|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
|
.
#10061030 - 03/28/09 05:08 PM (15 years, 9 months ago) |
|
|
.
Edited by Peterthinks (04/02/09 06:40 PM)
|
Doctor
Strangled




Registered: 03/05/05
Posts: 155
Last seen: 10 years, 6 months
|
Re: easy easy plunger print syringe Tek [Re: Peterthinks]
#10061174 - 03/28/09 05:43 PM (15 years, 9 months ago) |
|
|
I like the look of that, and well worth a try.  Ty
|
scifipirate
Jug Fuckler



Registered: 01/17/09
Posts: 870
Loc: FL
Last seen: 2 months, 28 days
|
Re: easy easy plunger print syringe Tek [Re: Doctor]
#10061185 - 03/28/09 05:46 PM (15 years, 9 months ago) |
|
|
That seems just crazy enough to work!
|
Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
|
|
.
Edited by Peterthinks (04/02/09 06:48 PM)
|
Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
|
|
.
Edited by Peterthinks (04/02/09 06:49 PM)
|
azshroomer
no1

Registered: 01/07/09
Posts: 282
|
Re: easy easy plunger print syringe Tek [Re: Peterthinks]
#10061346 - 03/28/09 06:13 PM (15 years, 9 months ago) |
|
|
Yeah, but wont the syringe become unsterile while the plunger is removed, and then the plunger will definitly not be sterile while allowing spores to fall on it...
Correct?
|
MycoAu
5thKingdomCome


Registered: 07/18/07
Posts: 1,047
Last seen: 4 years, 30 days
|
Re: easy easy plunger print syringe Tek [Re: azshroomer]
#10061361 - 03/28/09 06:15 PM (15 years, 9 months ago) |
|
|
Not if you open everything in front of a hood, then close everything back up to sit long enough for printing. Then, open again in front of the hood to hydrate the spores. Cap, label and store. It's a good idea in my book.
You might just try printing (sterile- or as close as it ever gets), on foil, opening the syringe in a sterile field and dropping a small piece of the print into the syringe, the add water, shake, cap label, etc. (Technique is called backloading a syringe, btw.)
Edited by MycoAu (03/28/09 06:18 PM)
|
Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
|
|
.
Edited by Peterthinks (04/02/09 06:49 PM)
|
ScavengerType



Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
|
Re: easy easy plunger print syringe Tek [Re: Peterthinks]
#10063393 - 03/28/09 11:47 PM (15 years, 9 months ago) |
|
|
Roomie Fucked My Butt ***SERIOUS REPLIES ONLY***
Not kidding first thread to come up when I searched "backloading"
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
|
Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
|
|
.
Edited by Peterthinks (04/02/09 06:51 PM)
|
Shroominit
Part Time Mycologist



Registered: 11/29/08
Posts: 4,662
|
Re: easy easy plunger print syringe Tek [Re: Peterthinks]
#10065316 - 03/29/09 10:38 AM (15 years, 9 months ago) |
|
|
I am with them on the sterility problem. You can't wait for spores to drop straight down in front of a flowhood, it just won't happen (not to mention running the hood for hours). If not in front of a flowhood, you still need good FAE in your FC so you may have spores (bad ones) coming in that way.
The only way I see this working is in a monotub-like setup where the holes are filled with polyfil and the lid stays on all the time.
|
MEEZIE
Incognito


Registered: 02/28/09
Posts: 711
Loc: USA
|
Re: easy easy plunger print syringe Tek [Re: Shroominit]
#10065589 - 03/29/09 11:39 AM (15 years, 9 months ago) |
|
|
worth a try
|
FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
|
Re: easy easy plunger print syringe Tek [Re: Shroominit]
#10065709 - 03/29/09 11:58 AM (15 years, 9 months ago) |
|
|
Quote:
Shroominit said: I am with them on the sterility problem. You can't wait for spores to drop straight down in front of a flowhood, it just won't happen (not to mention running the hood for hours). If not in front of a flowhood, you still need good FAE in your FC so you may have spores (bad ones) coming in that way.
The only way I see this working is in a monotub-like setup where the holes are filled with polyfil and the lid stays on all the time.
You don't need to wait for the spores to drop. Just rub the plunger on the gills like a swab.
--------------------
Quick WBS Prep
|
MycoAu
5thKingdomCome


Registered: 07/18/07
Posts: 1,047
Last seen: 4 years, 30 days
|
Re: easy easy plunger print syringe Tek [Re: FooMan]
#10065868 - 03/29/09 12:16 PM (15 years, 9 months ago) |
|
|
For the smart-ass who can't see past all the porn:
http://imaging-radiology-oncology-technologist.advanceweb.com/Editorial/Content/Editorial.aspx?CC=10467
"Keep backloading syringes to keep up with demand. Try to have at least two syringes ready at all times. This process might actually take 10-18 syringes per level."
It's used in the pharmaceutical field all the time. This is a perfect example of why newbs can't ever search something out to find it. Googled "backloading syringe", last link of the first page of results. Here's a link for the same technique used in the dental office setting: http://www.duxdental.com/vd_tr.htm
I'm sure there are hundreds of links available.
|
Shroominit
Part Time Mycologist



