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InvisiblePhish_Dude
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70% ethyl alcohol adequate to sterilize glovebox?
    #8555950 - 06/23/08 08:19 PM (15 years, 8 months ago)

well?


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Offlinemyc0man
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: Phish_Dude]
    #8555965 - 06/23/08 08:24 PM (15 years, 8 months ago)

You sure it's ethyl?

Yes if so.

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InvisiblePhish_Dude
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: myc0man]
    #8555984 - 06/23/08 08:29 PM (15 years, 8 months ago)

it surely is i was under the impression that only 91% would sterilize i guess i can inoculate now


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OfflineMHbound
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: Phish_Dude]
    #8555998 - 06/23/08 08:33 PM (15 years, 8 months ago)

A glove box mainly keeps the airflow to a minimum. Sterilizing with a light clorox mix is fine.


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Offlinemyc0man
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: Phish_Dude]
    #8556023 - 06/23/08 08:36 PM (15 years, 8 months ago)

Quote:

it surely is i was under the impression that only 91% would sterilize i guess i can inoculate now



Negative :smile: 70% will sterilize surfaces

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InvisiblePhish_Dude
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: MHbound]
    #8556048 - 06/23/08 08:43 PM (15 years, 8 months ago)

Quote:

MHbound said:
A glove box mainly keeps the airflow to a minimum. Sterilizing with a light clorox mix is fine.



im almost out of alcohol what does the mixture consist of??


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OfflineRogerRabbitM
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: Phish_Dude]
    #8556106 - 06/23/08 08:56 PM (15 years, 8 months ago)

You don't sterilize gloveboxes.  Wash with soap and water and use. 

Don't spray alcohol, Lysol, or any other flammable substance and then light a flame to sterilize your tools.  More than one grower has learned that the hard way.

In addition, alcohol doesn't sterilize anything.  If you're trying to sanitize a countertop or latex gloves, etc., 70% alcohol is more effective than 91% or even 99%.  Just don't use it in your glovebox, you'll make a bomb.
RR


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Offlineusagolden
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: RogerRabbit]
    #8556140 - 06/23/08 09:01 PM (15 years, 8 months ago)

yes

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Offlinemyc0man
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: usagolden]
    #8556143 - 06/23/08 09:02 PM (15 years, 8 months ago)

Word. My bad on the terminology.

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InvisiblePhish_Dude
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: RogerRabbit]
    #8556147 - 06/23/08 09:02 PM (15 years, 8 months ago)

Quote:

RogerRabbit said:
You don't sterilize gloveboxes.  Wash with soap and water and use. 

Don't spray alcohol, Lysol, or any other flammable substance and then light a flame to sterilize your tools.  More than one grower has learned that the hard way.

In addition, alcohol doesn't sterilize anything.  If you're trying to sanitize a countertop or latex gloves, etc., 70% alcohol is more effective than 91% or even 99%.  Just don't use it in your glovebox, you'll make a bomb.
RR




wow thanks for telling me that, i feel really stupid that i didn't come to that realization.


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OfflineBrandNewbie
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: Phish_Dude]
    #8556426 - 06/23/08 10:22 PM (15 years, 8 months ago)

Quote:

Phish_Dude said:
wow thanks for telling me that, i feel really stupid that i didn't come to that realization.




At least you didn't feel as stupid as those who lost their eyebrows. :wink:


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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: RogerRabbit]
    #8557259 - 06/24/08 05:11 AM (15 years, 8 months ago)

Quote:

RogerRabbit said:
You don't sterilize gloveboxes.  Wash with soap and water and use. 

Don't spray alcohol, Lysol, or any other flammable substance and then light a flame to sterilize your tools.  More than one grower has learned that the hard way.

In addition, alcohol doesn't sterilize anything.  If you're trying to sanitize a countertop or latex gloves, etc., 70% alcohol is more effective than 91% or even 99%.  Just don't use it in your glovebox, you'll make a bomb.
RR




to sanitize a countertop or latex gloves, etc., 70% alcohol is more effective than 91% or even 99%

So are you saying 70% is better to use in this hobby than 91%?

