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Offline3500lt
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Registered: 01/09/08
Posts: 250
Last seen: 8 years, 7 months
RYE. VS. WBS
    #8245409 - 04/06/08 03:13 AM (15 years, 11 months ago)

well i have experience with both now. and i seem to have alot more contamination with WBS.. i dont know why. i prepare my WBS the same as the Rye berries. soak for 24 hours, strain, rinse, and pc for 1 hour at 15psi. with the Rye my contamination ratio is around 15% when i do 90quart jars. i have done 2 rounds with the WBS and i feel im waisting my time and money with it. Its alot of work to be throwing 75% of my birdseed away, and i am not doing anything different than with the rye. I do grain transfers right now, until my lc`s are ready, and i know i am giving a little room for contams by doing that, but 75% is rediculous. I lysol alot and the jars are not open for more than a couple seconds. i dont understand. im still new and learning like everyone else here, but its frustrating when your waisting that much time preparing all that spawn for nothing.

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Offlineeleven34
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Re: RYE. VS. WBS [Re: 3500lt]
    #8245420 - 04/06/08 03:21 AM (15 years, 11 months ago)

I've read to PC for an 1.5 hours with both rye and wbs.

That could possibly be it.

That's the only thing I can think of. If not that. I would just stick to rye if that's working out for ya.


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"Get your damn hands off me sesame cake"

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Offline3500lt
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Re: RYE. VS. WBS [Re: eleven34]
    #8245429 - 04/06/08 03:25 AM (15 years, 11 months ago)

yea ill try pcing longer, takes 2 days to do all those jars with prep and everything, but time is on my side. trial and error.

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Offline3500lt
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Registered: 01/09/08
Posts: 250
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Re: RYE. VS. WBS [Re: 3500lt]
    #8245439 - 04/06/08 03:32 AM (15 years, 11 months ago)

would constructing a large "flow hood" help my problem?

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Offlineeleven34
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Re: RYE. VS. WBS [Re: 3500lt]
    #8245480 - 04/06/08 03:59 AM (15 years, 11 months ago)

Flow hoods are always good to have. It's the cost that doesn't allow a lot of us to have one.


--------------------
"Get your damn hands off me sesame cake"

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Offlinethedoc8
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Re: RYE. VS. WBS [Re: eleven34]
    #8245594 - 04/06/08 06:49 AM (15 years, 11 months ago)

I always pc for 1.5 and in over a year have never had one rye contaminated jar. I use nothing special just the stove top and be clean.

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OfflineHybridprX
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Re: RYE. VS. WBS [Re: thedoc8]
    #8245761 - 04/06/08 08:12 AM (15 years, 11 months ago)

Grains require a hour a and half at 15psi.

You need to be doing your grain transfers in either a glove box or a flow hood that is why your contamination rate is so high. Stick with G2G's for large grows, you can see contams in a G2G Master jar, you cant in LC if your LC is bunk and you knock up 90 qts you're really going to be pissed then.


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Offline3500lt
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Re: RYE. VS. WBS [Re: HybridprX]
    #8249227 - 04/07/08 12:57 AM (15 years, 11 months ago)

Lol yea that makes sense. im doing a few different clones with the lc,s and they look good so far. had to clone the PE because i cant get any prints off of them. but yea im goona build a nice flow hood. thanks for the advice on pcing. 1.5 hours sounds good to me. cant believe i have done so many jars and not pc'd them long enough

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InvisibleBlutjager
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Re: RYE. VS. WBS [Re: 3500lt]
    #8249618 - 04/07/08 06:55 AM (15 years, 11 months ago)

Yea,and hr and a half and a glovebox or flowhood will help allot,if it makes you feel better though I would like to say that I always have a better success rate with rye over birdseed no matter what,its just a better grain in my opinion

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InvisiblePrisoner#1
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Re: RYE. VS. WBS [Re: HybridprX]
    #8251056 - 04/07/08 02:49 PM (15 years, 11 months ago)

Quote:

HybridprX said:
Grains require a hour a and half at 15psi.





