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 alkylbenzene23
Indeed
Registered: 08/16/08
Posts: 615
Last seen: 14 years, 4 months
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 Why so many spores? 
#8826413 - 08/25/08 06:24 PM (15 years, 7 months ago) |
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I notice that all the guides say use ~1-1.5ml/cc of solution per jar. So, this approximate hundreds if not thousands of spores per jar. Why so many? Shouldnt 4 or 5 spores per jar be sufficient(as small a squirt you can manage)?
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If you try to lick your cat while tripping, and they bite you, are you even?
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NobodyImportant
Stranger
Registered: 05/04/08
Posts: 1,016
Loc: Jawjuh.
Last seen: 11 years, 8 months
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4 or 5 might work but it could take a million times longer
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Psuper
Psilocybin
Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 7 months
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Dilute your syringes heavily if using this much. ~Pixie~
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Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk
Edited by Psuper (08/25/08 07:01 PM)
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 Alabama Slim
Chinese river witch
Registered: 06/29/08
Posts: 7,439
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Re: Why so many spores? *DELETED* [Re: Psuper]
#8826618 - 08/25/08 06:53 PM (15 years, 7 months ago) |
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Post deleted by Alabama SlimReason for deletion: delete
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alkylbenzene23
Indeed
Registered: 08/16/08
Posts: 615
Last seen: 14 years, 4 months
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Ok, so the guides are all wrong with putting so much in. That is what I thought. Since this stuff reproduces exponentially so long as food/area needs are met, and are needs are not met if you inject so many spores.
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If you try to lick your cat while tripping, and they bite you, are you even?
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RasJeph
RC Hoarder
Registered: 06/23/08
Posts: 3,598
Last seen: 8 months, 10 days
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I dunno it seems like it would take forever.
Try it and let us know.
Edited by RasJeph (08/28/08 05:54 AM)
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Psuper
Psilocybin
Registered: 02/12/08
Posts: 2,874
Loc: PNW
Last seen: 3 years, 7 months
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Re: Why so many spores? [Re: RasJeph]
#8826830 - 08/25/08 07:26 PM (15 years, 7 months ago) |
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Thought I would post something from RR on this subject here:
"Kinda depends on what you mean by inoculation. If you mean spores, then yes. Too many spores results in generally poor performance even though the jar might 'colonize' sooner, if you define colonization as being all white. Needless time and energy are expended in the process of anastomosis as the strains combine. Since some strains are not capable of diaryotic pairings, they fruit as separate zones on the substrate. This is why sometimes with multispore inoculation you get odd looking fruits that don't match the majority of the rest of the flushes, or second and third flushes look totally different from first flush."
-RR
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Clinical Management of High Dose Psilocybin Sessions:
https://www.youtube.com/watch?v=FbHOTIqjZLk
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gluvbox
mycophagist
Registered: 07/15/08
Posts: 145
Last seen: 14 years, 10 months
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Re: Why so many spores? [Re: Psuper]
#8827342 - 08/25/08 08:41 PM (15 years, 7 months ago) |
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With my most recent jars, I did exactly what you suggested and did a minimal amount of solution in each hole. I got through 10 jars with 7.5ccs of solution. I got germination within 4 days in an average of 3.4 points/jar(max=4, min=2).
By day 7, each germination has only grown to the size of a nickel. Only one germination point (out of 34) has shown bright white rhyzomorphic growth. The rest are still light gray fluff that hugs the BRF chunks.
It might be the case that more solution = fast colonization up to a point.
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Please contribute to the mycology wiki:
mycology.wikia.com
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alkylbenzene23
Indeed
Registered: 08/16/08
Posts: 615
Last seen: 14 years, 4 months
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Re: Why so many spores? [Re: gluvbox]
#8829320 - 08/26/08 06:10 AM (15 years, 7 months ago) |
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Two things.
How can you tell when they germinate?
And whats this "points"?
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If you try to lick your cat while tripping, and they bite you, are you even?
