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Offlinenenl
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Replenishing LC?
    #8994593 - 09/27/08 04:45 PM (15 years, 5 months ago)

This might be a common practice, but after a quick search I didn't find any info. Anyway, I have a good LC going and what I plan to do is replenish what I take out by making more karo solution to inject after I take some out. Would be something in the range of 100cc exchange.

1. Comments on that idea?

2. If contam is introduced when injecting more solution do you think the contam would affect the LC? Based on a "good" amount of myc in the LC and the injected solution would be PC'd, alcohol wipe and flame needle, etc so if any got it it should be minimal - would the myc kill any contam that snuck in?

Thanks all...


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InvisibleSFsorrow
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Re: Replenishing LC? [Re: nenl]
    #8994624 - 09/27/08 04:50 PM (15 years, 5 months ago)

There is to great of a risk at introducing more contamination, which would ruin the LC. Just make multiple jars at a time and stagger their use.

A contaminant can take hold in the new solution you injected to it and live side by side the dormant myc. Once you inoculate it to your growing medium, you are injecting both myc and the contaminant, not good.

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OfflineBrandNewbie
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Re: Replenishing LC? [Re: nenl]
    #8994629 - 09/27/08 04:50 PM (15 years, 5 months ago)

Why don't you just use some good LC to start another jar? :shrug:


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Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.

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InvisibleSFsorrow
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Re: Replenishing LC? [Re: BrandNewbie]
    #8994657 - 09/27/08 04:53 PM (15 years, 5 months ago)

Quote:

BrandNewbie said:
Why don't you just use some good LC to start another jar? :shrug:




Be careful with this. The myc will degrade in quality with each new generation that you start. It's best to have a master culture and only use that to start a second generation culture.

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InvisibleR10tGuNn3r

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Re: Replenishing LC? [Re: SFsorrow]
    #8994661 - 09/27/08 04:53 PM (15 years, 5 months ago)

or instead... use that 100cc and introduce it into a new LC.. that way you dont risk contaminating your master lc... and you get more lc in a new jar... just be sure to check it for contams before you use the rest of the master and you should be golden..

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OfflineThe Cake Guy
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Re: Replenishing LC? [Re: R10tGuNn3r]
    #8994673 - 09/27/08 04:57 PM (15 years, 5 months ago)

Quote:

Be careful with this. The myc will degrade in quality with each new generation that you start. It's best to have a master culture and only use that to start a second generation culture.




So if you knock up an LC with an LC then it the mycelium will get weaker every time??


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Offlinenenl
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Re: Replenishing LC? [Re: The Cake Guy]
    #8994712 - 09/27/08 05:05 PM (15 years, 5 months ago)

Thanks all for the input. I'll try this with one jar and simply make new jars to replace others.


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OfflineBrandNewbie
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Re: Replenishing LC? [Re: The Cake Guy]
    #8994884 - 09/27/08 05:43 PM (15 years, 5 months ago)

Yes. If you use each "new" jar of LC to inoculate another.

A "master" is a jar that you use to inoculate others with. That way you are always using second generation, as opposed to very rapid degradation as the generations increase exponentially. :thumbup:


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Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.

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Offlinenenl
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Re: Replenishing LC? [Re: BrandNewbie]
    #8994956 - 09/27/08 05:55 PM (15 years, 5 months ago)

Quote:

BrandNewbie said:

A "master" is a jar that you use to inoculate others with. That way you are always using second generation, as opposed to very rapid degradation as the generations increase exponentially. :thumbup:




What's the best way to maintain a master jar - that's why I had my original question? I have some good stuff in there that I'd live to keep around.


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InvisibleR10tGuNn3r

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Re: Replenishing LC? [Re: nenl]
    #8994998 - 09/27/08 06:02 PM (15 years, 5 months ago)

Quote:

nenl said:
Quote:

BrandNewbie said:

A "master" is a jar that you use to inoculate others with. That way you are always using second generation, as opposed to very rapid degradation as the generations increase exponentially. :thumbup:




What's the best way to maintain a master jar - that's why I had my original question? I have some good stuff in there that I'd live to keep around.



the best way is to use a master to inoculate second generation jars... it doesnt take very much of a master to inoculate a second generation jar.. so always use a second generation to inoculate your substrate.. a master LC should last a while if you are only using it to make a second generation LC when you run out of it.. make sense? also.. making large amount of master culture like a pint.. should give you all you need to keep it throughout its lifespan..

if you run out of the master... take one of the mushrooms that you get from that culture and make a new culture off of that... its a clone so it should have pretty much the same properties..

