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 k4ge
 
Registered: 11/25/08
Posts: 1,139
Last seen: 2 years, 5 months
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 Stunted Myc Growth, Suggestions/Comments. 
#9472782 - 12/21/08 01:27 PM (15 years, 3 months ago) |
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Alright, heres the facts:
Spores where injected into BRF Cakes November 28th in a glovebox that was placed into a well sterilized room. Incubated between 70-78 f with two nights having a temp drop to 65.
Dec 2nd, Myc spots formed on all 10 inoculated jars.
Dec 8th I noticed that the Myc development had slowed down a little, so I loosened lids a tad, growth continued in 1/2 of the jars.
Since then, the jars have just been... Crawling along, except for one jar that ROCKETED and has covered almost 1/2 of the cake while all others are very stunted.
Last night I flipped the jars, and place them on their sides today.
I also, noticed what looked like wet spots on the inside of the jar around the myc started developing but it hasnt gotten any bigger. The jars smell to me like Brown rice/leaves/mushrooms but my GF hates the smell.
So basically I am asking should I:
-Try and salvage the best jars myc by transfering it to a Liquid Culture?
-Leave it alone for another week.
-Put a little bit of H202 in there.
-Turf it, its no good. Try again next time.
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Brennus
Student of Life
Registered: 05/31/08
Posts: 3,297
Loc: SE United States 
Last seen: 6 years, 1 month
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: k4ge]
#9472878 - 12/21/08 01:42 PM (15 years, 3 months ago) |
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If the 'wet spots' developed after your sterilized your jars, you could have a bacterial contamination.
Dumping in H202 will cause a lot more problems than it would even begin to solve.
Did you put holes in the lids of the jars and do you have a vermiculite barrier?
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k4ge
Registered: 11/25/08
Posts: 1,139
Last seen: 2 years, 5 months
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: Brennus]
#9472934 - 12/21/08 01:51 PM (15 years, 3 months ago) |
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there are 4 holes in the lid, with tyvek ontop of the lid, then the ring on the jar.
Yes there is a verm barrier. 
The wet spots Have not gotten bigger since I noticed them, (early december)
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Edited by k4ge (12/21/08 01:51 PM)
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Brennus
Student of Life
Registered: 05/31/08
Posts: 3,297
Loc: SE United States
Last seen: 6 years, 1 month
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: k4ge]
#9472948 - 12/21/08 01:53 PM (15 years, 3 months ago) |
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For future reference, you don't need the tyvek if you have a vermiculite barrier. Dry vermiculite works just fine on its own.
It's possible the tyvek is hampering gas exchange if it's too thick in conjunction with the vermiculite. Ordinarily, I wouldn't say that's a problem, but it's a shot in the dark here because you seem to have done everything else right.
Personally, I would take off the tyvek and give your jars another week before starting over.
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Brennus
Student of Life
Registered: 05/31/08
Posts: 3,297
Loc: SE United States
Last seen: 6 years, 1 month
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: Brennus]
#9472958 - 12/21/08 01:55 PM (15 years, 3 months ago) |
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This is also assuming that you got your moisture content right when you mixed up your BRF Mix. I cut my teeth on 'The PF Tek for Simple Minds' and RR's PF Tek Videos (they're posed on mushroomvideos.com) when I first started cultivating and it worked perfect for me.
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k4ge
Registered: 11/25/08
Posts: 1,139
Last seen: 2 years, 5 months
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: Brennus]
#9473044 - 12/21/08 02:09 PM (15 years, 3 months ago) |
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so should I remove the tyvek, and put some microspore tape on the holes?
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Seriously.
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dead
grateful
Registered: 08/28/08
Posts: 2,761
Loc: North pole
Last seen: 9 years, 4 months
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: k4ge]
#9473109 - 12/21/08 02:27 PM (15 years, 3 months ago) |
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Remove the tyvek but don't bother taping holes. The dry verm is your filter.
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"The third eye. You spend years doing everything you can to open it and then the damn thing opens and your friends laugh at you when you tell them you can see their souls behind their eyes burning like rainbows."
Links:
 Nibin's Guide for Noobs
some easy teks on bulk & grain prep. (my journal)
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 Demonic_Chronic
The Plague Doctress
Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: dead]
#9473232 - 12/21/08 02:54 PM (15 years, 3 months ago) |
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Sounds to me like a bacterial contam. as Brennus said, loosen your lids a little bit and take a whiff if you smell fermentation like sour apples then toss em and try again. good luck.
DC
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The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
 
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k4ge
Registered: 11/25/08
Posts: 1,139
Last seen: 2 years, 5 months
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: Demonic_Chronic]
#9473609 - 12/21/08 04:16 PM (15 years, 3 months ago) |
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Quote:
Demonic_Chronic said:
Sounds to me like a bacterial contam. as Brennus said, loosen your lids a little bit and take a whiff if you smell fermentation like sour apples then toss em and try again. good luck.
DC
Quote:
k4ge said:
I also, noticed what looked like wet spots on the inside of the jar around the myc started developing but it hasnt gotten any bigger. The jars smell to me like Brown rice/leaves/mushrooms but my GF hates the smell.
After reading your reply Brennus I removed the tyvek, lets see how it is in a week!
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All posts by this user are not to be taken seriously.
Seriously.
Edited by k4ge (12/21/08 04:17 PM)
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Demonic_Chronic
The Plague Doctress
Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: k4ge]
#9474817 - 12/21/08 08:07 PM (15 years, 3 months ago) |
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haha didnt see that sorry.well ur jars could just be too wet.
DC
--------------------
The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
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Demonic_Chronic
The Plague Doctress
Registered: 08/10/08
Posts: 4,199
Loc: PNW
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: Demonic_Chronic]
#9474825 - 12/21/08 08:09 PM (15 years, 3 months ago) |
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also its just pf tek. id start new if i were u. thats just my $1/50
DC
--------------------
The Real violence, the violence that I realized was unforgiveable
Is the violence that we do to ourselves
When we are too afraid to be, who we really are.
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k4ge
Registered: 11/25/08
Posts: 1,139
Last seen: 2 years, 5 months
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Re: Stunted Myc Growth, Suggestions/Comments. [Re: Demonic_Chronic]
#9474928 - 12/21/08 08:29 PM (15 years, 3 months ago) |
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Quote:
Demonic_Chronic said:
also its just pf tek. id start new if i were u. thats just my $1/50
DC
Thats what I was thinking too.
I got some WBS and I am working on making my lids, going to mail my order to Ralphster tomorrow, so a new batch will be started soon anyways 
Just wanted to maybe get at least one cap for genetics, you know?
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All posts by this user are not to be taken seriously.
Seriously.
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