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 billycorgan55
Stranger
Registered: 11/14/08
Posts: 537
Last seen: 11 years, 11 months
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 is my liquid culture contaminated? 
#9760313 - 02/08/09 01:26 PM (15 years, 2 months ago) |
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I inoculated this jar yesterday afternoon and after about 20 hours of incubation at around 75-80 degrees it looks like this...I used a spore print and it has no odor...is this a contam?
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 Peterthinks
(Caulking) gun for hire
Registered: 11/10/04
Posts: 2,379
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Re: is my liquid culture contaminated? [Re: billycorgan55]
#9760337 - 02/08/09 01:32 PM (15 years, 2 months ago) |
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Looks fine to me, but only way to be sure is to test it on grain, BRF or agar.
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Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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billycorgan55
Stranger
Registered: 11/14/08
Posts: 537
Last seen: 11 years, 11 months
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Re: is my liquid culture contaminated? [Re: Peterthinks]
#9760392 - 02/08/09 01:41 PM (15 years, 2 months ago) |
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do u think it is normal for a lc to colnize that fast?
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houdinihar
Adventurous Learner
Registered: 09/12/08
Posts: 1,111
Last seen: 4 years, 8 days
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Re: is my liquid culture contaminated? [Re: billycorgan55]
#9760624 - 02/08/09 02:25 PM (15 years, 2 months ago) |
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Peterthinks,
thank you for this link--You can get 50 MM Polypropylene petri dishes HERE
http://www.tedpella.com/embed_html/36120.htm#petri_dish
absolutely appreciated. i have been looking for something like this and will buy some this week.
houdinihar
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Boris
Viking Warrior, of Valour
Registered: 08/28/08
Posts: 2,225
Loc: TX. 
Last seen: 1 year, 5 months
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Re: is my liquid culture contaminated? [Re: houdinihar]
#9760776 - 02/08/09 02:58 PM (15 years, 2 months ago) |
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Its not normal to have that much growth in one day from Cube mycelium. Its gotta be a contam. plus cube myc doesn't look like that unless you shake the Shit out of it, but even then, its hard to get small chunks like that. That looks like a bunch of sediment.
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AMU 
Terence McKenna Said:
"We have to create culture, don't watch TV, don't read magazines, don't even listen to NPR. Create your own roadshow. The nexus of space and time where you are now is the most immediate sector of your universe, and if you're worrying about Michael Jackson or Bill Clinton or somebody else, then you are disempowered, you're giving it all away to icons, icons which are maintained by an electronic media so that you want to dress like X or have lips like Y. This is shit-brained, this kind of thinking. That is all cultural diversion, and what is real is you and your friends and your associations, your highs, your orgasms, your hopes, your plans, your fears. And we are told 'no', we're unimportant, we're peripheral. 'Get a degree, get a job, get a this, get a that.' And then you're a player, you don't want to even play in that game. You want to reclaim your mind and get it out of the hands of the cultural engineers who want to turn you into a half-baked moron consuming all this trash that's being manufactured out of the bones of a dying world."
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Buckeye Oysters
From Zero to Hero
Registered: 08/09/08
Posts: 1,849
Last seen: 9 years, 4 months
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Re: is my liquid culture contaminated? [Re: Boris]
#9761052 - 02/08/09 03:51 PM (15 years, 2 months ago) |
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Well, doesn't look like bacteria, so I am thinking that it is alright, but you will have to test and see. LC's have to be proven clean because it is nearly impossible to tell if contam is present other than a greasiness from bacteria. That is a bit fast for spores to ferment, but not improbable.
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Evolution is Lamarckism in disguise. Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated. For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.
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messerschmied01
Chrono.naut
Registered: 01/18/09
Posts: 85
Loc: Near the Arctic Circle
Last seen: 4 years, 2 months
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Re: is my liquid culture contaminated? [Re: Boris]
#9761067 - 02/08/09 03:56 PM (15 years, 2 months ago) |
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How did you go about sterilizing your jars? It's hard to believe that myc. growth would occur so quickly, which means that it's probably contamination. If you were able to provide a little more info on your procedure, it might help you to get an answer.
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All that we see or seem, is but a dream within a dream.
When you're down, you stay down.
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Peterthinks
(Caulking) gun for hire
Registered: 11/10/04
Posts: 2,379
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Re: is my liquid culture contaminated? [Re: houdinihar]
#9761510 - 02/08/09 05:13 PM (15 years, 2 months ago) |
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Quote:
houdinihar said:
Peterthinks,
thank you for this link--You can get 50 MM Polypropylene petri dishes HERE
http://www.tedpella.com/embed_html/36120.htm#petri_dish
absolutely appreciated. i have been looking for something like this and will buy some this week.
houdinihar
No problem! enjoy the dishes, they're really nice!
let everything cool nice and slow in the PC if you cook them with agar inside. If you cool them rapidly they will deform. If you treat them nice they last forever.
