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InvisibleFloydian
veteran
Registered: 05/13/00
Posts: 1,022
wots uhhh the deal
    #1005202 - 10/30/02 02:49 AM (21 years, 5 months ago)

Hi guys. I've done some searching and read the faqs but I just wanted to be perfectly clear on something before i do anything. I know you guys gotta hate newbie questions.

Innoculated jars 4 days ago and no signs of growth. There is a little condensation on the inside of jars (a few drops here and there) and have kinda gotten the impression that the holes used for innoculation shouldn't be covered with tape. They are currently covered with scotch tape.

Should i remove the tape? I found a few posts pertaining to this and they said its best to remove tape and flip jars. But I wasn't sure what stage of colinization they were refering to.

So should I flip jars and/or remove tape at this point to get colinization going?

Thanks in advance.


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Don't squeeze the pancake batter

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OfflineSmashedMe
Stranger
Registered: 03/10/02
Posts: 414
Loc: Antarctica
Last seen: 18 years, 2 months
Re: wots uhhh the deal [Re: Floydian]
    #1005217 - 10/30/02 03:04 AM (21 years, 5 months ago)

Give them more time. I never used tape on my innoculation holes. But let them sit longer it takes time. Also the area you are incubating in may play a nice role on this.

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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 1 month, 19 days
Re: wots uhhh the deal [Re: Floydian]
    #1005255 - 10/30/02 04:38 AM (21 years, 5 months ago)

It can sometimes take a good two weeks before you see growth. Give them time... (I have read that it can take up to a month in some cases, but I have never seen it take that long myslef)


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Just another spore in the wind.

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OfflineViveka
refutation bias
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Registered: 10/21/02
Posts: 4,061
Last seen: 7 years, 5 months
Re: wots uhhh the deal [Re: Floydian]
    #1005505 - 10/30/02 08:36 AM (21 years, 5 months ago)

I've left my jars taped at least until i get some solid colonization in the upper portion of the jar to help ward off contams. But if you untape em now it might help speed colonization.

I wouldn't flip the jars till they are about 60% or 70% colonized, otherwise it defeats the purpose of flipping. (This is also when I untape the holes.)

Do whatever seems right to you.

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OfflineTheta
TranscendentalExplorer

Registered: 10/29/02
Posts: 352
Last seen: 18 years, 3 months
Re: wots uhhh the deal [Re: Viveka]
    #1005741 - 10/30/02 10:07 AM (21 years, 5 months ago)

In reply to:

I wouldn't flip the jars till they are about 60% or 70% colonized, otherwise it defeats the purpose of flipping. (This is also when I untape the holes.)



In this particular scenario, what IS the purpose of flipping the jars?

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OfflinePenguin
You can't be me I'm a Rockstar

Registered: 02/10/02
Posts: 1,830
Loc: On the Arctic ice shelf.
Last seen: 1 year, 1 month
Re: wots uhhh the deal [Re: Theta]
    #1005751 - 10/30/02 10:10 AM (21 years, 5 months ago)

Patience young grasshopper.


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The fear of loss is always greater than the desire for gain

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InvisibleFloydian
veteran
Registered: 05/13/00
Posts: 1,022
Re: wots uhhh the deal [Re: Penguin]
    #1007716 - 10/30/02 07:55 PM (21 years, 4 months ago)

Thanks guys. I'll just wait it out and see what happens. No vermiculite layer on top so i'm gonna leave the tape on for now.


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Don't squeeze the pancake batter

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InvisibleFloydian
veteran
Registered: 05/13/00
Posts: 1,022
Re: wots uhhh the deal [Re: Penguin]
    #1026650 - 11/06/02 01:10 AM (21 years, 4 months ago)

So its been about 2 weeks now and still no sign of germination or growth of any kind. What are some options to concider here?


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Don't squeeze the pancake batter

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OfflineStS
treetoker
Male User Gallery

Registered: 07/31/00
Posts: 1,821
Loc: Earth
Last seen: 4 years, 3 months
Re: wots uhhh the deal [Re: Floydian]
    #1026662 - 11/06/02 01:15 AM (21 years, 4 months ago)

I'd say give it at least one more week and if still nothing, then it could be a number of things. Did you let the jars cool long enough before innoculating? What temp are you keeping the jars in? What is you substrate?


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"You took too much, man....too much, too much!"

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Invisiblecarbonhoots
old hand

Registered: 09/11/01
Posts: 1,351
Loc: BC Canada
Re: wots uhhh the deal [Re: Floydian]
    #1026681 - 11/06/02 01:28 AM (21 years, 4 months ago)

To germinate spores, 86F is good for a couple days, then 80-84F fo growth...


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  -I'd rather have a frontal lobotomy than a bottle in front of me

CANADIAN CENTER FOR POLICY ALTERNATIVES

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