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Invisibleteknix
𓂀⟁𓅢𓍝𓅃𓊰𓉡 𓁼𓆗⨻
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Registered: 09/16/08
Posts: 11,953
My homeade self healing injection port.
    #9039478 - 10/06/08 09:37 PM (15 years, 5 months ago)

What I do is punch a hole in the lid where I want my injection port.

I take a piece of polyfil and twist it as tightly as I can , then put it into the hole.

I trim both side of the excess polyfil off with siscors.

Then I briefly use my lighter to (compact the polyfil and give it a more uniform surface.

I apply a liberal amount of silicone to either side of the polyfil, making sure the entire surface is covered.

Before injection I will suck a small amount of alchohol (70%) with a syringe and inject it into the polyfil, then continue through the bottom layer of the silicone and inject it.

I know u can just use the silicone alone and it would work, but I feel this will give me an added barrier against contamination while inserting and removing the needle.

I want to eventually try this without applying flame sterilazion, without a still air box. However at this point, Im still new to it all and will use a still air bx and flame sterilization.

Any thoughts of comments?

I appologize if this has already been posted, I just didnt see it.

© Tuesday, October 06, 2008


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

Edited by teknix (10/07/08 12:03 AM)

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Offlinefungiamongi2
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Re: My homeade self healing injection port. [Re: teknix]
    #9039526 - 10/06/08 09:47 PM (15 years, 5 months ago)

so you have polyfil covered in silicone?    seems like it would be prone to more contams b/c there is extra stuff to hold anything that contams, and when that gets wet, how is it gonna dry?    i could be way off too.


--------------------
gonna bring her a kiss, make those blues run

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InvisibleTheShroomJew23
The Chemist
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Registered: 11/29/07
Posts: 1,611
Loc: Temple
Re: My homemade self healing injection port. [Re: fungiamongi2]
    #9039539 - 10/06/08 09:50 PM (15 years, 5 months ago)

When you PC polyfill it melts  into a impenetrable puck.


--------------------


You will be missed

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OfflineJuke Adro
I love peach fluff
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Registered: 04/05/08
Posts: 6,957
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Re: My homemade self healing injection port. [Re: TheShroomJew23]
    #9039562 - 10/06/08 09:55 PM (15 years, 5 months ago)

Quote:

TheShroomJew23 said:
When you PC polyfill it melts  into a impenetrable puck.


Rubbish it does not melt at all it can with stand 20psi


--------------------
Someone said:  im actually not using ms, im using prints.
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OfflineJeets
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Registered: 07/06/08
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Re: My homemade self healing injection port. [Re: Juke Adro]
    #9039573 - 10/06/08 09:57 PM (15 years, 5 months ago)

Quote:

Juke Adro said:
Quote:

TheShroomJew23 said:
When you PC polyfill it melts  into a impenetrable puck.


Rubbish it does not melt at all it can with stand 20psi





Yes agreed it can withstand heat well. The only thing is you may get a little condensation in it, that's all

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Invisibleteknix
𓂀⟁𓅢𓍝𓅃𓊰𓉡 𓁼𓆗⨻
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Registered: 09/16/08
Posts: 11,953
Re: My homemade self healing injection port. [Re: Jeets]
    #9039595 - 10/06/08 10:01 PM (15 years, 5 months ago)

The thing that worry's me the most is if I dont get all the alchohol out in the polyfil and squirt some into into my LC. I dont know if a minute amount could spell catastrophe?!? If so, I will take extra precaution and inject a little more  than what I sucked up into the polyfil layer.

Edited by teknix (10/07/08 03:29 PM)

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Offlineneybis
Master of the Custodial Arts


Registered: 08/11/08
Posts: 190
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Re: My homemade self healing injection port. [Re: Jeets]
    #9039599 - 10/06/08 10:02 PM (15 years, 5 months ago)

im really curious if this would work or not because i was planning on starting LC's soon and need the self healing ports. Any old hands have any input as to whether or not this could possibly lead to contams due to the fact that it might hold IN the moisture??


--------------------
"Why is there so much controversy about drug testing? I know plenty of guys who would be willing to test any drug they could come up with."

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Invisibleteknix
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Posts: 11,953
Re: My homeade self healing injection port. [Re: teknix]
    #9039633 - 10/06/08 10:11 PM (15 years, 5 months ago)

Also instead of adding a filter for gas exchange, I plan on injecting sterile air directly into the jar once every so often.

To do this I will suck a small amount of alcohol into the syringe. I will then light a candle, stick my needle into the flame to sterilize it. I will raise the needle a few inches above the flame and suck in air. I will then inject the alcohol into the polyfil layer, then proceed throught the bottom layer of silicone and inject the air.

I dont know if a positive pressure environment would be good or bad. I plan to test this as soon as my spores/myc arrive.

Edited by teknix (10/07/08 03:27 PM)

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InvisibleTheShroomJew23
The Chemist
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Posts: 1,611
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Re: My homeade self healing injection port. [Re: teknix]
    #9039646 - 10/06/08 10:16 PM (15 years, 5 months ago)

My polyfill always melts, thats why I only use tyvek and micropore tape. I love tyvek so much anyways.


