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OfflineOyagoteneytokay
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Transfering mycelium to agar (PF Tek)
    #9056896 - 10/10/08 12:18 AM (15 years, 5 months ago)

Lets say I had full colonization in a jar (PF TEK) and I wanted to transfer some onto agar in a petri dish for reproduction.

What would be the best method to do this?

Also, would I be able to transfer it to rye grain straight from the pf tek cake?

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OfflineScavengerType
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Re: Transfering mycelium to agar (PF Tek) [Re: Oyagoteneytokay]
    #9057094 - 10/10/08 01:44 AM (15 years, 5 months ago)

assuming you've already got your agar and dish (unless I'm misunderstanding you) you simply transfer a small chunk in your Glovebox or in front of your flowhood. You could do the agar dish then do the grain jar then birth the cake.


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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
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OfflineScavengerType
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Re: Transfering mycelium to agar (PF Tek) [Re: ScavengerType]
    #9057097 - 10/10/08 01:45 AM (15 years, 5 months ago)

I know RR had a great segment of his videos on agar transfer.
http://www.mushroomvideos.com/


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"Have you ever seen what happens when a grenade goes off in a school? Do you really know what you’re doing when you order shock and awe? Are you prepared to kneel beside a dying soldier and tell him why he went to Iraq, or why he went to any war?"
"The things that are done in the name of the shareholder are, to me, as terrifying as the things that are done—dare I say it—in the name of God. Montesquieu said, "There have never been so many civil wars as in the Kingdom of God." And I begin to feel that’s true. The shareholder is the excuse for everything."
- Author and former M6/M5 agent John le Carré on Democracy Now.
Conquer's Club

Edited by ScavengerType (10/10/08 01:46 AM)

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OfflineGroomies
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Re: Transfering mycelium to agar (PF Tek) [Re: Oyagoteneytokay]
    #9057105 - 10/10/08 01:49 AM (15 years, 5 months ago)

in a clean glove box you could place one grain of myce you want onto a agar plate.


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:hotidea::hotidea::hotidea:

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Offlineveda_sticks
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Re: Transfering mycelium to agar (PF Tek) [Re: Groomies]
    #9057250 - 10/10/08 04:30 AM (15 years, 5 months ago)

Yes you can, in your glovebox, birth the cake and take a small peice the size of a grain of rice from the side of your cake and place in the middle of your agar petri dish. Do this quickly, you want to lift the lid of the petri dish for a minimum amount of time.

dont forget to rap parafilm around the edge of your petri dish to stop comtaminents entering from the side.

mushroomvideos.com has lots of usefull information that will get your started.

Buy the DVD to see everything


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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OfflineOyagoteneytokay
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Re: Transfering mycelium to agar (PF Tek) [Re: ScavengerType]
    #9060098 - 10/10/08 05:36 PM (15 years, 5 months ago)

I do not have the agar just yet. There's a health food store that has it right by my place though.

Do I just need to prep the agar itself or is there anything else I should be adding to it?

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OfflineDamion5050
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Re: Transfering mycelium to agar (PF Tek) [Re: Oyagoteneytokay]
    #9060226 - 10/10/08 06:10 PM (15 years, 5 months ago)


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