And on topic I've seen LC to LC go that fast but not spores, didn't read it too carefull just looked at the pic. Might be good though, nothing obviously a contam.
test it.
--------------------
Give a man a fire and he will be warm for the rest of the night.
Set a man on fire and he will be warm for the rest of his life.
NEWB NEWBIE NEWCOMER IGNORANT? QUESTIONS?
Click HERE HERE HERE HERE For detailed instructions with pictures on how to grow mushrooms. There is a lot of info on the Shroomery and this is what you need to know.
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: is my liquid culture contaminated? [Re: Peterthinks]
#9761540 - 02/08/09 05:16 PM (15 years, 2 months ago) |
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Just my $0.02, I grew a nice trich LC. The myc looked just like mushroom myc up until it sporulated green all over the grain in my jars.
It grew probably 2x as fast as mushroom myc. Moral of the story is always test your LCs! (Oh, and you can't tell the difference between the myc sometimes)
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Premedman1
Assistant to the insistent
Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: is my liquid culture contaminated? [Re: billycorgan55]
#9761581 - 02/08/09 05:22 PM (15 years, 2 months ago) |
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Whatever it is, that's not mycelium.
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Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: is my liquid culture contaminated? [Re: Premedman1]
#9761597 - 02/08/09 05:24 PM (15 years, 2 months ago) |
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Quote:
Premedman1 said:
Whatever it is, that's not mycelium.
Looks like mine after a run on the magnetic stirrer.
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Premedman1
Assistant to the insistent
Registered: 12/21/05
Posts: 2,376
Loc: South of Sanity
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Re: is my liquid culture contaminated? [Re: Shroominit]
#9761611 - 02/08/09 05:26 PM (15 years, 2 months ago) |
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There's no way mycelium would grow that much in less than a day, especially from a spore inoculation. I can guarantee you that.
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Build a man a fire, he is warm for the night. Set a man on fire, he is warm for the rest of his life.
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: is my liquid culture contaminated? [Re: Premedman1]
#9761639 - 02/08/09 05:30 PM (15 years, 2 months ago) |
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Oh crap, you're right.
One day, that's not mushroom myc.
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billycorgan55
Stranger
Registered: 11/14/08
Posts: 537
Last seen: 11 years, 11 months
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Re: is my liquid culture contaminated? [Re: Boris]
#9762040 - 02/08/09 06:40 PM (15 years, 2 months ago) |
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This was after i shook it...sorry to leave that out...it was boiled sterilized for 20 mins...then inoculated the next day in a glovebox...
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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Re: is my liquid culture contaminated? [Re: billycorgan55]
#9762094 - 02/08/09 06:53 PM (15 years, 2 months ago) |
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Quote:
billycorgan55 said:
This was after i shook it...sorry to leave that out...it was boiled sterilized for 20 mins...then inoculated the next day in a glovebox...
It's probably contaminated.
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billycorgan55
Stranger
Registered: 11/14/08
Posts: 537
Last seen: 11 years, 11 months
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Re: is my liquid culture contaminated? [Re: Shroominit]
#9762344 - 02/08/09 07:42 PM (15 years, 2 months ago) |
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yy? because of the boil sterilization?
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messerschmied01
Chrono.naut
Registered: 01/18/09
Posts: 85
Loc: Near the Arctic Circle
Last seen: 4 years, 2 months
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Re: is my liquid culture contaminated? [Re: billycorgan55]
#9762492 - 02/08/09 08:08 PM (15 years, 2 months ago) |
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You just didn't boil it nearly long enough to fully sterilize it. 20 minutes is not at all sufficient. You generally need over an hour, but every grower has their own preference as to how long it should be done. Experiment and see what works for you. I hope this helped.
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All that we see or seem, is but a dream within a dream.
When you're down, you stay down.
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Shroominit
Part Time Mycologist
Registered: 11/29/08
Posts: 4,662
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I wouldn't trust boiling at all. For any length of time.
I PC or autoclave. No exceptions.
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Basement Boy
Only two degrees of separation
Registered: 11/06/08
Posts: 580
Last seen: 14 years, 10 months
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You don't do a LC for an hour. 20 should be fine from the tek's I've seen. You can't really know until you test it so give it a go and see what you have.
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Don't Panic It's Organic 
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kami
newbie grow
Registered: 02/02/09
Posts: 284
Last seen: 15 years, 2 days
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Re: is my liquid culture contaminated? [Re: Basement Boy]
#9762527 - 02/08/09 08:14 PM (15 years, 2 months ago) |
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would you just test it on a prepared substrate? or is there a way to test without possibly wasting ur jars
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When reading my posts pls refer to my title. A large amount of my knowlege is from this site. I mean to lead no1 a stray
I in no way shape or form partake in any sort of illegal activity, anything i post, say or do in this forum is completley hypothetical and is my way of exercising my right to FREE SPEECH. first amendment biatch
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