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lgm0420
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Registered: 02/18/09
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Never been in this situation before...
#10057635 - 03/27/09 11:57 PM (15 years, 9 months ago) |
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Alright, so first ill start off with the background. I'm doing one of my first bulk attempts and i messed up the ratio of spawn to substrate. Because of that, my recolonization was extremely slow, actually at this point i would say its stalled out at about 25%. I left it sitting for a few days and when i came back it was hit with cobweb, or what I'm pretty sure is cobweb. I started treating it with h202 at a 1:3 ratio of 3% h2o2 to water. Is this too strong? Either way, my main concern is what i should do here. Should i just birth the bulk and set it up for fruiting? Is it even worth it with the amount its colonized? Could i get fruits? Is the cobweb going to prevent me from getting anything outta this? Should i bother casing it since its already contamed? I have obviously put some work and time into this, so I'm would like to get anything i can outta it. I've never been in this situation before and could really use some advice from anyone who might have dealt with something like this before.
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potatonet
Quantum Scientist



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Re: Never been in this situation before... [Re: lgm0420]
#10057680 - 03/28/09 12:10 AM (15 years, 9 months ago) |
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honestly if it hasnt colonized and you have contam, dump it.
If it was colonized and about to pin then you would have a chance.
uphill battle if you ask me, spend the time re-doing it. you can still try and save it but I wouldnt, the spores will spread.
-------------------- In the Quechua language of Peru there are over 1000 words for "potato"
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Typhus
ALGUAALGUALGUALGUALGUALGUAL




Registered: 09/30/08
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Loc: Minnesnota
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Re: Never been in this situation before... [Re: potatonet]
#10057699 - 03/28/09 12:15 AM (15 years, 9 months ago) |
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If you treated with 1% h2o2 the cobweb should have almost instantly shriveled up and died, if it didn't do anything it might just be mycelium or another contam, good pics help in this department. IDK if throwing it out is a good idea yet, might just wait and see, but the moment it turns green or teal color I would.
Also if its to wet/not wet enough it wont colonize, until you dry or moisten it.
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ARE YOU JENNA JAMESON BECAUSE I AM SERIOUSLY STIFF
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lgm0420
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Re: Never been in this situation before... [Re: Typhus]
#10057722 - 03/28/09 12:21 AM (15 years, 9 months ago) |
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Well i sprayed it with the h2o2 earlier today, probably a few hours ago... its a grey color, no green or teal yet. If it was that color i wouldnt even bother... i just would hate to throw it, its taken a while to get to this point. Should i work it with the h2o2 for a few days and go from there? Is there a chance for it to pin where it colonized? should i case it if the cobweb dissapears?
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Typhus
ALGUAALGUALGUALGUALGUALGUAL




Registered: 09/30/08
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Re: Never been in this situation before... [Re: lgm0420]
#10057828 - 03/28/09 12:44 AM (15 years, 9 months ago) |
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if the cobweb disappears and it colonization, it'd be perfect. all i'm saying is letting it sit for a few days/weeks covered wont hurt much so long as you limit the amount of spores you drag around with you after lookin at it. If it doesnt colonize and the contam persists, as potonet said, it is probably best to cut your loses.
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ARE YOU JENNA JAMESON BECAUSE I AM SERIOUSLY STIFF
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The shroomy 1
Luminous beings surround me