--------------------


You will be missed

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Invisibleteknix
𓂀⟁𓅢𓍝𓅃𓊰𓉡 𓁼𓆗⨻
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Posts: 11,953
Re: My homeade self healing injection port. [Re: TheShroomJew23]
    #9039668 - 10/06/08 10:20 PM (15 years, 5 months ago)

Quote:

TheShroomJew23 said:
My polyfill always melts, thats why I only use tyvek and micropore tape. I love tyvek so much anyways.




If this is the case, I will try it with a fresh filter from a cigarette. I hope they both dont melt, otherwise its back to the drawing board :P.


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

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OfflineDamion5050
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Registered: 05/01/08
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Trusted Cultivator
Re: My homeade self healing injection port. [Re: teknix]
    #9039694 - 10/06/08 10:25 PM (15 years, 5 months ago)

if they keep melting invest is some synthetic filter discs from mycosupply

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InvisibleTheShroomJew23
The Chemist
Male

Registered: 11/29/07
Posts: 1,611
Loc: Temple
Re: My homeade self healing injection port. [Re: Damion5050]
    #9039709 - 10/06/08 10:28 PM (15 years, 5 months ago)

Don't use cig filters, they rot. They are meant to biodegrade now and they will just turn to mush in not long.


--------------------


You will be missed

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OfflineJuke Adro
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Re: My homeade self healing injection port. [Re: TheShroomJew23]
    #9039737 - 10/06/08 10:31 PM (15 years, 5 months ago)

Ear plugs work


--------------------
Someone said:  im actually not using ms, im using prints.
Trade List

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Invisibleteknix
𓂀⟁𓅢𓍝𓅃𓊰𓉡 𓁼𓆗⨻
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Registered: 09/16/08
Posts: 11,953
Re: My homeade self healing injection port. [Re: Juke Adro]
    #9039779 - 10/06/08 10:40 PM (15 years, 5 months ago)

Quote:

Juke Adro said:
Ear plugs work




Good call there. I actually have some of these laying around here somewhere.


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

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Offlinehowsitgoingtoend
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Re: My homemade self healing injection port. [Re: teknix]
    #9039898 - 10/06/08 11:07 PM (15 years, 5 months ago)

I've lived in some shit holes and jar contamination was never a huge problem. On Average I'd get 2-3 jars out of 8 that went south but that wasn't bad considering the environment and my lack of any fancy equipment. Save your money and stick with the vermiculite layer and four small holes with tape over them.

I'd worry more about the sterile syringe making process.

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Offlinehaze666
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Re: My homemade self healing injection port. [Re: howsitgoingtoend]
    #9039916 - 10/06/08 11:10 PM (15 years, 5 months ago)

What is the point of pf in the middle of the silicone?  I heard of a squirrel having a 100% success rate with silicone and no poly.

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Invisibletripchip
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Re: My homemade self healing injection port. [Re: haze666]
    #9039955 - 10/06/08 11:20 PM (15 years, 5 months ago)

you got the wrong polyfill if it melts.


--------------------
Psilocybe Cubensis

Psilocybe comes from the Greek root "psilos" meaning bald head and Cubensis because it was first recognized as a new species from specimens collected in Cuba.

                                    :mushroom2:CHIP:mushroom2:

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Invisibleteknix
𓂀⟁𓅢𓍝𓅃𓊰𓉡 𓁼𓆗⨻
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Posts: 11,953
Re: My homemade self healing injection port. [Re: haze666]
    #9039987 - 10/06/08 11:28 PM (15 years, 5 months ago)

Quote:

haze666 said:
What is the point of pf in the middle of the silicone?  I heard of a squirrel having a 100% success rate with silicone and no poly.



Actually the intention is to minimize the sterile procedures when injecting LC, although it may be able to be applied to other applications as well.


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

Edited by teknix (10/06/08 11:36 PM)

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Offlineveda_sticks
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Re: My homemade self healing injection port. [Re: teknix]
    #9040635 - 10/07/08 03:58 AM (15 years, 5 months ago)

You will still have to flame sterilise before inoculating each jar. Just because you have alcohol in the polyfill between your silicon is not going to sterilise you needle.

We flame the needle red hot not to kill anything that has landed on the needle, but anything that may have worked its way inside the needle.

Always flame your needle red hot. You can make your own alcohol lamp with a 1/2 pint canning jar. Punch a small hole in the lid and feed a peice of string through the lid so the string sits in the alcohol and you have a little bit through the top. Light.


--------------------

PF TEK - writeup by EvilMushroom666
Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD
Cakes can and will pin! - So you think cakes suck for pins. Your wrong
Franks Simple Coir/Verm Tek
Franks Proper Pasturisation Tek
Franks Spawning To Bulk - Monotub
Professor Pinheads RTV Injection Port Tek
Foo Mans No Soak WBS Prep Tek

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Invisibleteknix
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Registered: 09/16/08
Posts: 11,953
Re: My homemade self healing injection port. [Re: veda_sticks]
    #9040669 - 10/07/08 04:31 AM (15 years, 5 months ago)

Good morning veda, see you've been busy ^^

I realize that alcohol sanitizes. This is more for an added layer of protection, as LC is highly contaminable.


--------------------
.6th and 7th sense theory
.Now is forever. .ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞTheﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞUnseenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ is seenﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ by the blindﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ eye.ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ.When the inevitable time comes, go with your head held high,without regret or remorse, in your subconscious mind.
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ
ﱞﱞﱞﱞﱞﱞﱞﱞﱞﱞ

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