I only PC grains at 15psi for 90 minutes :smirk:

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Offlineweretheshit
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Re: RYE. VS. WBS [Re: Prisoner#1]
    #8251252 - 04/07/08 03:30 PM (15 years, 11 months ago)

different grains have different water retaining capabilities. WBS is usually better than other grains for this because of its variety of grains. Try working with WBS for a while, then switching to straight millet, or straight milo. BIG difference in the way they absorb water. This is likely why, IMO, that you are having success with the rye over the WBS. Your used to the rye's water retainment, and not the WBS. ALL grains will absorb differently. Just a guess though, you likely should PC longer too.

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OfflineActionExpress
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Re: RYE. VS. WBS [Re: weretheshit]
    #10012216 - 03/21/09 05:13 AM (15 years, 11 days ago)

I know this is an old thread but i want to put my input in.

From what i can tell from using the two, and i have been using both for almost 5 years now is that when you use rye, you tend to just have to soak for 24 hours, rinse and load and PC for 90 minutes and you're good.

WBS on the other hand, You soak for 24 hours, rinse, Start boiling a pot of water, when its boiling add WBS, stirring often reduce heat to a simmer until 15% of kernels have "popped" and turn off heat, rinse AGAIN, strain for 1 hour, then load, PC 90 minutes. I have an extremely low contam rate for WBS doing it this way and i use no hoods, gloveboxes, or anything for grains, only agar work etc. 

I personally like rye because its faster prep time, just a bit pricier.

And thats an outrage to say that grains affect potency, especially when they are used as spawn. Your substrate from my experience has been where the potency is coming from, and if you're going for potency go for poo.

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OfflineBrennus
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Re: RYE. VS. WBS [Re: 3500lt]
    #10012682 - 03/21/09 09:10 AM (15 years, 11 days ago)

Quote:

3500lt said:
well i have experience with both now. and i seem to have alot more contamination with WBS.. i dont know why. i prepare my WBS the same as the Rye berries. soak for 24 hours, strain, rinse, and pc for 1 hour at 15psi. with the Rye my contamination ratio is around 15% when i do 90quart jars. i have done 2 rounds with the WBS and i feel im waisting my time and money with it. Its alot of work to be throwing 75% of my birdseed away, and i am not doing anything different than with the rye. I do grain transfers right now, until my lc`s are ready, and i know i am giving a little room for contams by doing that, but 75% is rediculous. I lysol alot and the jars are not open for more than a couple seconds. i dont understand. im still new and learning like everyone else here, but its frustrating when your waisting that much time preparing all that spawn for nothing.




Are you doing open-air inoculations? Because that would explain your problem. Buy a $10 sterlite tote from Walmart and cute arm holes in it. Your contamination rates, if you flame sterilize your syringes and use latex gloves/tyvek wrist sleeves wiped down in alcohol, will be drastically reduced.

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Offlinerizingfire
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Re: RYE. VS. WBS [Re: Brennus]
    #10012742 - 03/21/09 09:31 AM (15 years, 11 days ago)

I was having problems with contamns when I used wbs so I started PCing for 2 hrs instead of just 60 min and found my problems went away...I definitely would make a glove box to inoculate in.....you'd be surprised how easily spores can get in while you are noccing it up

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OfflineRedDevil420
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Registered: 01/03/09
Posts: 1,079
Last seen: 10 years, 7 months
Re: RYE. VS. WBS [Re: 3500lt]
    #10012905 - 03/21/09 10:21 AM (15 years, 11 days ago)

Quote:

3500lt said:
Lol yea that makes sense. im doing a few different clones with the lc,s and they look good so far. had to clone the PE because i cant get any prints off of them. but yea im goona build a nice flow hood. thanks for the advice on pcing. 1.5 hours sounds good to me. cant believe i have done so many jars and not pc'd them long enough



You can clone directly to grains if I'm not mistaken.


--------------------
I'm Just Making This All Up.
Grains In Glass Jars With Professional Filters FTW.
Coir Monotubs FTW.
Sterile Technique FTW.
Fan dry, Desiccant storage FTW.
Ralphster's Spores FTW.

Mushroom Cloud - Dirtball

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OfflineRogerRabbitM
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Registered: 03/26/03
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Re: RYE. VS. WBS [Re: RedDevil420]
    #10013675 - 03/21/09 12:46 PM (15 years, 11 days ago)

He hasn't even logged in to the site in over ten months, so there's no point in trying to answer his questions.  His overwhelming failure rate indicated he was cutting corners on sterile procedure.  I'm assuming he gave up the hobby due to the work required to succeed.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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