Registered: 03/27/07
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Re: Never been in this situation before... [Re: lgm0420]
#10057855 - 03/28/09 12:52 AM (15 years, 9 months ago) |
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Quote:
lgm0420 said: Alright, so first ill start off with the background. I'm doing one of my first bulk attempts and i messed up the ratio of spawn to substrate. Because of that, my recolonization was extremely slow, actually at this point i would say its stalled out at about 25%. I left it sitting for a few days and when i came back it was hit with cobweb, or what I'm pretty sure is cobweb. I started treating it with h202 at a 1:3 ratio of 3% h2o2 to water. Is this too strong? Either way, my main concern is what i should do here. Should i just birth the bulk and set it up for fruiting? Is it even worth it with the amount its colonized? Could i get fruits? Is the cobweb going to prevent me from getting anything outta this? Should i bother casing it since its already contamed? I have obviously put some work and time into this, so I'm would like to get anything i can outta it. I've never been in this situation before and could really use some advice from anyone who might have dealt with something like this before.
Many unanswered questions here. ESPECIALLY if this is your first bulk attempt. What was your bulk substrate? What were the procedures you took to "prepare" the bulk substrate? What was the spawn ratio? How did you know that it was 25% colonized? I've never been able to see inside my bulk substrate! If you have a contam........ Sorry. Dispose and start over. This is your FIRST attempt at "The next level". Work out the "Bugs" and start fresh.
--------------------
AMU Q&A thread.
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lgm0420
Stranger
Registered: 02/18/09
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Last seen: 11 years, 7 months
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Re: Never been in this situation before... [Re: The shroomy 1]
#10057884 - 03/28/09 12:59 AM (15 years, 9 months ago) |
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Used a horse poo/straw/coir/verm mix... 25% is a guess, the sides of the tub arent covered up yet, so just whats breaking the surface and what i can see from the sides its an estimate. I bought the poo/straw already pastuerized. Let the coir/verm sit in a bucket with water originally at 180 F sit over night... had some gypsum mixed in also. I used two quart jars for a 3 and a half inch thick substrate in a 64 quart rubbermaid container.
Its seeming like dumping it is the concensus, just hard to do it, alot of time, research and heart put into it, you know?
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HALFemptyJOSH
Magician



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Re: Never been in this situation before... [Re: lgm0420]
#10058029 - 03/28/09 01:34 AM (15 years, 9 months ago) |
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have a picture?? their are procedures where you can cut the mold away from what you have but instantly, i would say dunk it in dark for a day and keep in dark with a new small "glad ware/slightly bigger" casing for another 3-5 days and reintroduce it to light, airflow, and a 10 degree decrease in teperature... you may have to re-pasteurize the casing mix but that all depends...
did you try one monotub??
you shouldn't go to the fruiting stage til' around 80%-90% mycelium covers the casing. sometimes people will put an extra thin layer of moist vermiculite over it. litterally two seconds before the fruiting stage. who knows i don't have a degree
Edited by HALFemptyJOSH (03/28/09 01:52 AM)
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veda_sticks
Cultivator




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Re: Never been in this situation before... [Re: HALFemptyJOSH]
#10058444 - 03/28/09 04:43 AM (15 years, 9 months ago) |
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Quote:
HALFemptyJOSH said: have a picture?? their are procedures where you can cut the mold away from what you have but instantly, i would say dunk it in dark for a day and keep in dark with a new small "glad ware/slightly bigger" casing for another 3-5 days and reintroduce it to light, airflow, and a 10 degree decrease in teperature... you may have to re-pasteurize the casing mix but that all depends...
did you try one monotub??
you shouldn't go to the fruiting stage til' around 80%-90% mycelium covers the casing. sometimes people will put an extra thin layer of moist vermiculite over it. litterally two seconds before the fruiting stage. who knows i don't have a degree
Bad advice IMO.
Cutting out contamination is a bad move, which just spreads it further. Fruiting before full colinisaiton is also a bad idea, if you introduce your tray to fruiting conditions the uncolinised portions will likely contaminate.
Are absoltly sure you have cobweb, so many new cultivators mistake cobweb for thin whispy growth that hasnt thickened up yet. Also note that treating cobweb should be done with 3% h202, not diluted. Spray the effected area and it should fizzle away, spray again after 12 hours.
You really need to post a picture before anyone can really tell for sure.
Also, you got your pre-pasturised manure from a trusted vendor?
--------------------
PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
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MycoMario
:D



Registered: 03/26/09
Posts: 134
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Re: Never been in this situation before... [Re: HALFemptyJOSH]
#10058772 - 03/28/09 07:39 AM (15 years, 9 months ago) |
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Quote:
HALFemptyJOSH said: i would say dunk it in dark for a day and keep in dark with a new small "glad ware/slightly bigger" casing for another 3-5 days and reintroduce it to light, airflow, and a 10 degree decrease in teperature...
how on earth would he dunk that? i am not following your logic.
also: cubes don't need a decrease in temperature.
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shroomanxiety
Stranger with candy



