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OfflineFunguyparty
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Registered: 10/29/08
Posts: 17
Last seen: 15 years, 22 days
PC vs Microwave? Humidity in incubator?
    #9262361 - 11/17/08 07:44 PM (15 years, 4 months ago)

Hey friends,

Just wanted to get some general thoughts on Microwave sterilization vs PC or steam Sterilization. Never tried the microwave have you? what are your thoughts. Also Do you think the humidity in the incubator slows or takes away from the colonizations process?

Thanks

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Offlineveda_sticks
Cultivator
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Registered: 07/29/07
Posts: 14,191
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Last seen: 4 years, 2 months
Re: PC vs Microwave? Humidity in incubator? [Re: Funguyparty]
    #9262381 - 11/17/08 07:47 PM (15 years, 4 months ago)

That depends on what you sterilising. If your sterilising grains a pressure cooker is a must. Steam sterilising works fine for pf tek and LC's. I have never used the microwave to sterilise. Id stick with steaming/PC.

As for humidity in your incubator. You dont need Humidity during colinisation. High rh could get the filter damp which will alow contaminants to grow through your filter material.


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OfflineCporzl23
Stranger

Registered: 07/24/08
Posts: 27
Last seen: 10 years, 4 months
Re: PC vs Microwave? Humidity in incubator? [Re: veda_sticks]
    #9262626 - 11/17/08 08:27 PM (15 years, 4 months ago)

I'm pretty sure the microwave is widely agreed to be the most contam prone method of sterilization, although I could be wrong. I used boiling water in a pot to sterilize my wbs, worked out fine for the most part, although I can certainly see the benefits of using a pc.

Food for thought.

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Offlineanakin3197
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Registered: 08/21/08
Posts: 1,425
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Re: PC vs Microwave? Humidity in incubator? [Re: Cporzl23]
    #9263114 - 11/17/08 09:45 PM (15 years, 4 months ago)

ive read that some people only nuke casing materials. jus think about how nasty a mic can be. someone once said that a gnat flew in his nuker, and when his cycle was done it flew out unharmed. would that be something u would wanna trust?

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