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Bababooey
Stranger
Registered: 08/29/08
Posts: 8
Last seen: 15 years, 5 months
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What am I doing wrong?
#8995232 - 09/27/08 06:45 PM (15 years, 6 months ago) |
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Heres something I cant understand. I started growing YEARS ago and have had countless grows for experience. I recently started again after about 2 years off. What I cant figure out is the amount of contamination Im getting. Ive only got about a 20% success rate. Out of the 5 dozen jars (all are PF tek) Ive mixed up, I have a total of 10 cakes. I have NEVER had a problem with green mold, yet my last batch is totally contaminated. Other times they just dont grow but have a horrible smell. I almost want to say Ive got a bad batch of spores. Looking at the last batch of jars just now, all the green mold is in the injection sites where the water was squirted. Im not going to call out the spore supplier because they are making it right. Im more pissed off at the time wasted prepping the jars just to have them contaminate.
Back when I was young and stupid I had a 80+ dozen jar grow that went on for about a year. I would only pick at most 5 or 6 contaminated jars out of the whole batch! This was also doing all the prep stoned out of my mind!
Like I said, I cant figure out where Im going wrong. When the jars are finished, they are washed, rings wiped out, new tape, and pressure cooked before they get any mix put in. After theyre full, I wipe rim to get any excess off. Theyre pressure cooked for 45min. Before I inject, I spray my room down with lysol, wash hands, and flame the needle until its red hot. Is it me or the syringes? Any ideas?
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HippieChick
Chicks can do it too!
Registered: 02/20/05
Posts: 5,958
Loc: Midwest
Last seen: 3 years, 2 months
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Re: What am I doing wrong? [Re: Bababooey]
#8995263 - 09/27/08 06:50 PM (15 years, 6 months ago) |
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Sounds to me that you may have gotten a bad syringe . It happens . Contact the supplier and I'm sure they'll take care of it .
When you get another spore syringe , I'd recommend making a LC from it . Test that out on a couple jars and if all goes well , use your LC . It will greatly decrease your colonization times on jars . I've inoculated pf cakes with LC and had fruits in 16 days from inoculation
Peace,Love and Happiness HC
-------------------- Peace,Love and Happiness HC Freedoms just another word for nothing left to lose.............. I LUV My Greenhouse http://www.shroomery.org/forums/showflat.php/Number/5545848#5545848 My First Pans http://www.shroomery.org/forums/showflat.php/Number/6212058#6212058
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fishfarmin
Predator Hunter
Registered: 09/04/08
Posts: 196
Last seen: 14 years, 11 months
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Re: What am I doing wrong? [Re: HippieChick]
#8995282 - 09/27/08 06:52 PM (15 years, 6 months ago) |
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i agree it is most likely the syringe.:(
-------------------- "Government exist to protect us from each other.Where government has gone beyond its limits is in deciding to protect us from ourselves." Ronald Reagan "It is error alone which needs the support of government.Truth can stand by itself."Thomas Jefferson "It has been my experience that folks who have no vices have very few virtues."Abraham Lincoln
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phaseflux
Stranger than most
Registered: 07/16/08
Posts: 702
Last seen: 10 years, 5 months
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Re: What am I doing wrong? [Re: Bababooey]
#8995285 - 09/27/08 06:53 PM (15 years, 6 months ago) |
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Are you making the syringes yourself from prints or just buying pre-made syringes? Sounds like it could indeed be a spore problem to me, unless your not sterilizing your jars well enough. What are you using? PF Tek?
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Juke Adro
I love peach fluff
Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 4 years, 11 months
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Re: What am I doing wrong? [Re: Bababooey]
#8995289 - 09/27/08 06:54 PM (15 years, 6 months ago) |
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I think the problem is that you never moved on to bulk and pasteurisation, usually you try pf then that teaches you how mushrooms grow and what to look for and so on, then you move on to the next step which is actually less time consuming, faster results and allot more fruit.
If its only at the inoculation points I would either believe its the syringe or you technique and un sterile procedures also It could be the fact that your only doing the jars for 45 minutes that's really not long enough
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ray40cal
omnitrippint
Registered: 05/05/08
Posts: 1,308
Loc: midwest side
Last seen: 15 years, 4 months
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Re: What am I doing wrong? [Re: Juke Adro]
#8995325 - 09/27/08 07:00 PM (15 years, 6 months ago) |
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It's gotta be the syringe solution. Even if you only pc'd 45 minutes that's not going to give you an 80% contam rate.... If you flame the needle before every jar that's good too.
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iMtRiPpIn
Lookin' fer Luv
Registered: 06/04/08
Posts: 530
Last seen: 11 years, 5 months
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Re: What am I doing wrong? [Re: ray40cal]
#8995493 - 09/27/08 07:31 PM (15 years, 6 months ago) |
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Could it be that you just weren't stoned out of your mind this time when you prepped the jars? maybe that's the prob.
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Bababooey
Stranger
Registered: 08/29/08
Posts: 8
Last seen: 15 years, 5 months
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Re: What am I doing wrong? [Re: iMtRiPpIn]
#8995746 - 09/27/08 08:28 PM (15 years, 6 months ago) |
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Thanks for the replies. Yes, this is PF tek and the syringes were ordered. Like I said, I never had a contam problem before. I'm OCD with cleaning things so the house is spotless. Mostly I'm just frustrated after having no problems before and all of a sudden everything is wrong.
I'm experimenting with LC and bulk substrates. I did 3 LC jars and had 2 come out fine and the other grew black. My first two attempts at WBS had massive contam problems until I found out they were too wet. I did one jar and dried the seed out then PC'ed it and it's coming along nicely. If this works I'll be doing bulk 100%. Believe me, after having mixed 80 dozen jars in a day countless times, I've paid my dues with PF!
Considering my "state" right now, maybe I should mix some jars....
Edited by Bababooey (09/27/08 08:30 PM)
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Juke Adro
I love peach fluff
Registered: 04/05/08
Posts: 6,957
Loc: Inside your head
Last seen: 4 years, 11 months
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Re: What am I doing wrong? [Re: Bababooey]
#8995774 - 09/27/08 08:36 PM (15 years, 6 months ago) |
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WBS is a liitle bitch, I prefer Rye grain its cheaper that WBS down here also, just make sure you soak the WBS for 24 hours and up to *36 hours *if you use hot water to stop them from sprouting, simmer them for ten mins with some coffee grounds for some added flavour or use it in the soak.
The best of luck with your bulk trials
-------------------- Someone said: im actually not using ms, im using prints. Trade List
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 14,191
Loc: UK
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Re: What am I doing wrong? [Re: Juke Adro]
#8997215 - 09/28/08 08:18 AM (15 years, 5 months ago) |
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use a glovebox, while some have great success with cleaning a small room and inoculate there, others it just doesnt work.
make a simple glovebox, you will need one if you want to do g2g or agar.
-------------------- PF TEK - writeup by EvilMushroom666 Lets Grow Mushrooms - RogerRabbit & RoadKills website with sample videos plus the full PF TEK video series. Alot of great information - BUY THE DVD Cakes can and will pin! - So you think cakes suck for pins. Your wrong Franks Simple Coir/Verm Tek Franks Proper Pasturisation Tek Franks Spawning To Bulk - Monotub Professor Pinheads RTV Injection Port Tek Foo Mans No Soak WBS Prep Tek
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