Registered: 01/31/09
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Re: Never been in this situation before... [Re: MycoMario]
#10059206 - 03/28/09 09:57 AM (15 years, 9 months ago) |
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Quote:
MycoMario said:
Quote:
HALFemptyJOSH said: i would say dunk it in dark for a day and keep in dark with a new small "glad ware/slightly bigger" casing for another 3-5 days and reintroduce it to light, airflow, and a 10 degree decrease in teperature...
how on earth would he dunk that? i am not following your logic.
also: cubes don't need a decrease in temperature.
i think the temp decrease was suggested not for cold shock but because contams grow way faster with warmth
-------------------- Drunk posting expert! don't try at home! or do, and you can look like an A**hole too.
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george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,777
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Re: Never been in this situation before... [Re: lgm0420]
#10059247 - 03/28/09 10:07 AM (15 years, 9 months ago) |
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Toss it. Rarely(if ever)will you get any benefit from keeping a contaminated casing.
-------------------- KRAMER CAKES


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HALFemptyJOSH
Magician



Registered: 09/01/03
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george is right and last night i was a little drunk... umm i guess i can't exactly explain what i mean but it's the only way to salvage a contammed casing... either you know what i'm talking about and it might work or you don't and fuck it just toss it.. more than enough people have said that to convince you
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MycoMario
:D



Registered: 03/26/09
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Re: Never been in this situation before... [Re: HALFemptyJOSH]
#10059529 - 03/28/09 11:17 AM (15 years, 9 months ago) |
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i still don't see how anyone could dunk substrate that hadn't colonized.
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george castanza
Lord Of The Idiots!


Registered: 10/21/02
Posts: 8,777
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Re: Never been in this situation before... [Re: MycoMario]
#10059618 - 03/28/09 11:40 AM (15 years, 9 months ago) |
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Quote:
MycoMario said: i still don't see how anyone could dunk substrate that hadn't colonized.
I agree, this is bad advice
-------------------- KRAMER CAKES


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lgm0420
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Registered: 02/18/09
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Last seen: 11 years, 7 months
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Alright, well hopefully some of you guys will be back around to continue with help cuase i have an update. I sprayed wiht a 1:3 ratio of h2o2 to water and the cobweb is almost all gone after about 12 hours or so. I think i can get rid of it, but im still left with the stalled myc growth. What can i do about this? My ratio is way off, i used about half the spawn i probably needed for proper colonization. Is it possible itll never fully colonize? Its definetly stopped growing for hte last week, what can i do?
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lgm0420
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Last seen: 11 years, 7 months
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Re: Never been in this situation before... [Re: lgm0420]
#10060363 - 03/28/09 02:51 PM (15 years, 9 months ago) |
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My substrate has been colonizating for over two weeks now, could my moisture content be the problem after all this time? Is there a way to fix that? Also i havent cased it yet, its just my substrate thats colonizing.
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lgm0420
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Re: Never been in this situation before... [Re: lgm0420]
#10061700 - 03/28/09 07:05 PM (15 years, 9 months ago) |
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anyone?
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Typhus
ALGUAALGUALGUALGUALGUALGUAL




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Re: Never been in this situation before... [Re: lgm0420]
#10067449 - 03/29/09 03:26 PM (15 years, 9 months ago) |
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IF you have standing water in the bottom/it is sorta muddish, yes it is to wet IF it is to dry, hmm, idk how to describe that.
either way, its probley time to start next tub anyway that said horse poo/straw/coir/verm is a wierd mix to try first go. Next time you might want to try like 4 parts hpoo/1part verm. http://www.shroomery.org/8397/Horse-poo-substrate-preparation
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ARE YOU JENNA JAMESON BECAUSE I AM SERIOUSLY STIFF
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lgm0420
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Re: Never been in this situation before... [Re: Typhus]
#10068641 - 03/29/09 06:18 PM (15 years, 9 months ago) |
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The problem isnt too wet, and its not too dry, the problem is stalled myc growth. Im saying its possible that the moisture content is off from sitting for so long for colonization. Wondering if theres anything i can do to fix that problem if that were it, and wondering what else could explain the stall in myc growth. I just got rid of my cob web problem i dont see the point in just tossing this tub after a big victory over contams! can anyone help me in getting my myc to fully colonize?!!?
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