Registered: 11/29/08
Posts: 4,662
|
Re: easy easy plunger print syringe Tek [Re: FooMan]
#10066406 - 03/29/09 01:17 PM (15 years, 9 months ago) |
|
|
Quote:
FooMan said: You don't need to wait for the spores to drop. Just rub the plunger on the gills like a swab.
Good point. Don't we generally assume all mushroom fruits to not be sterile because of the FAE that comes with fruiting conditions? Isn't this why we use swabs on agar, rather than going straight to grain or putting the swabs in water and using it as a syringe?
|
ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
|
Re: easy easy plunger print syringe Tek [Re: Shroominit]
#10066433 - 03/29/09 01:19 PM (15 years, 9 months ago) |
|
|
swabs... good idea.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
|
FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
|
Re: easy easy plunger print syringe Tek [Re: Shroominit]
#10067987 - 03/29/09 04:42 PM (15 years, 9 months ago) |
|
|
Quote:
Shroominit said:
Quote:
FooMan said: You don't need to wait for the spores to drop. Just rub the plunger on the gills like a swab.
Good point. Don't we generally assume all mushroom fruits to not be sterile because of the FAE that comes with fruiting conditions? Isn't this why we use swabs on agar, rather than going straight to grain or putting the swabs in water and using it as a syringe?
If that were the case syringes wouldn't even be sold.
--------------------
Quick WBS Prep
|
Shroominit
Part Time Mycologist



Registered: 11/29/08
Posts: 4,662
|
Re: easy easy plunger print syringe Tek [Re: FooMan]
#10068047 - 03/29/09 04:49 PM (15 years, 9 months ago) |
|
|
Quote:
FooMan said: If that were the case syringes wouldn't even be sold.
Hm, seems to me that when a mushroom sporulates, the spores have not been exposed to anything and we use that to our advantage to get clean prints. The clean prints are then used to make syringes.
Unless you mean that vendors somehow use swabs or this backloading method to make syringes?
|
FooMan



Registered: 02/02/05
Posts: 8,957
Loc: Earth
|
Re: easy easy plunger print syringe Tek [Re: Shroominit]
#10068081 - 03/29/09 04:52 PM (15 years, 9 months ago) |
|
|
You just said we assume all fruits aren't sterile. Vendors make their syringes straight from prints as far as I know. Those prints are sterile unless proper precautions weren't taken.
--------------------
Quick WBS Prep
Edited by FooMan (03/29/09 08:51 PM)
|
Shroominit
Part Time Mycologist



Registered: 11/29/08
Posts: 4,662
|
Re: easy easy plunger print syringe Tek [Re: FooMan]
#10068121 - 03/29/09 04:56 PM (15 years, 9 months ago) |
|
|
The fruits aren't, but the spores that are between gills are. When you use a swab and touch the gills, you have a pretty good chance of picking up contams with it, when the spores drop, they have a very good chance of being sterile.
At this point, I'd like to see this method done, if it works consistently without contams, I agree it would be a great method.
|
tripz



Registered: 03/27/05
Posts: 154
Last seen: 7 years, 3 months
|
Re: easy easy plunger print syringe Tek [Re: Peterthinks]
#10068161 - 03/29/09 05:01 PM (15 years, 9 months ago) |
|
|
I like this idea. Nice
--------------------
irc.danknug.com
#shroomery / #cultivation
|
JaComet
Old Hand

Registered: 11/12/02
Posts: 347
Loc: Out Yonder
|
Re: easy easy plunger print syringe Tek [Re: tripz]
#10078748 - 03/31/09 06:36 AM (15 years, 9 months ago) |
|
|
Excellent.
--------------------
|
HorusTh3Chrous


Registered: 11/14/05
Posts: 955
|
Re: easy easy plunger print syringe Tek [Re: azshroomer]
#10079153 - 03/31/09 09:10 AM (15 years, 9 months ago) |
|
|
Quote:
azshroomer said: Yeah, but wont the syringe become unsterile while the plunger is removed, and then the plunger will definitly not be sterile while allowing spores to fall on it...
Correct?
this is what i was thinking.. i like the jar method with self sealing injection sites.
|
navyseals101
Stranger



Registered: 09/16/04
Posts: 1,053
Last seen: 7 years, 6 months
|
Re: easy easy plunger print syringe Tek [Re: HorusTh3Chrous]
#10082714 - 03/31/09 06:58 PM (15 years, 9 months ago) |
|
|
I dont get it, how do you add the plunger back into the barrel without getting contamns in?
-------------------- "Ten thousand flowers in spring
the moon in autumn,
a cool breeze in summer,
snow in winter.
If your mind isn't clouded by unnecessary things,
this is the best season of your life."
-wumen
|
Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
|
|
.
Edited by Peterthinks (04/02/09 06:46 PM)
|
ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
|
Re: easy easy plunger print syringe Tek [Re: Peterthinks]
#10090114 - 04/01/09 08:38 PM (15 years, 9 months ago) |
|
|
nice, now that I'd do, but what's with the perlite? It doesn't seem necessary for anything outside of holding the plungers up.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
|
Peterthinks
(Caulking) gun for hire

Registered: 11/10/04
Posts: 2,379
|
|
.
Edited by Peterthinks (04/02/09 06:47 PM)
|
ScavengerType


Registered: 01/24/08
Posts: 5,784
Loc: The North
Last seen: 11 years, 3 months
|
Re: easy easy plunger print syringe Tek [Re: Peterthinks]
#10090263 - 04/01/09 09:00 PM (15 years, 9 months ago) |
|
|
awesome, nice job.
-------------------- "Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club
|
LokelYokel
I Gro Fuud


Registered: 08/24/08
Posts: 382
Loc: Deep in the Heart
|
Re: easy easy plunger print syringe Tek #UPDATED YOU PICKY BASTARDS!# [Re: Peterthinks]
#10090799 - 04/01/09 10:25 PM (15 years, 9 months ago) |
|
|
Quote:
It's a bit of a tight fit but it works, yeah you're so tight baby! Just wiggle a bit, yeah that's it, take it all!!! My big black plunger into your tight body! Don't worry honey, I'm totally sterile!
Ahhhhhh!!!!
What the hell is wrong with me?
If I can stop laughing I'll read the rest of the edit!
-------------------- ReUse! RePurpose! ReCycle! "Mom says use it again!"
|
|