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Offlinetehgod
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Registered: 10/18/08
Posts: 9
Last seen: 12 years, 11 months
First attempt.. how likely am I to have contamination?
    #9097377 - 10/18/08 07:05 PM (15 years, 5 months ago)

So me and a couple friends gathered all the supplies we needed and attempted to inoculate some jars the other day. We had several issues that caused us to cut corners on sterility. Im just curious whether you folks think they will all be contaminated or not.

Here are the mistakes we made that Im aware of:
We didnt have a pressure cooker, so we used the boiling method and boiled the jars for 90 minutes.
Once we realized how long it would take for them to cool, we had to clean out this black lunchboxish bag and put the jars in there. So we did that and had to put the bag (which remained zipped shut the whole time) in a less than tidy closet (we did spray down the area for what its worth, though Im not sure how effective that really was). Then when inoculating, I used a BIC lighter to sterilize the needle between jars, but did not heat until it glowed red.

And that's where we're at now.. a day later. Things definitely didn't go as well as we planned and now that Ive written all this out, it seems like a recipe for disaster. Does anybody think any of them will develop normally?

Edited by tehgod (10/18/08 07:07 PM)

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Offlineveda_sticks
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Re: First attempt.. how likely am I to have contamination? [Re: tehgod]
    #9097400 - 10/18/08 07:14 PM (15 years, 5 months ago)

You cant cut corners in this hobby. Follow the tek to a t and DONT CUT CORNERS.

Im not saying you have failed, you may get lucky.

You'll just have to wait and see.

www.mushroomvideos.com

watch all the pf tek videos.

You should be usiung a glovebox and definatly flaming till red hot.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinetehgod
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Re: First attempt.. how likely am I to have contamination? [Re: veda_sticks]
    #9097422 - 10/18/08 07:20 PM (15 years, 5 months ago)

I was really unsure about that part. I had seen one video series where the guy used a plastic tub to inoculate in and one where he just did it on the counter top. Better safe than sorry I suppose. We aren't expecting much, being our first attempt and all, so it won't be the worst thing in the world if we fail miserably. I will definitely try the glove box technique next time. I have about 3 ml of spore solution left to work with if all of these fail.

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Offlinetehgod
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Re: First attempt.. how likely am I to have contamination? [Re: veda_sticks]
    #9097436 - 10/18/08 07:23 PM (15 years, 5 months ago)

That's a really nice site by the way, thanks for the link.

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Offlineveda_sticks
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Re: First attempt.. how likely am I to have contamination? [Re: tehgod]
    #9097477 - 10/18/08 07:35 PM (15 years, 5 months ago)

If those turn out ok, take prints. If they go south, then use the rest of your solution to knock up a few more jars being more carefull with sterility and take prints.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Offlinegofudgeyourself
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Registered: 09/01/08
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Re: First attempt.. how likely am I to have contamination? [Re: veda_sticks]
    #9097517 - 10/18/08 07:49 PM (15 years, 5 months ago)

i didnt flame the needle for the first 5 jars i nocd and none of them contaminated....its a crapshoot. youll be lucky if it doesnt contam, just remember for next time.


--------------------
"believe it if you need it, or leave it if you dare."

"turn all the hate in the world, into a mocking bird. make it fly away" :peace:

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Offlinedothedew69
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Re: First attempt.. how likely am I to have contamination? [Re: gofudgeyourself]
    #9097549 - 10/18/08 07:57 PM (15 years, 5 months ago)

Well the more sterilization techniques the higher percentage of success rate it would seem. So inversely the less precautions taken, the greater the chance of contams.

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OfflineIcAnFeeLthErain
How ya like your Chang's Sauce?
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Re: First attempt.. how likely am I to have contamination? [Re: dothedew69]
    #9097586 - 10/18/08 08:08 PM (15 years, 5 months ago)

I think you have a 50/50 shot. 

Quote:

Once we realized how long it would take for them to cool, we had to clean out this black lunchboxish bag and put the jars in there.




Pardon me for being presumptive, but I would heavily discourage against cultivating in your parent's/guardian's house.  IME it didn't turn out very well.


--------------------
"We are here to awaken from the illusion of our separateness" -Thich Nhat Hanh

Take your sweet time, everything is a once in a life time experience.

Cakes?  No Thanks.

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Offlinetehgod
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Re: First attempt.. how likely am I to have contamination? [Re: IcAnFeeLthErain]
    #9097737 - 10/18/08 08:34 PM (15 years, 5 months ago)

Guess again.

