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Offlinemycelialfruit
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Agar...Brand
    #9596404 - 01/12/09 05:21 PM (15 years, 2 months ago)

now brand agar seems a bit cheper....worth buying instead of bacteriological grade?  seen the lit.  just want personal experience.

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InvisibleFooMan
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Re: Agar...Brand [Re: mycelialfruit]
    #9597048 - 01/12/09 06:48 PM (15 years, 2 months ago)

I'm not sure what "brand" agar is, but you don't need lab grade. All I've ever used is food grade agar and it works great.

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Offlinemycelialfruit
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Re: Agar...Brand [Re: FooMan]
    #9597134 - 01/12/09 07:04 PM (15 years, 2 months ago)

Thanks....NOW is the brand of food grade I am speaking of.  $50 for 908 grams compared with ~$40 for 500 grams of bacteriological grade

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OfflineRogerRabbitM
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Re: Agar...Brand [Re: mycelialfruit]
    #9597160 - 01/12/09 07:08 PM (15 years, 2 months ago)

Make a small batch first to test it.  Some of the food grade agar needs to be boiled to dissolve it first, and then cooled to add the malt, etc., for nutrient in order to prevent clumping.  With lab grade, I mix it all up, add cold water, stir well, and PC.
RR


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Invisiblefastfred
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Re: Agar...Brand [Re: RogerRabbit]
    #9610621 - 01/14/09 07:39 PM (15 years, 2 months ago)

Food grade has worked plenty well for me.  It actually seems to have a great gel strenght.  I mix a fair amount of 'soft' agar and food grade gells down to concentrations as low as lab grade.


-FF

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InvisiblePremedman1
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Re: Agar...Brand [Re: fastfred]
    #9623850 - 01/16/09 10:45 PM (15 years, 2 months ago)

All I've ever used is NOW brand with good results.


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InvisibleFooMan
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Re: Agar...Brand [Re: Premedman1]
    #9633069 - 01/18/09 05:13 PM (15 years, 2 months ago)

Quote:

Premedman1 said:
All I've ever used is NOW brand with good results.




Good to know. I've seen that stuff a few times and wondered how well it would work. I'll probably pick some up next time.

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Invisiblepoboy
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Re: Agar...Brand [Re: FooMan]
    #9660220 - 01/22/09 05:02 PM (15 years, 2 months ago)

I buy from a Chinese store 45 grams for around two bucks works great.10 grams is plenty for 20 plates


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OfflineCultosaurus
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Re: Agar...Brand [Re: RogerRabbit]
    #9667012 - 01/23/09 06:40 PM (15 years, 2 months ago)

Quote:

RogerRabbit said:
Make a small batch first to test it.  Some of the food grade agar needs to be boiled to dissolve it first, and then cooled to add the malt, etc., for nutrient in order to prevent clumping.  With lab grade, I mix it all up, add cold water, stir well, and PC.
RR




So that's what happened to mine. I just added it to the potato water, it came out like lumpy gravy. Thanks, I'll try that the next time.


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Invisibletahoe
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Re: Agar...Brand [Re: Cultosaurus]
    #9702367 - 01/29/09 08:52 PM (15 years, 1 month ago)

i have used "now" brand and it works just fine


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InvisibleDuaut
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Re: Agar...Brand [Re: tahoe]
    #9707032 - 01/30/09 05:37 PM (15 years, 1 month ago)

Now is the bomb. very cheap and easy to work. just made some PDA yesterday
20g NOW agar
200g potato
dash of honey, karo, and  dextrose

makes enough for 2 500ml jars

I cook the potato's for an hour, then strain, add sugars to potato water and then add agar, do a low boil for 15min, then pour into jars, and then PC at 15lbs for 1hr


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Offlinefishfarmin
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Re: Agar...Brand [Re: Duaut]
    #9708909 - 01/30/09 10:14 PM (15 years, 1 month ago)

i have always just went to the local asian market for all my agar.if you can find "telephone" brand its ground to a powder.its also under 2$ for an oz.it easy to mix with your favorite additives and water cause of the powder it ground to.which i do prior to sterilizing and i've always had perfectly smooth finishes to my cultures.i tried the cornstarch method this year for the first time,and have since gone back to agar.not knockin cornstarch,just cant stand the lumps.                                      :2cents:


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"Government exist to protect us from each other.Where government has gone beyond its limits is in deciding to protect us from ourselves."    Ronald Reagan

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Offlinemakaveli8x8
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Re: Agar...Brand [Re: fishfarmin]
    #9715994 - 02/01/09 07:08 AM (15 years, 1 month ago)

seems like jello would work if your really patient to let it grow out in the fridge lol


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OfflineWhAcKeD
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Re: Agar...Brand [Re: makaveli8x8]
    #9741031 - 02/05/09 02:17 PM (15 years, 1 month ago)

I use the agar from the asian food store, $1.19 a packet works out to 1200 grams for $50, that's the best deal I've found and never had a problem.

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Offlinebw86
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Re: Agar...Brand [Re: WhAcKeD]
    #9778158 - 02/11/09 01:21 PM (15 years, 1 month ago)

i don't mean to thread jack but i was wondering what the deal is with  these agars and if anyone has any experience with them they are considerably more expensive then other agars and i didn't know if they were worth it for our hobby in any way they are dehydrated grams.
m-endo agar 500g/bottle $103.00 (USD) a search on the stuff had things like " For the enumeration of coliforms in water using the Membrane filter technique for coliform enumeration." i am not that far into this that i know what it means.
then there is Brain heart infusion agar, 500 g jar $90.00 (USD)-  not much info on this
there are a few others that are not much more expensive but the original questions still apply
Sabouraud Dextrose Agar, 500 g $45.50 (USD for this i found
A dextrose peptone media that supports the growth of most pathogenic fungi. i looked more into that and it seems like it would be good
and Tryptic soy agar, 500 g jar $53.05 (USD) i know that someone at the shroomery used this im about to do a search on it now.
so anyone with any experience with these and if they had any comment they wanted to add.
thanks in advance

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Invisiblefastfred
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Re: Agar...Brand [Re: bw86]
    #9778508 - 02/11/09 02:17 PM (15 years, 1 month ago)

Those are medias.  They are agar + nutrients.  This thread is only about agar prices.  Nobody uses those prepared medias here because they are so damn expensive and you can make all the useful types of media yourself from cheap agar and cheap nutrients.


-FF

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Offlinebw86
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Re: Agar...Brand [Re: fastfred]
    #9778574 - 02/11/09 02:27 PM (15 years, 1 month ago)

sorry ill make a new post after my first post is gone

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