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OfflineDeadCheezy
PsychedelicSpace Cadet


Registered: 04/07/08
Posts: 40
Last seen: 15 years, 7 months
G2G from PF Jars to Bulk
    #8345889 - 04/30/08 12:53 PM (15 years, 10 months ago)

Ive spent quite a bit of time reading teks and the message boards looking for the best methods for a g2g spawn to a bulk substrate from PF jars. I was wondering if anyone has a favorite tek that they have found to be more successful than others?

I have 2 fully colonized 1/2 pint, BRF/verm pf jars ready to go. If I need more, I will have another fully colonized jar within the week. I was thinking about spawning to rye berries (they seem pretty easy to find). Thoughts on spawning to rye??

I do not have a PC but I could probably manage to buy one. If this can be done EFFICIENTLY without one I would prefer that.

Im going to need all of the input that you guys can give! I just bought a new camera so Ill post pictures every step of the way! Id love some advice from a voice of experience!

Thanks very much,
DC


--------------------
-DC-

"Do you do drugs Danny?"
"Everyday."
"Good."

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Offlineamanitavirosa
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Re: G2G from PF Jars to Bulk [Re: DeadCheezy]
    #8345898 - 04/30/08 12:57 PM (15 years, 10 months ago)

If you want to move up to grain spawns, I would highly recommend a pressure cooker. You can try your hand at fractional sterilization but it takes a lot of time and is not 100%

Additionally, doing a grain-to-grain requires a lot more cleanliness. Ideally, you would do the transfers in a decent still-air box or a glove box. This greatly reduces your chances of contamination.


--------------------
-AV-

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OfflineDeadCheezy
PsychedelicSpace Cadet


Registered: 04/07/08
Posts: 40
Last seen: 15 years, 7 months
Re: G2G from PF Jars to Bulk [Re: amanitavirosa]
    #8345932 - 04/30/08 01:08 PM (15 years, 10 months ago)

Thanks for the response!

I will get a pressure cooker. All of the literature says that is by far the best way to sterilize. I also plan on building a glove or still-air box when the time comes.

How many quart jars should I buy? What kind of PC should I get?


--------------------
-DC-

"Do you do drugs Danny?"
"Everyday."
"Good."

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Invisibleyasi
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Re: G2G from PF Jars to Bulk [Re: DeadCheezy]
    #8345938 - 04/30/08 01:14 PM (15 years, 10 months ago)

how much are you planning on producing?


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Counting Bodies like Sheep...

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Offlineamanitavirosa
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Re: G2G from PF Jars to Bulk [Re: DeadCheezy]
    #8345941 - 04/30/08 01:15 PM (15 years, 10 months ago)

Those are kind of vague questions with a myriad of possibilities.

I would buy a 12-pack of quart jars and whatever PC you can afford. I would personally try to find a used PC to save some $$ but a brand new one would be just dandy too.


--------------------
-AV-

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InvisibleTomandjerry58
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Registered: 01/27/03
Posts: 5,212
Re: G2G from PF Jars to Bulk [Re: yasi]
    #8345942 - 04/30/08 01:16 PM (15 years, 10 months ago)

get a 915 all american... she's great... two cases of quart jars(24)

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Invisibleyasi
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Re: G2G from PF Jars to Bulk [Re: Tomandjerry58]
    #8345980 - 04/30/08 01:27 PM (15 years, 10 months ago)

got my presto 23 quart
works great.

I guess it's just personal preference.


--------------------
Counting Bodies like Sheep...

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OfflineTyz
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Re: G2G from PF Jars to Bulk [Re: DeadCheezy]
    #8345985 - 04/30/08 01:28 PM (15 years, 10 months ago)

the pressure cooker you want to buy should hold 15 psi look on the box for that. walmart has a 6 qt. p.cooker for 28$ that hold 15 psi where i'm at. but it wont fit quart jars, u must use pint jars with it. with 2 pf jars, one case(12) of pint jars should not be a problem. but if i were you i'd fruit the pf jars. make a couple spore prints with your fruits and innoc. a master grain jar(s), then when it(they) colonize. you have say 12 jars fully colonized grain jars. you can transfer 1/10th of each jar to another sterile un-innoc. jar of seed. or use 2 of the 12 as a master. and the other ten u spawn to bulk. and use the master to knock up 20 more jars.

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InvisibleTomandjerry58
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Registered: 01/27/03
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Re: G2G from PF Jars to Bulk [Re: yasi]
    #8345986 - 04/30/08 01:28 PM (15 years, 10 months ago)

as long as it holds atleast seven you should be good for home cultivation

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OfflineDeadCheezy
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Last seen: 15 years, 7 months
Re: G2G from PF Jars to Bulk [Re: amanitavirosa]
    #8346051 - 04/30/08 01:43 PM (15 years, 10 months ago)

Right on. I'll try to be more specific. Apologies.

I can't find the exact model of Sterilite tub I am using on the internet, so my best guess is that its about an 80 quart tub. Dims: 32"x12"x12". My goal is to produce the highest yield possible with this size of tub.

Is two 1/2 pint PF jars enough for this size of project?

I guess the only stipulations pertaining choosing the PC is that it is large enough to fit quart sized jars? EDIT: Sorry didnt see the responses!!

AV, I'm going to attempt to build a glove box similar to yours. Would it be a great disadvantage to not attach the fans and HEPA filter?

Thanks!!


--------------------
-DC-

"Do you do drugs Danny?"
"Everyday."
"Good."

