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OfflineTrippyJuice
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Registered: 03/10/09
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first signs of growth? PICS
    #10067798 - 03/29/09 04:20 PM (15 years, 9 months ago)

I inoculated 5 days ago. At the Inoculation points it looks wet, oily, but no discoloration in some of my jars. In other jars I am starting to see white spots about the size of a small pea.

Is this normal for the inoculation points to look wet and oily before they start to turn white?

I'm just trying to learn what to look for as the jars progress. Is there any way to tell if jars are contaminated and or colonizing this early?

I inoculated with a spore cube cambo syringe into brf/verm cake. This is my first grow... I want to know what to look for as the process furthers thanks!



good sign bad sign etc?


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We trippy mane!

Edited by TrippyJuice (03/29/09 05:36 PM)

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OfflineEnothe
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Re: first signs of growth? PICS [Re: TrippyJuice]
    #10068537 - 03/29/09 06:00 PM (15 years, 9 months ago)

usually i dont get any signs of growth until a week with spores, and even then only small patches at the innoc points. like this!


is the growth the slighly brown spot? looks kinda like a contam... but it could just be real wet. give it some time, just make sure it doesnt turn any pretty colors, you want snow white only.


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"Never Knows Best"

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OfflineTrippyJuice
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Re: first signs of growth? PICS [Re: Enothe]
    #10068603 - 03/29/09 06:11 PM (15 years, 9 months ago)

Yea it just looks wet no colorization yet! what do you innoc points look like before it turns white?


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We trippy mane!

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OfflineEnothe
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Re: first signs of growth? PICS [Re: Enothe]
    #10068716 - 03/29/09 06:27 PM (15 years, 9 months ago)

usually doesn't look like anything till the white pops up.
sometimes they get that soggy look if u used too much water but thats usually is near the bottom.

did you use alot of the spore solution for each spot? and was it from a trusted vendor(known clean)? its probably just the solution soaked in there.

i wouldnt worry too much unless it gets darker or colorful, but i know it can seem like forever to colonize on your first attempt. and sometimes spores can be lazy, it took 2 weeks for one syringe i got to show anything.


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"Never Knows Best"

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OfflineTrippyJuice
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Re: first signs of growth? PICS [Re: Enothe]
    #10068797 - 03/29/09 06:38 PM (15 years, 9 months ago)

Yea i know I'm getting ahead of things I just like to know what to look for Since I'm still a nub :mad:!

On some of the jars I think i did inoculate a little to much maybe close to 1cc or a little less in 1 or two of the inoculation points! Vendor was from Ralphster I've heard good thing about him!

I have a little bit of spores left and I'm going to try to make some liquid culture.. F*** waiting lol, hopefully I'll be successful! thanks for your thoughts though I really appreciate it.


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We trippy mane!

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OfflineEnothe
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Re: first signs of growth? PICS [Re: TrippyJuice]
    #10069298 - 03/29/09 07:29 PM (15 years, 9 months ago)

id recommend the corn syrup LC tek. its easy

also take a few lids, drill a nice big hole and cover it with lots of silicone(from an auto store). let dry and you have some very nice self-sealing lids. sterilize the needle and pierce right in to innoc / pull out culture.


lc is the way to go, i see growth within 2 days of innoc


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Edited by Enothe (03/29/09 07:30 PM)

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OfflineNineInchNails
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Re: first signs of growth? PICS [Re: Enothe]
    #10069595 - 03/29/09 08:06 PM (15 years, 9 months ago)

I have tried inoculating several LCs using spore syringes and NONE of them panned out at all. My advice would be to save your spores for inoculating jars.

Once you get a fully colonized jar you can rob a hunk of mycelium and plop it into some LCs. That is a sure way to success.

If you’re feelin froggy then by all means squirt some jars of LC, but I have not had any luck what so ever. Once you have some LCs going you could always inoculate more LCs using the LC. Doesn’t hurt to have a ton of that shit.

