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RonPaulVerm
Piss Christ
Registered: 07/21/08
Posts: 1,072
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Sterilze Needle..........
#8680390 - 07/25/08 01:18 PM (15 years, 7 months ago) |
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i am using the inoculation method of an alcohol dipped gauze pad held around the needle, and pressed against the jar as i inject the needle through the aluminum foil. Is it okay to sterilize the needle with alcohol and not a flame or something? And has this method of inoculation been proven good because it looked to be a good one
-------------------- Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech, Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have Primitive Skills Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms ...I DO advocate learning about Law Enforcement Against Prohibition
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robanero
β Lib
Registered: 09/05/06
Posts: 874
Loc: Somewhere in Tx
Last seen: 5 years, 9 months
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Re: Sterilze Needle.......... [Re: RonPaulVerm]
#8680430 - 07/25/08 01:33 PM (15 years, 7 months ago) |
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1st Welcome to The Shroomery.
2nd I only flame once between each cake and always wipe with 70% Iso after every hole.
3rd Take the foil off before you noc up. And if you have the foil under the lid, don't do that. That foil is supposed to be put over the lid to keep water from getting into the jar.
Happy growing.
-------------------- 310 open cap Hawaiians grown a 1 pint WBSF cake. SPAWN RATIO CALCULATOR My Little Hawaiians Spawn Bag Tek
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 29 days
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Re: Sterilze Needle.......... [Re: robanero]
#8680440 - 07/25/08 01:35 PM (15 years, 7 months ago) |
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Alcohol pads do nothing for the inside of the needle. Flame sterilize until red hot. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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robanero
β Lib
Registered: 09/05/06
Posts: 874
Loc: Somewhere in Tx
Last seen: 5 years, 9 months
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Re: Sterilize Needle.......... [Re: RogerRabbit]
#8680996 - 07/25/08 04:28 PM (15 years, 7 months ago) |
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Quote:
RogerRabbit said: Alcohol pads do nothing for the inside of the needle. Flame sterilize until red hot. RR
RR In no way am I suggesting he not flame sterilize the needle. Please don't get me wrong. This is assuming that the jars are PF jars. The needle should most definitely be flamed. I just do it between jars because whatever contam that may come from holes 1,2,3, or 4 is already in the jar. I flame after my 5th inoc of the jar so that there is no cross contamination.
I apologize for any confusion on my part.
-------------------- 310 open cap Hawaiians grown a 1 pint WBSF cake. SPAWN RATIO CALCULATOR My Little Hawaiians Spawn Bag Tek
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RonPaulVerm
Piss Christ
Registered: 07/21/08
Posts: 1,072
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Re: Sterilze Needle.......... [Re: RogerRabbit]
#8688158 - 07/27/08 12:27 PM (15 years, 7 months ago) |
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Can i use a cigarette lighter flame. in your movies i believe you said NOT to? what are the dangers?
-------------------- Spawn I Have: Blewitt, Reishi, Maitake, Chicken of the Woods, Parasol, Shaggy Mane, King, Blue and Gold Oyster, Shiitake, King Stropharia, Lions Mane, Almond Portabello, Elm Oyster, Phoenix Oyster, Nameko, Enoki, White Beech, Spawn I Want: Corcyceps Sp., Cauliflower Mushrooms, Agaricus species (Portobello), Pink Oyster, Piopinno, ...anything else you might have Primitive Skills Any information I've posted is only related to fictional purposes. I do not advocate growing any illegal mushrooms ...I DO advocate learning about Law Enforcement Against Prohibition
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goop
Registered: 07/10/08
Posts: 595
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Re: Sterilze Needle.......... [Re: RonPaulVerm]
#8688178 - 07/27/08 12:34 PM (15 years, 7 months ago) |
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there's no problem using a cigarette lighter to sterilize your syringe needle. there are no dangers in doing so.
-------------------- whats this man saying?
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psylopsycho
Motivational Speaker
Registered: 06/22/08
Posts: 503
Loc: The Tundra
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Re: Sterilze Needle.......... [Re: goop]
#8688185 - 07/27/08 12:37 PM (15 years, 7 months ago) |
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There are no dangers. Just a little cumbersome lighting a BIC while wearing gloves. You also risk ripping the glove.
Alcohol lamps are cheap, and easy to use.
Good Luck
-------------------- Someone actually said: "some 1 told me to try long brown rice and water and vermiculite and pressure cook for 1 hour i did but nothing happen that is y was wonting to no if i need spores ""
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