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praga
Stranger
Registered: 04/19/08
Posts: 32
Last seen: 15 years, 5 months
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WBS growth stalls?
#8515924 - 06/12/08 03:19 PM (15 years, 9 months ago) |
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I followed Docs WBS Tek, pretty much to the T. Ive done it twice...14 quarts in total. They start out ok, but they seem to fill up with myc-piss or something and then stall out. I had to totally trash the first batch as the eventually got contaminated. Im now on the 2nd 7 quarts and its doing the same thing. Everything was looking good till about 50% colonization, I beat the jars on a tire and shook them and about a week later started noticing excessive fluid in the bottoms.
Currently, I have 5 jars left and all of them are about 100% colonized on the top half of the WBS, the bottom half looking pretty bare. Should I go ahead and try to spawn them? I dont want this to be a total loss.
I guess I bit off more than I could chew. The bulk teks didnt seem too tough but I cant get it right apparently. I started 16 BRF half-pints last night, hopefully I have better luck.
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: WBS growth stalls? [Re: praga]
#8515943 - 06/12/08 03:24 PM (15 years, 9 months ago) |
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Take the good jars that you have and shake them and wait for the bottom half to colonize before using them as spawn,if you REALLY want to spawn them now be careful to only use what you have that is colonized,any uncolonized spawn should be discarded before it becomes a vector for contamination
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praga
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Re: WBS growth stalls? [Re: Blutjager]
#8515955 - 06/12/08 03:28 PM (15 years, 9 months ago) |
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Yeah, but it simply wont colonize. It's been in the incubator for a loong damn time. The excess fluid in the jar is the issue. Should i try to draw it out with a syringe?
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: WBS growth stalls? [Re: praga]
#8515984 - 06/12/08 03:35 PM (15 years, 9 months ago) |
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I would not try drawing it out with a syringe unless you are 100 % sure of your sterile technique,anything bad you introduce into those jars now will grow like wild fire on the uncolonized grains in your jars
I can tell you though that for next time you will get ALLOT less of it if you incubate at room temp,in my opinion cubes should never be incubated unless wherever you are growing them has a freezing room temperature,ditch the incubator and I bet it improves greatly next time
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praga
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Re: WBS growth stalls? [Re: praga]
#8516025 - 06/12/08 03:44 PM (15 years, 9 months ago) |
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My incubator is set at about 81*....I figured it would help. I suppose I could turn it off.
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BrandNewbie
Captain
Registered: 05/21/08
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Re: WBS growth stalls? [Re: praga]
#8516032 - 06/12/08 03:46 PM (15 years, 9 months ago) |
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Do you have any pics?
Are you over-soaking/simmering?
Is moisture getting into the jars during the pc?
Are you adding too many cc's at inoc?
You have to determine why you have too much moisture.
One thing everyone seems to agree on is, only use wbs that is fully colonized, or it'll be a miracle if you don't get contams!
Good luck, bro!
-------------------- Question: Why do women wear make-up and perfume? Answer: Because they're ugly and they stink.
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BrandNewbie
Captain
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Re: WBS growth stalls? [Re: Blutjager]
#8516040 - 06/12/08 03:48 PM (15 years, 9 months ago) |
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Sorry Blut. I didn't know you were still talking....
Right on.
-------------------- Question: Why do women wear make-up and perfume? Answer: Because they're ugly and they stink.
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praga
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Re: WBS growth stalls? [Re: praga]
#8516085 - 06/12/08 04:03 PM (15 years, 9 months ago) |
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Some pics...
About a month ago
Today
Close up of the worst one
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praga
Stranger
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Re: WBS growth stalls? [Re: praga]
#8516104 - 06/12/08 04:07 PM (15 years, 9 months ago) |
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One of these days I'll get around to picking up that Gorton's grilled fish box. lol
So...best bet is try to draw it out with a syringe?
I must be over soaking/simmering. I am following Doc's tek though, doing exactly as it says. The second batch I thought it may be too wet, so I even left it drying a little longer than Doc specified. Maybe WBS just isn't my thing yet. Is there another bulk tek that is easier to get the moisture content right with?
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nonlinear
armour plated farmer
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Re: WBS growth stalls? [Re: praga]
#8516209 - 06/12/08 04:36 PM (15 years, 9 months ago) |
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are you sure that is myc piss? it looks totally out of control.
maybe you should sacrifice a jar and open it up for a smell. if it's good, use the colonized spawn for g2g. i think the easiest way to do that would be to transfer the spawn using a sterile spoon.
good luck man!
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praga
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Re: WBS growth stalls? [Re: nonlinear]
#8516245 - 06/12/08 04:50 PM (15 years, 9 months ago) |
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Quote:
nonlinear said: are you sure that is myc piss? it looks totally out of control.
maybe you should sacrifice a jar and open it up for a smell. if it's good, use the colonized spawn for g2g. i think the easiest way to do that would be to transfer the spawn using a sterile spoon.
good luck man!