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OfflineIcAnFeeLthErain
How ya like your Chang's Sauce?
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Registered: 05/24/08
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Re: First attempt.. how likely am I to have contamination? [Re: tehgod]
    #9098220 - 10/18/08 11:06 PM (15 years, 5 months ago)

Poor grandma.


--------------------
"We are here to awaken from the illusion of our separateness" -Thich Nhat Hanh

Take your sweet time, everything is a once in a life time experience.

Cakes?  No Thanks.

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Offlinetehgod
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Registered: 10/18/08
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Re: First attempt.. how likely am I to have contamination? [Re: IcAnFeeLthErain]
    #9099830 - 10/19/08 12:31 PM (15 years, 5 months ago)

You should either be friendly, helpful, or quiet.

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OfflineIcAnFeeLthErain
How ya like your Chang's Sauce?
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Registered: 05/24/08
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Re: First attempt.. how likely am I to have contamination? [Re: tehgod]
    #9099940 - 10/19/08 12:51 PM (15 years, 5 months ago)

Now your just being a little douchbag.

As you may recall I was being quite polite.  Remember when I said "pardon me for being presumptive...".  Well your response was obviously not sincere, but in jest, and perhaps provocative.  Let me refresh your memory...

Quote:

Pardon me for being presumptive, but I would heavily discourage against cultivating in your parent's/guardian's house.  IME it didn't turn out very well.

Quote:

Guess again.









Now that you've had a chance to remove your head from your anus you might be able to see that "Pardon me for being presumptive" was friendly.  And "I would heavily discourage...IME it didn't turn out very well" was helpful.  Now I think I'll be quite, good luck young buck.


--------------------
"We are here to awaken from the illusion of our separateness" -Thich Nhat Hanh

Take your sweet time, everything is a once in a life time experience.

Cakes?  No Thanks.

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Offlinej.guit.err
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Registered: 10/17/08
Posts: 213
Last seen: 14 years, 9 months
Re: First attempt.. how likely am I to have contamination? [Re: IcAnFeeLthErain]
    #9100141 - 10/19/08 01:55 PM (15 years, 5 months ago)

Quote:

IcAnFeeLthErain said:
Now that you've had a chance to remove your head from your anus you might be able to see that "Pardon me for being presumptive" was friendly.  And "I would heavily discourage...IME it didn't turn out very well" was helpful.  Now I think I'll be quite, good luck young buck.





:thumbup:


--------------------
"We can't trust the sword of a thousand truths to a newb!"
-Looking for prints, anything will do besides B Plus and EQ!
-PM me your offers!

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Offlinetehgod
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Registered: 10/18/08
Posts: 9
Last seen: 12 years, 11 months
Re: First attempt.. how likely am I to have contamination? [Re: IcAnFeeLthErain]
    #9116229 - 10/22/08 03:14 PM (15 years, 5 months ago)

Starting a sentence with "pardon me for being presumptive" does not make it a friendly or helpful one. All you did was make an assumption and then criticize me for the assumption you made. I am looking for criticism, but constructive criticism based on things I actually did do wrong.

All this aside, it does look like we have some mycelium growth. We're gonna give it a couple more days and then maybe take some pictures.

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OfflineNuzzle
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Registered: 09/29/08
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Re: First attempt.. how likely am I to have contamination? [Re: tehgod]
    #9116242 - 10/22/08 03:17 PM (15 years, 5 months ago)

I say use the leftover spore solution to make some LC.
Follow this tek and you should have no problems... And plenty of mycil to knock up all the jars you want! :smile:

http://www.shroomery.org/forums/showflat.php/Cat/0/Number/2321056/an/0/page/0


--------------------
:hi:**If you have any spare spore prints lying around, and your feeling 'giving' drop me a PM** :dancer:

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OfflinePOWAtrippinDiscord
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Registered: 10/22/08
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Re: First attempt.. how likely am I to have contamination? [Re: Nuzzle]
    #9116378 - 10/22/08 03:39 PM (15 years, 5 months ago)

Could not heating up the needle to the correct degree make the whole syringe go bad at this point?

The most important part of this story was left out but I am assuming you are attempting the PF tek like most people who are on their first time.  If this is true you might have a decent chance my buddy did an on the counter inoculation and was fine but he sterilized the needle as well.  Maybe a shade of 50/50 but I have seen people get 100% when I thought they would fail.  Its all just a crap shoot.


--------------------
Don't believe everything you think.  TRADE LIST

‹Sell Your Soul› You know this place is owned and operated by the Illuminati, right?
‹lsdwithme› i possibly just smoked a rat turd :facepalm:

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