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Offlineshoeareyou
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Registered: 01/07/07
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Water on the AGAR... colonies wash away
    #8161784 - 03/18/08 11:09 AM (16 years, 13 days ago)

Hi there, Day 2 of incubation and im a little concerned that my dextrose agar slopes petri dishes have too much water on the surface. the agar was preforumulated but im pretty sure its condensation or spore solution. Colonization has begun, tiny colonies are present but when i moved one of the slopes they washed off. should this be a problem, or what?

Im after isolation of rhizomorphic strains, so how is this possible when all of the colonies are sloshing about?

(Or, in this case, do you think it was more likely to be a bacterial colony, since it w

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OfflineanarchOi
Ellenalien is fat.
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Registered: 08/06/07
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Re: Water on the AGAR... colonies wash away [Re: shoeareyou]
    #8161802 - 03/18/08 11:14 AM (16 years, 13 days ago)

you deffinitely shouldn't have ANYTHING sloshing in your agar...

sounds alot like you have trich to me
if after day 2, you have a bunch of little tiny colonies......

that's not good.


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OfflineCiv
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Re: Water on the AGAR... colonies wash away [Re: anarchOi]
    #8161856 - 03/18/08 11:27 AM (16 years, 13 days ago)

You should not have any condensation in your agar. Are you HEATING them or something?

are you for sure its myc growing and not something else? you used to much solution if you have enough to 'slosh' around and the growth moves about.

HEAT will cause your medium to seperate, and you'll see water.

Please post up some pics, it will help if you really need it.


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"...Gal's seem to hate the thought of blending chicken shit in a blender.
So, wash it well afterwards & DON'T tell them..."  -Agar

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Offlineshoeareyou
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Re: Water on the AGAR... colonies wash away [Re: Civ]
    #8162411 - 03/18/08 02:04 PM (16 years, 13 days ago)

nope, im not doing anything crazy like heating them. (Except incubation at 79F - 80F [27.2*C])

these were dextrose agar slopes which I purchased from an online vendor (applied microscience) prepoured and presterilized. I only opened them once inside my glovebox, and sprayed with bleach. I also flamed the opening, aswell as the spore syringe, etc.

sterile as much as possible.

P.S It isn't REALLY day 2, its actually more like day 4 - but Im calling it day 2 since I accidentally left the incubator turned off (tripped over the cable and pulled the incubator plug out in the mad rush to get a soundsystem organised at the weekend.) then, no incubation while partying.

I was quite dissapointed when I got home.

Its definately not trych, the colonies are very very light yellow/white spots, about < 1mm in diameter, round.

Bacteria? I've never seen mycelium at such infantile stages before, so I don't know.

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OfflineanarchOi
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Re: Water on the AGAR... colonies wash away [Re: shoeareyou]
    #8162528 - 03/18/08 02:43 PM (16 years, 13 days ago)

sounds exactly like immature trich to me

wanna bet on the green? :-P


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