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OfflineShroomer Smurf
Slacker


Registered: 11/02/08
Posts: 176
Last seen: 11 years, 11 months
Liquid Culture Questions?
    #9238264 - 11/13/08 04:05 PM (15 years, 4 months ago)

Im interested in starting with a liquid culture. Where do I get the large syringe and long tip to draw the liquid out? I have access to pet syringes but theyre to small I think. Also where do I get a cheap/good magnetic stirrer? How much liquid culture in each jar? Whats the best liquid culture tek?


Thanks In Advance. :stoned:

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OfflineMOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 6 months
Re: Liquid Culture Questions? [Re: Shroomer Smurf]
    #9238298 - 11/13/08 04:10 PM (15 years, 4 months ago)

Easy and cheap LC tek

Blank sterile syringes

Edit: for a stirrer you can just use a marble or piece of broken glass


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter

Edited by MOPE (11/13/08 04:11 PM)

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InvisibleJ3illy
Trainee

Registered: 10/18/08
Posts: 3,344
Re: Liquid Culture Questions? [Re: MOPE]
    #9238323 - 11/13/08 04:13 PM (15 years, 4 months ago)

I don't understand how Ralphster is charging $5 for 2 syringes, and then says "Yes it's true!" like it's some crazy deal, lol.  MadSeason has 5 for less than that - and 100 for $25 @ 25 cents each, which is what they really cost.

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OfflineMOPE
Walking Chemical Reaction


Registered: 10/07/08
Posts: 831
Last seen: 10 years, 6 months
Re: Liquid Culture Questions? [Re: J3illy]
    #9238366 - 11/13/08 04:19 PM (15 years, 4 months ago)

Quote:

J3illy said:
I don't understand how Ralphster is charging $5 for 2 syringes, and then says "Yes it's true!" like it's some crazy deal, lol.  MadSeason has 5 for less than that - and 100 for $25 @ 25 cents each, which is what they really cost.




damn yeah i always thought they were too expensive but i never found them much cheaper anywhere else, but if you do their blank special you get their strain of the month free:mushroom2:


--------------------
Got used to the feeling of falling
But you'll never see me following
Bouncing back and forth between the healing and the hollering
Riding the outer ring of my own private saturn
Thoughts scattered all across the grey matter

Edited by MOPE (11/13/08 04:19 PM)

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Offlinegethyn
MR.STINK FINGER
Male User Gallery


Registered: 03/03/08
Posts: 758
Loc: first row at church
Last seen: 9 months, 7 days
Re: Liquid Culture Questions? [Re: MOPE]
    #9238367 - 11/13/08 04:19 PM (15 years, 4 months ago)

I get my 60cc and 140 cc luker lock syringes at a local farm supply outlet they are for livestock and are sterile and cheap. TSC tractor supply or rural king is where i go  :cheers:


--------------------
I RESEMBLE THE REST

Edited by gethyn (11/13/08 04:20 PM)

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Offlinealkylbenzene23
Indeed
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Registered: 08/16/08
Posts: 615
Last seen: 14 years, 4 months
Re: Liquid Culture Questions? [Re: gethyn]
    #9238556 - 11/13/08 04:42 PM (15 years, 4 months ago)

Yea, Don't buy them from the mycology supply places. If you just need one or two, you can try asking your doctor- minewas willing.
I needed syringes+needles  long before I started mycology and the doc was like ok.(Solvent welding was what I initially needed them for)

If they say no, or you need more look up medical supply places online. A few require a prescription, many others don't.

On a side note, requiring a prescription for syringes/needles is just stupid. There are plenty of legitimate reasons for them, AND  it limits access to clean needles for drugies. [rant] all the drugs that you inject cost WAY more than a needle... why the  #@()#$*#() not buy clean ones and make sure you don't get HIV/hep. b/ etc[/rant]


--------------------
If you try to lick your cat while tripping, and they bite you, are you even?

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OfflineSpongiform
Some Cow
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Registered: 08/22/07
Posts: 3,994
Loc: Greener Pastures
Last seen: 8 years, 6 months
Trusted Cultivator
Re: Liquid Culture Questions? [Re: alkylbenzene23]
    #9238672 - 11/13/08 04:54 PM (15 years, 4 months ago)

I'd buy them from KV Vet->
http://www.kvvet.com/KVVet/product_family.asp?family_id=211&gift=False&mscssid=D8F52CD7389B4BCA8B7F796E2998576E

http://www.kvvet.com/KVVet/product_family.asp?family_id=212&gift=False&mscssid=D8F52CD7389B4BCA8B7F796E2998576E

I found most of the tek's on the LC's had one flaw.  They assumed all the different karo/honeys had the same sugar content. 

If you read the shroomery Liquid Culture Bascis you'll see that it says :
Quote:


Liquid cultures are normally at a 4% dilute solution of various sugars and other nutrients in water.This would be 4 grams of sugars per 96 ml/cc water. (Water weighs 1 gram per ml/cc.)




The different types of corn syrups available at my local store had drastically different amounts of sugar (some had 2-3x as others).
So keep an eye on that.

As far as how much liquid culture you want, that's really up to you.. how much do YOU want to use? :-D

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Offlinealkylbenzene23
Indeed
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Registered: 08/16/08
Posts: 615
Last seen: 14 years, 4 months
Re: Liquid Culture Questions? [Re: alkylbenzene23]
    #9240208 - 11/13/08 08:03 PM (15 years, 4 months ago)

Honey is more or less constant in its sugar content.

also,

increasing the amount of water vs sugar helps prevent the formation of particulates during pressure cooking.


--------------------
If you try to lick your cat while tripping, and they bite you, are you even?

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Offlinedothedew69
Relearning
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Registered: 08/27/08
Posts: 624
Last seen: 3 years, 4 months
Re: Liquid Culture Questions? [Re: alkylbenzene23]
    #9240600 - 11/13/08 08:59 PM (15 years, 4 months ago)

Karo LC is by far the easiest. Spend the extra 50 cents and buy the Karo brand corn syrup in your baking aisle.

Search is a very powerful feature on this website just keep that in mind :smile:. These questions have probably been answered a bunch of times. Also for supplies check the vendors or Google search. So search search search. The basic information is easily obtained, just need to put forth a little effort. As you get more experienced with searching you will search for more complicated question and thus a learning experience will occur :wink:

Edited by dothedew69 (11/13/08 09:02 PM)

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OfflineShroomer Smurf
Slacker


Registered: 11/02/08
Posts: 176
Last seen: 11 years, 11 months
Re: Liquid Culture Questions? [Re: dothedew69]
    #9246025 - 11/14/08 07:57 PM (15 years, 4 months ago)

Quote:

dothedew69 said:
Karo LC is by far the easiest. Spend the extra 50 cents and buy the Karo brand corn syrup in your baking aisle.





That's what I plan on using. I'm using quart jars, how much water and Karo should I put in each jar? Also, when the LC is low, can I squirt the last into a new jar of water and Karo and keep it going? Is this safer than grain to grain? How many days does it take for the LC to be ready to use?


Thanks Again. :stoned:

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