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OfflineArthurSalenez
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Registered: 10/23/08
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Last seen: 14 years, 1 month
Does this happen to anyone?
    #9911522 - 03/04/09 04:16 PM (15 years, 18 days ago)

I used to syringe that I bought from a friend, said he took some spores from two mushroom caps and made it into one full shot. I inoculated 6 jars and only two came out developing mycelium. Anyone have a clue?

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Offlinemadwire3
queen of the smurfs
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Registered: 01/20/09
Posts: 522
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9911548 - 03/04/09 04:20 PM (15 years, 18 days ago)

wow too much info.. sure everyones had some jars grow slower than others.. the spore solution I'm using is made up of two prints from my B+ grow. works fine two prints to 1pint of water


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: madwire3]
    #9912013 - 03/04/09 05:32 PM (15 years, 18 days ago)

I have the penis envy spore syringe.

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Offlinemadwire3
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Registered: 01/20/09
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9912133 - 03/04/09 05:52 PM (15 years, 18 days ago)

how about u add more info like how long its been? how much solution u used?


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineArthurSalenez
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Registered: 10/23/08
Posts: 551
Last seen: 14 years, 1 month
Re: Does this happen to anyone? [Re: madwire3]
    #9912293 - 03/04/09 06:30 PM (15 years, 18 days ago)

Quote:

madwire3 said:
how about u add more info like how long its been? how much solution u used?




Wow, testy testy!

It's a brand new syringe and I used almost all of it on my 6 jars. I have 2 CCs left.

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Offlinemadwire3
queen of the smurfs
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Registered: 01/20/09
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9912309 - 03/04/09 06:32 PM (15 years, 18 days ago)

not testy just can't help when u are so breif... again more info how long has it been since u inoculated


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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Offlinelittleapplemm
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Registered: 01/14/09
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9912414 - 03/04/09 06:56 PM (15 years, 18 days ago)

Quote:

ArthurSalenez said:
It's a brand new syringe and I used almost all of it on my 6 jars. I have 2 CCs left.




2 cc's left of a 10 or 12 cc syringe? That seems like a lot of inoculant per jar to me...


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Taco Bell tastes better with bike grease!
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Invisibleshroomzey
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Re: Does this happen to anyone? [Re: littleapplemm]
    #9912568 - 03/04/09 07:26 PM (15 years, 17 days ago)

You need to describe your entire process.  What did you inooculate?  What size jars?  How did you sterilize them?  For how long? 

It's like coming to a doctor and saying," I hurt."  And thats it.

He can't do anything until you tell him what happened, where, what you hit, all the details.


--------------------

200 years from now, we will look back and laugh at ourselves for how stupid we were.
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Find a respected member of the community and study them.  I give thanks to: RogerRabbit, agar, hyphae, Nibin, fahtster, The shroomy 1, monstermitch, FooMan, HippieChick, Blue Helix, eatyualive, mycofile, and many, many more.
Never accept mediocrity, always return the love that is given to you.

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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: shroomzey]
    #9914378 - 03/04/09 11:51 PM (15 years, 17 days ago)

Quote:

shroomzey said:
You need to describe your entire process.  What did you inooculate?  What size jars?  How did you sterilize them?  For how long? 

It's like coming to a doctor and saying," I hurt."  And thats it.

He can't do anything until you tell him what happened, where, what you hit, all the details.




Lol, I hurt. lol

I don't expect a diagnosis. However, I wanted to ask a simple question that relied upon simple answers. Like a yes or no. You all are dodging it.

My original question was: Does this happen to anyone?

Rephrasing: Does this happen to anyone on a regular basis during cultivation?

But if you dying to know the exact details:

pf Tek jars
I inoculated jars
I Boiled them for 1 hour


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OfflineArthurSalenez
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Re: Does this happen to anyone? [Re: littleapplemm]
    #9914413 - 03/04/09 11:58 PM (15 years, 17 days ago)

Quote:

littleapplemm said:
Quote:

ArthurSalenez said:
It's a brand new syringe and I used almost all of it on my 6 jars. I have 2 CCs left.




2 cc's left of a 10 or 12 cc syringe? That seems like a lot of inoculant per jar to me...




I used a quarter of one CC for each hole (each jar has a total of 4 holes) for the top lid.