Edited by DeadCheezy (04/30/08 01:44 PM)

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Offlineamanitavirosa
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Re: G2G from PF Jars to Bulk [Re: DeadCheezy]
    #8346060 - 04/30/08 01:51 PM (15 years, 10 months ago)

You should really start with more colonized grain so that the mycelia have a chance to take a strong hold of the substrate before any contaminants can. I would spawn those jars to grain jars to up your inoculum volume.

And in regards to a glove box - no, the fans and HEPA filter are not necessary, they are merely a bonus. I like to work in a positively pressurized environment for any sterile work - its what I am used to. All you need is a "still air" box really.


--------------------
-AV-

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OfflineDeadCheezy
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Registered: 04/07/08
Posts: 40
Last seen: 15 years, 7 months
Re: G2G from PF Jars to Bulk [Re: amanitavirosa]
    #8346074 - 04/30/08 01:58 PM (15 years, 10 months ago)

Okay sounds good.

Regarding the spawn to more grain jars what procedure do you recommend? Is there any specific reading I can do to better understand this process?

I was under the impression that I could take these two pf jars, shred the cakes, or scoop them using 1-2 tablespoons of colonized cake, into sterilized rye berry jars. Allow those to colonize then spawn those jars to the bulk. Am I even close?


--------------------
-DC-

"Do you do drugs Danny?"
"Everyday."
"Good."

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Offlineamanitavirosa
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Registered: 04/07/08
Posts: 430
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Re: G2G from PF Jars to Bulk [Re: DeadCheezy]
    #8346100 - 04/30/08 02:07 PM (15 years, 10 months ago)

You are exactly right. Do pretty much exactly as you mentioned (but in a glove box) and you will have done a grain-to-grain transfer. This will be a key component of your future grows.


--------------------
-AV-

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OfflineDeadCheezy
PsychedelicSpace Cadet


Registered: 04/07/08
Posts: 40
Last seen: 15 years, 7 months
Re: G2G from PF Jars to Bulk [Re: amanitavirosa]
    #8346168 - 04/30/08 02:24 PM (15 years, 10 months ago)

Awesome!

So I'll get a PC within the next few days as well as some quart sized jars.

How about a grain suggestion? Should I go with the rye berries? I haven't looked around at all so if there is something that would help speed colonization or provide more nutes I'll look around for anything. I live in a place where feed stores, walmarts, and flower nurseries are readily available.


--------------------
-DC-

"Do you do drugs Danny?"
"Everyday."
"Good."

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InvisibleTomandjerry58
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Registered: 01/27/03
Posts: 5,212
Re: G2G from PF Jars to Bulk [Re: DeadCheezy]
    #8346212 - 04/30/08 02:36 PM (15 years, 10 months ago)

you know you can inoculate grain with spore syringes... right? just wanted to make sure you knew that... like aminita advised you already... grain is the ideal thing to use because of the multiple inoculation points

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OfflineDeadCheezy
PsychedelicSpace Cadet


Registered: 04/07/08
Posts: 40
Last seen: 15 years, 7 months
Re: G2G from PF Jars to Bulk [Re: Tomandjerry58]
    #8346242 - 04/30/08 02:44 PM (15 years, 10 months ago)

I did know that. After reading a some accounts of the g2g method it sounds like the mycelium colonizes quicker than just straight up spore syringe inoculation. In fact I read somewhere that if you use like a cheese grater to grate the BRF cakes you can get full colonization of a quart jar in less than 10 days...

My first grow using the 50/50+ casing tek is fruiting now so I could make a print/syringe with some of these...

Would inoculation with a syringe be easier or have less chance of failure?


--------------------
-DC-

"Do you do drugs Danny?"
"Everyday."
"Good."

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Invisiblesmokedout420
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Re: G2G from PF Jars to Bulk [Re: DeadCheezy]
    #8346351 - 04/30/08 03:35 PM (15 years, 10 months ago)

presto 23 quart has a gauge and works great i got 2 of em

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InvisibleTomandjerry58
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Re: G2G from PF Jars to Bulk [Re: smokedout420]
    #8346447 - 04/30/08 04:16 PM (15 years, 10 months ago)

i would say so... using a cheese grater would seem like a high risk of contamination.... use a glove box set up. get a spoon or something and a half full glass of h202 (hydrogen peroxide) put the spoon in the glass.... then just scoop one or two pieces off and put it in the jar quickly and close lid..... just continue to do this until you run out of jars or cakes which ever comes first..... put spoon back into h202 for every jar change.           
good luck man:mushroom2:

once the mycelium takes hold i would give it a shake

(about 4 days after)

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OfflineDeadCheezy
PsychedelicSpace Cadet


Registered: 04/07/08
Posts: 40
Last seen: 15 years, 7 months
Re: G2G from PF Jars to Bulk [Re: Tomandjerry58]
    #8348156 - 05/01/08 12:22 AM (15 years, 10 months ago)

Late night idea...

I have a 6x8" casing that just provided a much less than desirable first flush... I have a second casing in the FC, so I'm not particularly worried about the fate of the first casing. Because the mycelium already has a strong hold on the substrate and has colonized much of the casing layer, could I not treat the casing as if it was say, 3 quart sized jars of colonized substrate? Could I spawn the casing itself into a bulk substrate?

I've been laying in bed just thinking about how obsessed I have become with this. So I really sorry if this question is completely ridiculous...


--------------------
-DC-

"Do you do drugs Danny?"
"Everyday."
"Good."

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