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skaterickets
Musician
Registered: 06/26/08
Posts: 653
Last seen: 9 years, 8 months
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Over Dunking = Cobweb
#9399516 - 12/09/08 01:24 PM (15 years, 3 months ago) |
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and I learned it the hard way.
I'm very careful about my dunking too. I fill a jar with boiling mineral water and put tyvek on top. Put it in the freezer for almost frozen cold water, and then quickly put in the cake, and close the lid.
I was very tired one night however and when I should've taken the cakes out at the 24hr line, I was asleep, woke up the next morning then quickly rolled and fruited, but on the third day they got cobweb and I've just been spraying them with H2O2 twice and some has gone away but not all.
I've got about 6 cakes contaminated and I really want to save them. I'm thinking of doing an H2O2 dunk, what do you guys think?
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German Kahuna
Facepalmer of Stoopid
Registered: 10/31/08
Posts: 15,798
Loc: On a Chemical Vacation
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Are you 100% sure that what you identified as cobweb aren't mycelium bumps? I had them right after fruiting some pf cakes and they looked like cottony balls and I first thought they were cobweb. Then I peeled one off to discover it was actually a ball of myc. The H2O2 should have taken care of it all if it was cobweb. Got pics?
-------------------- "Vegetarian" [ /ˌvedʒəˈteəriən/] - Ancient slang meaning "village idiot who can't hunt, fish or ride".
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Cascadian
Feel like a Stranger
Registered: 09/13/08
Posts: 1,130
Loc: Cascadia
Last seen: 12 years, 1 month
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boiling mineral water with tyvek?
Damn homie. Just use tap water. Throw a capful or two of H202 in the water if you're paranoid.
You didn't specify, but dont dunk your cakes in the refrigerator either. Cover with a plate and a jar of water.
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 1 month
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Doesn't sound like cobweb to me.. Also cobweb is from stail air.. I dunk my cakes in tap water in a pot for 12-24 hours then just stick them back in the FC
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skaterickets
Musician
Registered: 06/26/08
Posts: 653
Last seen: 9 years, 8 months
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Re: Over Dunking = Cobweb [Re: Damion5050]
#9399823 - 12/09/08 02:07 PM (15 years, 3 months ago) |
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Yes, I'm 100% sure this was cobweb. My terrarium does not have a lot of flowing air, I was thinking of transferring them to my shotgun terrarium where I grow my edibles.
I also know I over exaggerate a little when I dunk but was paranoid, the week after those I dunked 5 more cakes on filtered tap water for 24hrs and they're doing fine after 3 days.
I don't know what to do with those 5 other though. I really don't want to loose them. No thoughts on a H2O2 bath?
Where's RR or a TC when you need them.
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 1 month
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Quote:
skaterickets said: Yes, I'm 100% sure this was cobweb. My terrarium does not have a lot of flowing air, I was thinking of transferring them to my shotgun terrarium where I grow my edibles.
I also know I over exaggerate a little when I dunk but was paranoid, the week after those I dunked 5 more cakes on filtered tap water for 24hrs and they're doing fine after 3 days.
I don't know what to do with those 5 other though. I really don't want to loose them. No thoughts on a H2O2 bath?
Where's RR or a TC when you need them.
Well the problem I hear you said your FC doesn't have alot of flowing air.. That is your problem.. Just spray them with a spray bottle with H2O2 in it.. If it starts to go away it's cobweb.. Keep spraying it till it's all gone..
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FooMan
Registered: 02/02/05
Posts: 8,957
Loc: Earth
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Lack of FAE = cobweb, not dunking. Why did you put near freezing water on your cakes? The "cobweb" may just be some weak myc growth from freezing the shit out of the cake. Give it some time and DON'T dunk it in H2O2. If you've sprayed it with H2O2 and it didn't go away, it's not cobweb. Using more H2O2 will just slow you down more.