Thanks man. That sounds like a good plan. Can you recommend a good tek for me, considering the moisture problems I keep having?
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BrandNewbie
Captain
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Re: WBS growth stalls? [Re: praga]
#8516259 - 06/12/08 04:52 PM (15 years, 9 months ago) |
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Well, all of your grains absorb relatively little moisture due to their construction, but that looks like my grandmother's chihuahua piddled in your jars!
Maybe you should try corn if these don't work.
Looks like you shouldn't have been heating them though.
Are they even wetter in the older pic?
1)If they still smelled good, I would accept a lesser yield and case the good myc. Make sure that any seeds that lack colonization get tossed out. Every one.
2)If you think (know) that you can rid the jars of the excess moisture without causing a contamination and they don't smell contamed, I'd do what Blutjager says, (shake and wait).
I think those are your only options as the bottom of your jars are under water.
-------------------- Question: Why do women wear make-up and perfume? Answer: Because they're ugly and they stink.
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BrandNewbie
Captain
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Re: WBS growth stalls? [Re: nonlinear]
#8516272 - 06/12/08 04:57 PM (15 years, 9 months ago) |
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Another option. Sounds like it would be fun to try as well. Great idea!
Oh, good pictures by the way!!!
-------------------- Question: Why do women wear make-up and perfume? Answer: Because they're ugly and they stink.
Edited by BrandNewbie (06/12/08 04:58 PM)
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littlediddy2001
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Re: WBS growth stalls? [Re: praga]
#8516273 - 06/12/08 04:57 PM (15 years, 9 months ago) |
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Those look nasty......
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praga
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Quote:
BrandNewbie said: Well, all of your grains absorb relatively little moisture due to their construction, but that looks like my grandmother's chihuahua piddled in your jars!
Are they even wetter in the older pic?
That's whats strange. They looked fine till I shook them. Maybe I shouldn't shake them so hard....dunno. I do beat the shit out of them on a tire though.
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BrandNewbie
Captain
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Re: WBS growth stalls? [Re: praga]
#8516313 - 06/12/08 05:05 PM (15 years, 9 months ago) |
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Maybe you don't know your own strength and you frappe'd them on the tire. Hell, if they smell good start selling syringes!
Good luck bro.
-------------------- Question: Why do women wear make-up and perfume? Answer: Because they're ugly and they stink.
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Blutjager
Inhuman
Registered: 06/11/06
Posts: 9,220
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Re: WBS growth stalls? [Re: praga]
#8518837 - 06/13/08 08:47 AM (15 years, 9 months ago) |
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Quote:
praga said: Some pics...
About a month ago
Today
Close up of the worst one
Bad bacterial contamination,Bacillus I'm fairly sure
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Neobean
Adept Mycologist
Registered: 10/07/01
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Re: WBS growth stalls? [Re: Blutjager]
#8518854 - 06/13/08 08:51 AM (15 years, 9 months ago) |
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Thats fugly...
-------------------- If y0u want s0meting gr0wn right, y0u g0tta gr0w it y0urself!!!
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xeallos
Sore wa himitsudesu!
Registered: 09/13/06
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Re: WBS growth stalls? [Re: Neobean]
#8518915 - 06/13/08 09:14 AM (15 years, 9 months ago) |
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You got bacillus like a mofo, and need to toss those bitches. Don't even try to salvage the colonized portion, at this point it is soaked with bacillus. Bacillus looks substantially different than the metabolites produced by mycelium. You have so much bacillus it is easy to explain, it is so thick and milky and discolored - off white/beige slime, not exactly "yellow". The metabolites produced by the mycelium are generally a relatively clear liquid discharge that is intense in color hence the misapplication of the term "piss".
Your moisture content may have been off, your incubation temperature certainly was. Keep grain jars at 65-70F Max, they can throw off 5-10 when the colonization moves along significantly. Also, grain is very sensitive to temperature swings so anything out of the range of +/- 5 degrees daily tends to fuck with the moisture content and be all around bad news due to forced air exchanges.
Using a fridge with a heating mat in there looks like a fire hazard to me, build a TiT incubator with an aquarium water heater if you are seriously in need of an incubation unit.
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Drew Tyler
Look into the sky
Registered: 04/21/08
Posts: 323
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Re: WBS growth stalls? [Re: xeallos]
#8519101 - 06/13/08 10:34 AM (15 years, 9 months ago) |
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You're doing something wrong...
stop using coffee filters as a filter and review your sterile procedures... could have also been contaminated syrince/lc/spawn
-------------------- I'm a terrible, no good, very bad person.
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