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Gojira45
Armchair Explorer
Registered: 08/04/08
Posts: 364
Loc: Travelling Without Moving
Last seen: 2 years, 3 months
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Quote:
alkylbenzene23 said:
I notice that all the guides say use ~1-1.5ml/cc of solution per jar. So, this approximate hundreds if not thousands of spores per jar. Why so many? Shouldnt 4 or 5 spores per jar be sufficient(as small a squirt you can manage)?
What you are assuming is that one of these 4 - 5 spores will find the ideal conditions to grow in. You obviously increase your chances when you have 100's - 1000's of spores (remember they are microscopic, so within a jar there is plenty of places for individual spores to grow). Using the same logic, too many spores (so called 'dark syringes') crowd out individual spores, decreasing the overall number of spores that might find their 'ideal' environment.
I dunno - spore syringes are 10 - 15 USD, and easily do 6 - 8 qt jars of grain, which you can then use G2G to extend. Do you really need to save spores that much?? There is a reason that each fruit produces so many spores....
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"The theft of the seals and the possibility of a Planet X really point to everything we are doing as humans.
It was all set up. Were doing the same thing Hitler did with artifacts and knowledge about hollow earth.
Everthing points to hollow earth and that ultimitaly points to planet X."
 
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: Why so many spores? [Re: Gojira45]
#8829533 - 08/26/08 08:09 AM (15 years, 7 months ago) |
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With fresh spores, only about 1 in 100 will germinate, perhaps less. By six months, only about 1 in 1,000 will germinate, and it goes downhill from there. However, we have long known that the best spore syringes are clear to the naked eye. You'll still be injecting a few thousand spores into each inoculation point. Just avoid dark syringes, and know they cause far more problems.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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gluvbox
mycophagist
Registered: 07/15/08
Posts: 145
Last seen: 14 years, 10 months
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Re: Why so many spores? [Re: RogerRabbit]
#8831477 - 08/26/08 04:34 PM (15 years, 7 months ago) |
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Hmmm... every syringe I've ever used was relatively dark (but of course it wasn't 100% opaque).
Say RR, to get a notion of what you think is the best spore to water ratio, how many ccs of solution would you make from a print that is the size of a 50 cent coin?
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Please contribute to the mycology wiki:
mycology.wikia.com
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Maverick
Lover of Earwigs!
Registered: 12/18/05
Posts: 13,443
Loc: Valleys of Willamette 
Last seen: 2 days, 37 minutes
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Re: Why so many spores? [Re: RogerRabbit]
#8831623 - 08/26/08 05:09 PM (15 years, 7 months ago) |
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Quote:
RogerRabbit said:
With fresh spores, only about 1 in 100 will germinate, perhaps less. By six months, only about 1 in 1,000 will germinate, and it goes downhill from there. However, we have long known that the best spore syringes are clear to the naked eye. You'll still be injecting a few thousand spores into each inoculation point. Just avoid dark syringes, and know they cause far more problems.
RR
By darker, you mean overloaded w/ spores that it's cloudy and opaque, or do you mean you can at least see some floating particulates (spores) in the water? I'm going to be making some syringes soon (hopefully) so it'd be nice to know how many spores i should suck back up into the syringe. 
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Gojira45
Armchair Explorer
Registered: 08/04/08
Posts: 364
Loc: Travelling Without Moving
Last seen: 2 years, 3 months
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Re: Why so many spores? [Re: Maverick]
#8832524 - 08/26/08 08:21 PM (15 years, 7 months ago) |
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Easy - just count out 2000 spores per CC. 2500 is too many  I joke, I joke.
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"The theft of the seals and the possibility of a Planet X really point to everything we are doing as humans.
It was all set up. Were doing the same thing Hitler did with artifacts and knowledge about hollow earth.
Everthing points to hollow earth and that ultimitaly points to planet X."
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seven
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Registered: 06/09/08
Posts: 1,478
Loc: north carolina
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Re: Why so many spores? [Re: Gojira45]
#8832816 - 08/26/08 09:08 PM (15 years, 7 months ago) |
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lets say a print isnt onehundred percent clean. the more spores from the print you put into the syringe, the more of a chance of takeing contams with you. but lets pray our prints are clean. :holds a little sayaunce to the tiki spore god: ::does a little tiki spore dance:: thank you! thank you!
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grind
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