-------------------- Quick WBS Prep
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Damion5050
Mush Doctor
Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 1 month
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Re: Over Dunking = Cobweb [Re: FooMan]
#9399980 - 12/09/08 02:25 PM (15 years, 3 months ago) |
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What do you know I was partly right FAE is the problem...
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skaterickets
Musician
Registered: 06/26/08
Posts: 653
Last seen: 9 years, 8 months
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Re: Over Dunking = Cobweb [Re: FooMan]
#9400328 - 12/09/08 03:12 PM (15 years, 3 months ago) |
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Alright, I'll do some more H2O2 spraying and place them in a shotgun terrarium, thanks guys.
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Quote:
skaterickets said: Alright, I'll do some more H2O2 spraying and place them in a shotgun terrarium, thanks guys.
If the suspected cobweb did not dispear after spraying with h202 its not cobweb.
As shroomery1 said its probably weak mycelium from being subject to near freezing water for an extended period.
You dont need cold water for dunk. It will just slow things right down. Cool tap water is fine. If your tap water is drinkable its fine, no need to boil it.
Dont spray more h202 which will shock your mycelium more.
Pictures??
this is what cobweb looks like
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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J3illy
Trainee
Registered: 10/18/08
Posts: 3,344
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Re: Over Dunking = Cobweb [Re: veda_sticks]
#9400443 - 12/09/08 03:29 PM (15 years, 3 months ago) |
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H2O2 isn't the best idea as FooMan pointed out. Believe it or not, bleach at a ratio of 1:100 works good at killing/preventing any small amounts of contams you may have during dunking, or on the cake. So for 2 liters of water - that's 20cc of bleach, which is 4 teaspoons, or 1.25 tablespoons. There's actually been reports that some ppl claim that the bleach may actually help to promote fruiting. Someone said they got better yields from the substrates dunked in the bleach water, although it's not necessarily proof or anything. It is totally safe tho, and is perfect if you're worried about a contam during dunking.
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 4 years, 2 months
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Re: Over Dunking = Cobweb [Re: J3illy]
#9400476 - 12/09/08 03:34 PM (15 years, 3 months ago) |
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Quote:
J3illy said: H2O2 isn't the best idea as FooMan pointed out. Believe it or not, bleach at a ratio of 1:100 works good at killing/preventing any small amounts of contams you may have during dunking, or on the cake. So for 2 liters of water - that's 20cc of bleach, which is 4 teaspoons, or 1.25 tablespoons. There's actually been reports that some ppl claim that the bleach may actually help to promote fruiting. Someone said they got better yields from the substrates dunked in the bleach water, although it's not necessarily proof or anything. It is totally safe tho, and is perfect if you're worried about a contam during dunking.
I vaguely remember that experiment from last year. Like alot of experiments it was never followed up with more testing. Either that or the orginal experimenter disapeared.
I would use bleach before h202. Bleach can be used to clean up tissue samples for cloning. It doesnt hurt mycelium much.
However, when dunking i still find it unnessesary. fully colinised cakes are pretty resistant to contamination. After the dunk, rinsing under the tap should clean of any bacteria that may be pressent.
Generaly if your cake contaminates during fruting. It was probably already contaminated prior to dunking. Only other reason would be lack of fresh air. Contams love still stale air.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
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Re: Over Dunking = Cobweb [Re: veda_sticks]
#9401339 - 12/09/08 05:25 PM (15 years, 3 months ago) |
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Guys, bleach doesn't kill molds. I've dunked in 10% bleach before just to prove it. It will kill most mold spores, but not live mold(or mushroom) mycelium.
Use peroxide at 3% to kill cobweb mold. It has little other use, and bleach at 1:100 won't touch cobweb. 3% is the concentration that peroxide is sold at in the US in drug and grocery stores, so use the brown bottle peroxide full strength in your mister, but only if you see cobweb.
As said, air exchange is the way to prevent cobweb. It only thrives in stale, still air. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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