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Offlineblackclw
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: Jrsxt]
    #8557384 - 06/24/08 07:03 AM (15 years, 8 months ago)

70% alcohol is better because it penetrates more easily into cells, so it`s more effective then any other percentage.

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Offlinerobanero
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: blackclw]
    #8557836 - 06/24/08 10:21 AM (15 years, 8 months ago)

I've read somewhere that 50% is even better? Is this true Roger?


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OfflineRoosterCogburn
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: robanero]
    #8557842 - 06/24/08 10:23 AM (15 years, 8 months ago)

The other 30% in 70% alcohol is water. It allows the alcohol to pass into the cells (as said) and do it's job.

91% and higher is good for hash amd cleaning bongs. :smile:

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Offlineblackout
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: Jrsxt]
    #8557987 - 06/24/08 11:17 AM (15 years, 8 months ago)

Quote:

Jrsxt said:
sanitize a countertop or latex gloves, etc., 70% alcohol is more effective than 91% or even 99%

So are you saying 70% is better to use in this hobby than 91%?



The reason is that 91% evaporates quickly so has little time to act on a surface. If you wanted to kill some vegetative growth then if you opened a bottle of 91% and 70%, threw it in and capped it, then it would be killed faster in the 91%. If wiping a surface the 70% will last longer so end up killing more. You could compare it to PCing for 1min at 15psi or 15 mins at 10psi

I just get 96%, I used to make it myself. So if you want 70% just dilute it down.

96% has its place too. I use it on needles between innoculations, since there is very little time to act anyway, and I want it evaporated fast. I expect it could be used in cloning too, get a stem and dip it in it, the moist tissue will probably dilute it a little around the surface.

I put all my stuff and a 96% spray in the glove box, since it is so enclosed it takes a good while to evaporate off, so will get to  work well. It escapes through the small filter patch on my box.

I put tissue in the box and spray it inside and wipe down all my equipment inside the box.

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OfflineRoosterCogburn
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: blackout]
    #8558000 - 06/24/08 11:21 AM (15 years, 8 months ago)

Holy shit, I just googled the subject to read a little more and THIS THREAD is already in the search results!!

Are the threads sent directly to google?!

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OfflineMHbound
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: RoosterCogburn]
    #8558611 - 06/24/08 02:30 PM (15 years, 8 months ago)

Google has bots that roam the internet logging changes to web pages...So yes it would see, and it would add this page...Lookup the google cloud for more information about how it works...Off topic sorry.


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OfflineRoosterCogburn
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: MHbound]
    #8559094 - 06/24/08 04:52 PM (15 years, 8 months ago)

I know that, I didn't realize that drug related message board threads get updated daily.... kinda creepy since the internetz is pretty big! :shrug:

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OfflineRogerRabbitM
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Re: 70% ethyl alcohol adequate to sterilize glovebox? [Re: RoosterCogburn]
    #8559201 - 06/24/08 05:17 PM (15 years, 8 months ago)

Google and yahoo both crawl the shroomery daily.  Yahoo  hits my own website at 1:29 pm every day right on the clock.  Any changes are updated within hours.  Google runs a bit slower at updating than Yahoo, but still puts stuff up the same day they crawl.  There's code on each page telling the bots what to look at and what to skip, such as PMs.

The evaporation rate is only part of the equation.  70% is better at attacking cells because the water in it 'tricks' the cell wall into admitting it, and then once inside, the alcohol kills the cell.  If it can't penetrate, it doesn't kill the cell.

It's the same phenomena that makes a bleach water solution more effective if you add a vinegar.  Bleach has a very high pH, so the cell wall wants to exclude it, sensing toxin.  However, the vinegar brings the pH down to a level where the cell wall opens up and allows it in, thus the bleach is able to do its job of killing the cell.
RR


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