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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique!
#10063153 - 03/28/09 10:59 PM (15 years, 9 months ago) |
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So I recently wanted to try some agar work with some edibles but didn't have a scale to weigh anything. A friend loaned me their scale, I recorded some measurements by using it, then created this PHA-by-Volume technique with great success. I'll never need an expensive digital scale again.
Potato Honey Agar, by volume. You'll need the following items:
 Measuring Cup (Preferably Pyrex) 500ml or more.
 Set of Measuring Spoons - Mine have 5ml engraved on the teaspoon (TSP), and 15ml engraved on the tablespoon (TBS).
 Agar-Agar Powder (Available at Asian food markets).
 Instant Potato Flakes.
 Clear Honey (The Honey Bear Is Fine).
1. Boil some water and fill your measuring cup to 500ml while it's hot. 2. Add 1.5 tablespoons of potato flakes to your measuring cup. This equals approximately 5 grams of potato flakes. 3. Add 1.5 tablespoons of agar powder to your measuring cup. This equals approximately 10 grams of agar powder. 4. Add 2 teaspoons of honey to your measuring cup. This equals approximately 10ml. 5. Stir the mixture well by using your last teaspoon of honey. This gets all of the honey into solution, and helps to stir everything well. 6. Cover your cup with foil and pressure cook it for 30 minutes @ 15 PSI. 7. Give it a little stir before you allow it to cool for a while (BE STERILE!). 8. Pour into your containers of choice before it solidifies.
You don't need petri dishes either. You can use glass cups as well.
Use a glove box or flow hood when opening your pressure cooker and/or stirring your mixture.
If you want to do 1,000ml then simply double everything.
That's it!
It's surprisingly easy, effective, and works great in a pinch!
I hope I could help somebody culture something!
Good Luck
M.H.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
Edited by mushroomhunter10 (03/28/09 11:08 PM)
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10063212 - 03/28/09 11:09 PM (15 years, 9 months ago) |
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excellent.. the only thing that I see, is just the fact that maybe those exact brands will not be available everywhere, and will have different thicknesses and different densities with the flakes and honey, respectively..
It should work, as the weight should only vary slightly, but it is something to consider..
either way, what pothead that grows mushies doesnt have a digi? lol.. gotta have some way to know what your new bag weighs... lol
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: DJYoshaBYD]
#10063254 - 03/28/09 11:16 PM (15 years, 9 months ago) |
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I know what you mean but I don't have a digital lol!!
All those years of poking smot and I don't have a digi! Believe that s**t? 
I had twenty dishes of edibles going, every single one colonized. Just FYI.
Thanks man. I'm just hoping to help out the poor folks like myself lol!
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10063272 - 03/28/09 11:19 PM (15 years, 9 months ago) |
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yeah.. for sure... check out ebay.. they have killer deals on digi's... glad to hear it goes well..
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: DJYoshaBYD]
#10063278 - 03/28/09 11:20 PM (15 years, 9 months ago) |
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Thanks man.
Good luck to ya!
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10063322 - 03/28/09 11:30 PM (15 years, 9 months ago) |
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no problem.. what type of edibles are you growing? how are you going about cooking them? im a home-style chef, of sorts.. lol
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ScavengerType



Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10063336 - 03/28/09 11:32 PM (15 years, 9 months ago) |
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you could include the amount of dextrose to add instead of honey if using dextrose. It's a nice tek though you should put it in your journal so I can quick reference it easy if I ever need to.
personally I like to make my PDA from real potatoes because it's both a project and a meal, but flakes are good too.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
Edited by ScavengerType (03/28/09 11:34 PM)
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: DJYoshaBYD]
#10063342 - 03/28/09 11:34 PM (15 years, 9 months ago) |
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Haven't fruited yet.
Some Oysters, and King Oysters.
ostreatus and eryngii
PM me if you want to chat about them. Got any good recipes? Send 'em my way.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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DJYoshaBYD


Registered: 04/07/07
Posts: 2,405
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10063350 - 03/28/09 11:35 PM (15 years, 9 months ago) |
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excellent.. sorry for the minor thread jack.. lol.. im lit and poor and hungry.. haha
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: ScavengerType]
#10063362 - 03/28/09 11:38 PM (15 years, 9 months ago) |
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Quote:
ScavengerType said: you could include the amount of dextrose to add instead of honey if using dextrose. It's a nice tek though you should put it in your journal so I can quick reference it easy if I ever need to.
personally I like to make my PDA from real potatoes because it's both a project and a meal, but flakes are good too.
Not sure how much dextrose to use. I would think 5 grams maybe? You can add to this tek if you want to and I can update it.
Grab your scale, throw on the right amount of dextrose for a 500ml solution and tell me what it was in teaspoon(s), or tablespoon.
Thanks man. PM me your results if you ever try it.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10063365 - 03/28/09 11:38 PM (15 years, 9 months ago) |
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No no... I wasn't implying.
I'd like to discuss recipes if you have any.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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ScavengerType



Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10063864 - 03/29/09 01:34 AM (15 years, 9 months ago) |
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I got ~ 1.2 tbsp for both agar and dextrose.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: ScavengerType]
#10064974 - 03/29/09 09:22 AM (15 years, 9 months ago) |
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Are there any milliliters stamped on the spoons?
Thanks.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10065091 - 03/29/09 09:54 AM (15 years, 9 months ago) |
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nope nada sorry I could do 10 scoops of water into a measuring cup to get an accurate approximation.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: ScavengerType]
#10066857 - 03/29/09 02:08 PM (15 years, 9 months ago) |
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Yeah that would be cool.
I'm thinking yours might 1ml larger. 
I think either way it would work though, even 1.2 or 1.5 is barely a difference. Thanks for that though.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10067157 - 03/29/09 02:45 PM (15 years, 9 months ago) |
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Just FYI for everybody.
I have seen recipes that call for 7-12 grams of dextrose, so there is a large window to play with.
If anybody has trouble or no results with this then please let me know ASAP and I'll modify it. It has worked for myself, and another friend superbly though.
Happy culturing!
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
|
ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10067258 - 03/29/09 02:57 PM (15 years, 9 months ago) |
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haha yes if anything the amount of agar would affect the situation but I doubt so in those small of quantities.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: ScavengerType]
#10067309 - 03/29/09 03:06 PM (15 years, 9 months ago) |
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Yeah I've even seen variable amounts of agar too. A smaller window, but probably won't make much of a difference.
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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Ganja420Boy
Heroic Doser



Registered: 11/15/06
Posts: 1,207
Loc: CoSmoS
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: mushroomhunter10]
#10067374 - 03/29/09 03:15 PM (15 years, 9 months ago) |
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I want to try this but I cant find agar around here or a asian food market and ordering online isnt a option
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mushroomhunter10
Jack-Of-All-Trades



Registered: 10/04/08
Posts: 3,360
Loc: Midwest
Last seen: 9 years, 7 months
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Re: Want to work with agar but don't have a digital scale? Try this PHA-by-Volume technique! [Re: Ganja420Boy]
#10067433 - 03/29/09 03:24 PM (15 years, 9 months ago) |
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Are you near any major cities??
Where you at?
-------------------- Imagine if you needed it and it wasn't there... GIVE BLOOD
Get a free (PAINLESS) bone marrow testing kit and help save lives HERE.
Jesus if you're reading this, please come back already. We need you now more than ever!
The U.S. Constitution!
Best WBS Tek
EZ Potato-Honey Agar Tek
MY TRADES
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