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Offlineshroombab
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Grain Jar injection holes and open air inoculation questions
    #9616645 - 01/15/09 06:37 PM (15 years, 2 months ago)

I've scoured the boards and haven't found my answer so I'm going to post - excuse me if I missed a post with the answer.

My glovebox idea didn't work out as attempting to cut holes in a clear rubbermaid just resulted in cracking and shattering sharp holes. so, I used another smaller clear rubbermaid, lysol'd the shit out of it, and worked in there in open air, in a small corner of a room, that I also lysol'd the shit out of, and moved everything else at least 10 feet away, barely moved around, used a fresh cover all on top of freshly laundered clothes, and a sterile mask. and sterile gloves, phew.

I have two layers of tyvek over top of the metal lid with three small holes. Jars came directly out of unopened PC cooled over night in sterile room. Problem #1 is - the needle didn't fit through the holes. After opening the bags and putting the needle on I flamed for a few second and went straight in, but had to wiggle around to get it in - worried that might of caused problems. Jars then went straight into extremely clean cooler incubator, with heat blanket on the inside wall. That had also been lysol'd to death previously. I inoculated as quickly as humanly possible and placed the jars into the cooler (without taping the holes or covering with foil) and closed the lid of the cooler.

Now its a waiting game - but I'm wondering if either of the hole size or non-taping will become an issue. On the up side - its only two jars, as thats all that fits in the PC at one time. I'm doing another two which I have modified the holes under the tyvek, and thinking of taping the injection site one with a band aid, and then compare the two batches. Also shook the jars a little just after throwing into incubator to spread spores around. and put about 1.5cc in each 1 litre jar for good measure.

I will have to open the cooler again to put the other jars in, and to check that the temp is ok, but plan to keep it closed for the most part. and will lysol the area and wait a while each time before opening, also wearing a mask and minimizing anything that might go in.

Think I did everything right. Any thoughts? critiques?

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Offlinejohnny_poke
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Registered: 12/03/08
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Re: Grain Jar injection holes and open air inoculation questions [Re: shroombab]
    #9616735 - 01/15/09 06:50 PM (15 years, 2 months ago)

Quote:

shroombab said:
that I also lysol'd the shit out of






  You paint a picture :hehehe:


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[quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]

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OfflineRogerRabbitM
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Re: Grain Jar injection holes and open air inoculation questions [Re: johnny_poke]
    #9616759 - 01/15/09 06:54 PM (15 years, 2 months ago)

Lysol is a surface disinfect and doesn't do squat for the air, which is what you need to be worried about.  If you can't cut holes in the plastic tote, simply lay the tub on its side and cut a piece of plastic sheeting to cover the opening, and you can easily cut arm holes in that.
RR


--------------------
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Thomas Edison

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Offlineshroombab
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Re: Grain Jar injection holes and open air inoculation questions [Re: RogerRabbit]
    #9616780 - 01/15/09 06:59 PM (15 years, 2 months ago)

Let me add that I wiped, then cleaned, then disinfected, then lysol wiped, and then sprayed lysol, onto everything, and the entire area, ah I did the best I could with what I had to work with. The glove box situation can easily be rectified by tomorrow, I'm wondering about the hole covering (or not) and the issue with having to wiggle the needles in through the holes.

RR I used your instructions by the way. Everything except the glovebox part which failed miserably. Incidentally, how do you cut holes in plastic without it shattering?

: )

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OfflineLennyk
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Re: Grain Jar injection holes and open air inoculation questions [Re: shroombab]
    #9616818 - 01/15/09 07:05 PM (15 years, 2 months ago)

Quote:

shroombab said:
Let me add that I wiped, then cleaned, then disinfected, then lysol wiped, and then sprayed lysol, onto everything, and the entire area, ah I did the best I could with what I had to work with. The glove box situation can easily be rectified by tomorrow, I'm wondering about the hole covering (or not) and the issue with having to wiggle the needles in through the holes.

RR I used your instructions by the way. Everything except the glovebox part which failed miserably. Incidentally, how do you cut holes in plastic without it shattering?

: )




I said hell with being gentle after about 20 minutes of delicate work caused it to crack. I just stabbed the heck out of it and taped up all the cracks.

On a side not, I thought the purpose of a glove box was not sterile air, just not moving air?


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Offlinejohnny_poke
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Registered: 12/03/08
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Re: Grain Jar injection holes and open air inoculation questions [Re: shroombab]
    #9616826 - 01/15/09 07:07 PM (15 years, 2 months ago)

I know I know!!! :gameover:

Get a bumper size can of tomatos/whatever, eat the contents and then heat the rim of the can on your stove and proceed to burn a perfect circular hole in your box :smile:

I deserve a gold star I think :smile:


--------------------
[quote]But what our scientist does not realize is that every time he makes a measurement, the Flying Spaghetti Monster is there changing the results with His Noodly Appendage.[/quote]

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Offlineshroombab
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Re: Grain Jar injection holes and open air inoculation questions [Re: johnny_poke]
    #9616861 - 01/15/09 07:14 PM (15 years, 2 months ago)

I was thinking about that....gold star fo sho

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OfflineRogerRabbitM
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Re: Grain Jar injection holes and open air inoculation questions [Re: shroombab]
    #9616877 - 01/15/09 07:16 PM (15 years, 2 months ago)

Quote:

shroombab said:
Incidentally, how do you cut holes in plastic without it shattering?

: )





--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Offlineshroombab
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Registered: 01/15/09
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Re: Grain Jar injection holes and open air inoculation questions [Re: RogerRabbit]
    #9616897 - 01/15/09 07:19 PM (15 years, 2 months ago)

RR - why are you so smart?

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Offlineshroombab
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Re: Grain Jar injection holes and open air inoculation questions [Re: shroombab]
    #9618550 - 01/15/09 11:58 PM (15 years, 2 months ago)

hmm, just did the 2nd two jars. this time, put the foil back on and straight into the incubator, although when I checked the temp it was 93F oops. I guess this is going to be a trial and error despite my 5+ years of reading on the subject. at least its only 4 jars.
oh and I took two needles out of their bags and forgot which one was which. dum dum.

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InvisibleShroominit
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Registered: 11/29/08
Posts: 4,662
Re: Grain Jar injection holes and open air inoculation questions [Re: shroombab]
    #9619004 - 01/16/09 01:38 AM (15 years, 2 months ago)

You don't need an incubator, and I would recommend taping the holes (micropore) and not using the foil as it doesn't allow for much GE which is pretty important to your colonization.

Also the coffee can thing was just amazing. I've also used a hole saw but sometimes they will crack plastic, if you use cheapo plastic tubs and have a hole saw, run it backwards. :wink:

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InvisibleGerman Kahuna
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Re: Grain Jar injection holes and open air inoculation questions [Re: shroombab]
    #9619067 - 01/16/09 01:59 AM (15 years, 2 months ago)

Quote:

shroombab said:
RR - why are you so smart?




<yoda> When 900 years you grow, make n00b mistakes you will not, hmmm </yoda> :wink:


--------------------
"Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".

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OfflinePandeist
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Re: Grain Jar injection holes and open air inoculation questions [Re: German Kahuna]
    #9619100 - 01/16/09 02:09 AM (15 years, 2 months ago)

Don't forget tothe fuck out of the air as well.

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