - 04/04/09 04:58 AM (15 years, 9 months ago) |
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I think nobody has answered your question because you don't have any pics, and its kind of hard to identify contamination without any pics. Anyway, I found this interesting thread on identifying contamination. Hope it helps. http://www.shroomery.org/forums/showflat.php/Number/620791#620791
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