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Invisiblegeorge castanzaM
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Re: Replenishing LC? [Re: The Cake Guy]
    #8995028 - 09/27/08 06:06 PM (15 years, 5 months ago)

Quote:

The Cake Guy said:
Quote:

Be careful with this. The myc will degrade in quality with each new generation that you start. It's best to have a master culture and only use that to start a second generation culture.




So if you knock up an LC with an LC then it the mycelium will get weaker every time??




You should always go back to spores every third generation or so.  This should keep your strain from degrading.


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OfflineBrandNewbie
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Re: Replenishing LC? [Re: nenl]
    #8995039 - 09/27/08 06:10 PM (15 years, 5 months ago)

While R10tGuNn3r is absolutely correct, I'm going to answer the possible other meaning of your question, just in case.

Store it on a room temperature shelf. It'll be fine. Refrigerate if you not using it very quickly. :thumbup:


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Question: Why do women wear make-up and perfume? 
Answer: Because they're ugly and they stink.

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InvisibleCultivate

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Re: Replenishing LC? *DELETED* *DELETED* [Re: george castanza]
    #8995050 - 09/27/08 06:12 PM (15 years, 5 months ago)

Post deleted by Cultivate

Reason for deletion: .


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Invisibleseven
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Re: Replenishing LC? [Re: Cultivate]
    #8995128 - 09/27/08 06:28 PM (15 years, 5 months ago)

someone correct me if im wrong but using the word generation when refering to mycelium transfer is incorrect like expanding via g2g: the mycelium is still the same mycelium or generation. generation is from spore to fruit to spore. are we confusing cell division with generations?


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Invisiblegeorge castanzaM
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Re: Replenishing LC? [Re: Cultivate]
    #8995152 - 09/27/08 06:32 PM (15 years, 5 months ago)

Quote:

psillycake said:
Just curious........what are signs that a strain has "degraded"?




Less fruit, cracked caps, no fruit...  Strain not as good as it was (degraded)


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Invisibleseven
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Re: Replenishing LC? [Re: george castanza]
    #8995180 - 09/27/08 06:36 PM (15 years, 5 months ago)

Quote:

george castanza said:
Quote:

psillycake said:
Just curious........what are signs that a strain has "degraded"?




Less fruit, cracked caps, no fruit...  Strain not as good as it was (degraded)


wouldnt a sign be slow colonization? and cracked caps can point to low humidity right?


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grind

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InvisibleR10tGuNn3r

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Re: Replenishing LC? [Re: seven]
    #8995219 - 09/27/08 06:43 PM (15 years, 5 months ago)

Quote:

seven said:
someone correct me if im wrong but using the word generation when refering to mycelium transfer is incorrect like expanding via g2g: the mycelium is still the same mycelium or generation. generation is from spore to fruit to spore. are we confusing cell division with generations?



i was refering to generations of LC... its all the same mycelium from a single spore...

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Invisiblegeorge castanzaM
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Re: Replenishing LC? [Re: seven]
    #8995277 - 09/27/08 06:51 PM (15 years, 5 months ago)

Quote:

seven said:
Quote:

george castanza said:
Quote:

psillycake said:
Just curious........what are signs that a strain has "degraded"?




Less fruit, cracked caps, no fruit...  Strain not as good as it was (degraded)


wouldnt a sign be slow colonization? and cracked caps can point to low humidity right?




Not always, I had a strain that just always grew cracked caps.  About 95% of all the fruit was cracked caps, I always tried to print the non-cracked ones When a strain degrades it will not nessasarily be slow to colonize, it just will fruit poorly.  There are a lot of people around here that know a lot more about it, but I haven't seen them posting in the cult forum of late.  RR prolly knows more than I.  Any thought RR?


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Invisibleseven
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Re: Replenishing LC? [Re: george castanza]
    #8995308 - 09/27/08 06:57 PM (15 years, 5 months ago)

i was refering to the use of the word generation in lc or g2g. its not a good term because a second generation means a life cycle is started over ie: the grow completed and those spores are used for the second generation. when using a master lc to make a new lc that isnt a new generation: its only expanding the existing generation. T1 T2 transfer 1 transfer 2? i think im correct.


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Offlinefishfarmin
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Re: Replenishing LC? [Re: george castanza]
    #8995322 - 09/27/08 06:59 PM (15 years, 5 months ago)

:strokebeard:keep the info comin',im back into it after quite a few years away and will absorb as much as possible on the subject of lc's.im tired of using just petris and spore syringes everytime.:tripping2:


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