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Offlinemadwire3
queen of the smurfs
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Registered: 01/20/09
Posts: 522
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Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914418 - 03/05/09 12:00 AM (15 years, 17 days ago)

haha k my answer is blue...  good luck being a ass


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineMyName
Stranger

Registered: 02/06/09
Posts: 6
Last seen: 15 years, 13 days
Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914423 - 03/05/09 12:00 AM (15 years, 17 days ago)

Okay is that in order?  you inoculated then boiled?  Think you just told us what went wrong.

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OfflineArthurSalenez
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Registered: 10/23/08
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Re: Does this happen to anyone? [Re: madwire3]
    #9914429 - 03/05/09 12:02 AM (15 years, 17 days ago)

Quote:

madwire3 said:
haha k my answer is blue...  good luck being a ass




No problem, butch. :cool:

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Offlinemadwire3
queen of the smurfs
Female

Registered: 01/20/09
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Re: Does this happen to anyone? [Re: MyName]
    #9914436 - 03/05/09 12:03 AM (15 years, 17 days ago)

no no don't try to break down what's wrong he doesn't want help


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineArthurSalenez
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Registered: 10/23/08
Posts: 551
Last seen: 14 years, 1 month
Re: Does this happen to anyone? [Re: MyName]
    #9914451 - 03/05/09 12:06 AM (15 years, 17 days ago)

Quote:

MyName said:
Okay is that in order?  you inoculated then boiled?  Think you just told us what went wrong.




Of course not, i boiled then inoculated. But this topic is not about what went wrong, but why mycelium is only developing in 2 out of 6 jars.

Possibilities:

The more spores the faster the mycelium growth.

Or other unknown reasons.

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OfflineArthurSalenez
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Registered: 10/23/08
Posts: 551
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Re: Does this happen to anyone? [Re: madwire3]
    #9914457 - 03/05/09 12:07 AM (15 years, 17 days ago)

Quote:

madwire3 said:
no no don't try to break down what's wrong he doesn't want help




Please leave.

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OfflineMuffinlad
the hippie

Registered: 12/19/08
Posts: 110
Last seen: 12 years, 1 month
Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914462 - 03/05/09 12:08 AM (15 years, 17 days ago)

Quote:

ArthurSalenez said:

I used a quarter of one CC for each hole (each jar has a total of 4 holes) for the top lid.




1/4 of 1CC? Thats not good, should have atleast 1CC for each inoculation point. I inoculated 3/4 holes on one of my jars with uneven amounts and the last hole got less than 1CC and hasn't shown any signs of growth from that point. I inoculated on 02/22, all other jars are 90% colonized.


--------------------

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Offlinemadwire3
queen of the smurfs
Female

Registered: 01/20/09
Posts: 522
Last seen: 14 years, 10 months
Re: Does this happen to anyone? [Re: ArthurSalenez]
    #9914467 - 03/05/09 12:10 AM (15 years, 17 days ago)

u started it I was trying to help but what u don't get is in order to figure out why theres no myc people need some info u got butt hurt when I was trying to help. act like that and no one will help u here


--------------------
I agree totally. However, this forum isn't for the experienced growers, because they already know the ropes.  It's for the new growers who are just starting out.  Sometimes the hardest part about learning something new is un-learning bad info.  That's why I tend to correct people for calling a substrate tray a 'casing', etc.
RR

everything I say is a lie on here.


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OfflineMuffinlad
the hippie

Registered: 12/19/08
Posts: 110
Last seen: 12 years, 1 month
Re: Does this happen to anyone? [Re: madwire3]
    #9914479 - 03/05/09 12:12 AM (15 years, 17 days ago)

Quote:

madwire3 said:
u started it I was trying to help but what u don't get is in order to figure out why theres no myc people need some info u got butt hurt when I was trying to help. act like that and no one will help u here




True dat.


--------------------

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OfflineArthurSalenez
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Registered: 10/23/08
Posts: 551
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Re: Does this happen to anyone? [Re: Muffinlad]
    #9914482 - 03/05/09 12:13 AM (15 years, 17 days ago)

Quote:

Muffinlad said:
Quote:

ArthurSalenez said:

I used a quarter of one CC for each hole (each jar has a total of 4 holes) for the top lid.




1/4 of 1CC? Thats not good, should have atleast 1CC for each inoculation point. I inoculated 3/4 holes on one of my jars with uneven amounts and the last hole got less than 1CC and hasn't shown any signs of growth from that point. I inoculated on 02/22, all other jars are 90% colonized.




I was afraid that introducing more liquid into the jar would stimulate bacterial growth, according to what another forum member told me.
Would it be okay to use up the remaining 2 ccs on just one jar? Or is that too much? Thanks for understanding the question.

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