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OfflineTrippyJuice
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Re: first signs of growth? PICS [Re: NineInchNails]
    #10097437 - 04/02/09 09:22 PM (15 years, 9 months ago)

So.... I inoculated my jars 7 days ago. Unfortunately I'm not seeing any growth in myc. I have them in a shoe box at about 79F, 4 inoculation points, I Followed PF tek exactly as stated!

Today I took my micro pore tape off inoculation holes to hopefully help with colonization.

No smell, no obvious contamination, just some very small white spots hopefully this will grow in the next couple of days.

Any tips would be great fully appreciated!

I'm using a Cambodian spore syringe in BRF/Verm Jars....


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We trippy mane!

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OfflineNineInchNails
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Re: first signs of growth? PICS [Re: TrippyJuice]
    #10097456 - 04/02/09 09:26 PM (15 years, 9 months ago)

COVER those holes. Micropore tape is fine to leave on there.

Statistically ... there are 600,000 particulates in 1 cubic ft of air in your home.

My Cambo grows like wild fire and I have 2 layers of Tyvek covering my jars with one 1/4" hole in the lid. I use 1 RTV high temp silicone inoculation point in the top layer of Tyvek.

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Offlineinit
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Re: first signs of growth? PICS [Re: TrippyJuice]
    #10097505 - 04/02/09 09:32 PM (15 years, 9 months ago)

Give it another week before you start to really worry. Some of my inoculation points needed 2-3 weeks to germinate, so they really take their sweet time sometimes. It's all in the randomness of multispore..

If you have small white patches near the inoculation points, it's probably mycelium. Keep an eye on these, they grow pretty slowly at first but they'll speed up soon enough. Good luck my friend, hope everything turns out well!


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OfflineNineInchNails
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Re: first signs of growth? PICS [Re: NineInchNails]
    #10097509 - 04/02/09 09:32 PM (15 years, 9 months ago)

I saw signs of life in 4 days and I feel lucky. I've heard of people seeing signs WEEKS later than that.

Here's what my jars looked like when I saw signs:


If you leave them alone and covered they will be better off.

Here's what mine looked like 4 days later:


At 10 days:


Just keep the jars sterile and you should be fine.

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OfflineTrippyJuice
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Re: first signs of growth? PICS [Re: NineInchNails]
    #10097653 - 04/02/09 09:48 PM (15 years, 9 months ago)

stop gloating lol! no for real good job and nice close up's what kind of cam do you have those are really nice close ups!


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We trippy mane!

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OfflineNineInchNails
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Re: first signs of growth? PICS [Re: TrippyJuice]
    #10097864 - 04/02/09 10:18 PM (15 years, 9 months ago)

I swear ... I'm not gloating. I failed miserably with my first syringe.

Just a Sony Cybershot DSC-W55. The close pics are using the 'macro' setting.

I was SO PISSED that I actually emailed the seller (of my syringes). Then all of a sudden LIFE occured! ! !

Cambo rips once it starts.

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OfflineTrippyJuice
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Re: first signs of growth? PICS [Re: NineInchNails]
    #10097986 - 04/02/09 10:35 PM (15 years, 9 months ago)

Thanx man! Why did you email the seller?

I'm hoping it starts soon I'm having my doubts!

P.S. I was just joking bout the gloating btw!

I could see spores in my syringe about 15 total that were visible!


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OfflineNineInchNails
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Re: first signs of growth? PICS [Re: TrippyJuice]
    #10100152 - 04/03/09 12:25 PM (15 years, 9 months ago)

Oh ... I inoculated 9 quarts of WBS using a B+ syringe. 20 some days later still no life. In the mean time I purchased 2 more syringes. I inoculated more jars, 5 day later no life. I then emailed the seller of the syringes and next thing I knew I had life in all the jars accept the B+ jars.

Just last night I checked on the B+ jars and there's finally some life in 3 out of 9 ... it took 27 days though. I do fear that some of them may be contaminated, but I'm no expert and those were of my very first batch of jars.

All the other jars are growing nicely.

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