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anakin3197
JEDI MASTER


Registered: 08/21/08
Posts: 1,425
Last seen: 9 months, 2 days
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10067453 - 03/29/09 03:27 PM (15 years, 9 months ago) |
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looks like you reposted the grocery store agar tek, which could easily be found using the search function.
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: anakin3197]
#10067498 - 03/29/09 03:34 PM (15 years, 9 months ago) |
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Well shit!
I guess it is eerily similar.
I guess the main differences are that you have to grind your own agar in the other one.
I posted this thread here http://www.shroomery.org/forums/showflat.php/Number/9900556 a while ago, but something about the grocery store tek didn't satisfy me. I think it's because you have to grind your own agar.
Shit though, that is pretty damn similar.
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EZ Potato-Honey Agar Tek
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Ganja420Boy
Heroic Doser



Registered: 11/15/06
Posts: 1,207
Loc: CoSmoS
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10067531 - 03/29/09 03:38 PM (15 years, 9 months ago) |
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Quote:
mushroomhunter10 said: Are you near any major cities??
Where you at?
Im about 40-50 miles north of Nashville but I have no transportation.
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: Ganja420Boy]
#10067579 - 03/29/09 03:45 PM (15 years, 9 months ago) |
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Next time you're in Nashville try to find some.
Make sure you spend at least ten bucks so you'll have plenty. For me it's $1.25 a packet for 25 grams.
So ten would give you a lot.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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komsija
Stranger

Registered: 03/31/09
Posts: 43
Last seen: 15 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10080571 - 03/31/09 02:02 PM (15 years, 9 months ago) |
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i was making agar plate with a potato recipe...PDA i was doing just the same as was writing here on forums...i clone a oyster mushroom....but i get just a litle colonization after 17 days:(. I dont now what i am doing wrong:(. after 17 days i see colonization , but its not complitly pure white color. Is thet normal?What i am doing wrong?:(
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: komsija]
#10080655 - 03/31/09 02:14 PM (15 years, 9 months ago) |
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If you take some pictures it might help.
It could be a number of things.
Nutrients Temperature Genetics
To name a few.
You should try more than one plate too. Try 5-10 plates.
And yes, it should be nice and white.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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komsija
Stranger

Registered: 03/31/09
Posts: 43
Last seen: 15 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10080989 - 03/31/09 03:04 PM (15 years, 9 months ago) |
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yes i have alredy made a 30 petri dishies, and i shoud take a picture you to see it afcourse....but what temperature is optimal for agar plates after cloning a mushroom, and how long it should take a full colonization for oyster mushroom on PDA agar medium?
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Blutjager
Inhuman


Registered: 06/11/06
Posts: 9,220
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: komsija]
#10081021 - 03/31/09 03:08 PM (15 years, 9 months ago) |
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Book marked 5 for you
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thefinalhours
Student



Registered: 12/15/07
Posts: 252
Last seen: 14 years, 5 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: komsija]
#10081022 - 03/31/09 03:08 PM (15 years, 9 months ago) |
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This is just like the grocery agar tek except that powdered agar is used. Shroominit wrote it and helped me use the powdered agar you have there. Works like a charm.
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: komsija]
#10081027 - 03/31/09 03:08 PM (15 years, 9 months ago) |
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I'm not sure about the time. However, the temperature for most, if not all mycelium is 70-80F.
Try posting this in the cultivation forum.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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komsija
Stranger

Registered: 03/31/09
Posts: 43
Last seen: 15 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10081082 - 03/31/09 03:16 PM (15 years, 9 months ago) |
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ok.thx:)
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: komsija]
#10081090 - 03/31/09 03:18 PM (15 years, 9 months ago) |
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NP
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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