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Offlinenonlinear
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Registered: 01/03/08
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agar media from dextrose corn sugar?
    #8413226 - 05/17/08 02:56 PM (15 years, 10 months ago)

hey, i've got a few buckets of dextrose corn sugar laying around (use it for brewing beer). does anyone know if i can use this to prepare agar media? I've seen stuff for potato dextrose, but not corn. I imagine it would work, but looking for some recipes or teks or some shieat.


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OfflineP.Menace
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Re: agar media from dextrose corn sugar? [Re: nonlinear]
    #8413246 - 05/17/08 03:00 PM (15 years, 10 months ago)

It should work for you... its a nutrient that is already used in mycology (WBS, Cracked corn) so i dont see why it would not work for you;)


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roby000 said: thats true a shotgun is almost like a college degree in a sense that if you show it to the right person at the right time you could make a lot of money.

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Offlinenonlinear
armour plated farmer
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Re: agar media from dextrose corn sugar? [Re: P.Menace]
    #8413293 - 05/17/08 03:08 PM (15 years, 10 months ago)

i've always worked with powder or pre-poured plates, but never tried to make it myself. anyhow i'm at home and the agar powder is at work, so thinking about running to the asian mall and doing things the old-fashioined way.

I helped my friend make potato infusion for agar once, but it didn't work very well for him. he didn't add and sugar tho.

i'm just wondering if I need potato infusion, or if i can just use agar+dextrose.

i guess i could also try the search function LOL

oh yea, also all i have here is a ghetto dimebag scale, so my masses will be off anyhow.


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OfflineHybridprX
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Re: agar media from dextrose corn sugar? [Re: P.Menace]
    #8413295 - 05/17/08 03:08 PM (15 years, 10 months ago)



Mex spores on dextrose agar.

The mixture is the same as a LC, 1tbs per every 500ml of water, if using less adjust the spoonful accordingly.


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Offlinenonlinear
armour plated farmer
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Re: agar media from dextrose corn sugar? [Re: HybridprX]
    #8413408 - 05/17/08 03:32 PM (15 years, 10 months ago)

HybridprX, how much agar should i add to that? been looking with the search function but i can't seem to find and recipes on here. i guess next i'll try the cultivation archive

anyhow, this is on wikipedia, what do you guys think:

Potato infusion is made by boiling 200g of sliced (washed but unpeeled) potatoes in water for 30 minutes and then decanting or straining off the broth. Dextrose (corn sugar) is then added (20 g/L) and the medium is sterilized by autoclaving

but again, it doesn't say how much agar to add


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OfflineHybridprX
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Re: agar media from dextrose corn sugar? [Re: nonlinear]
    #8413451 - 05/17/08 03:46 PM (15 years, 10 months ago)

I just put my dextrose/water/agar into a pot and boil it until most the agar dissolves then I add abit more once its been poored into the beer bottle/flask.


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Invisiblewisp

Registered: 04/13/08
Posts: 5,304
Re: agar media from dextrose corn sugar? [Re: HybridprX]
    #8413560 - 05/17/08 04:16 PM (15 years, 10 months ago)

http://www.shroomery.org/9393/FastFreds-Media-Cookbook

every agar recipe you'll ever need

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