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Offlinenobodyathome
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Registered: 02/25/08
Posts: 2
Last seen: 15 years, 11 months
Grow bag time table
    #8148997 - 03/15/08 10:58 AM (16 years, 17 days ago)

I got bags from The Mad Season, and spores from Ralph, but something dosent seem right....


I took 3 of the bags and injected aprox 3.5ml of Mexi Cube into each of them.

The problem (question?) is that it was 17 days ago, and I have yet to see any growth. No fuzzys at all, not even contamination.

They have been kept at 75F in a dark closet.

Any thoughts?

Edited by nobodyathome (03/20/08 11:15 AM)

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Offlineitsallnines
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Registered: 04/17/07
Posts: 385
Last seen: 10 years, 5 months
Re: Grow bag time eable [Re: nobodyathome]
    #8149010 - 03/15/08 11:04 AM (16 years, 17 days ago)

i have a bag from everything mushrooms that i inoculated with oyster liquid culture and it took at least two weeks before i saw any growth. when it started, however, it really took off.


--------------------
by their fruits ye shall know them.

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Offlinedirtworshipper
Sitting in the heart cave
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Registered: 05/12/07
Posts: 2,060
Loc: at The Guru's lotus feet
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Re: Grow bag time eable [Re: nobodyathome]
    #8149099 - 03/15/08 11:36 AM (16 years, 17 days ago)

You should have started the bags with Liquid Culture.
Multispore inoculations will take a lot longer.


--------------------

“You've got as many lives as you like, and more, even ones you don't want.” - George Harrison

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Offlinenobodyathome
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Registered: 02/25/08
Posts: 2
Last seen: 15 years, 11 months
Re: Grow bag time eable [Re: dirtworshipper]
    #8171387 - 03/20/08 11:15 AM (16 years, 12 days ago)

Now I'm starting to get concerned...

It has been 22 days, and I have no growth at all.

Here is what I did..

I took the syringe, wiped the needle with alcohol, wiped the injector site on the bag with alcohol, let them air dry, then injected 3 bags with the one syringe (~3.5ml per bag). They have been in a dark closet between 70°-75° the entire time.

Could I possibly have done something wrong?

I have more bags, and different strains, but I dont want to try again till I find out whats wrong.

Ralph is one of the most respected people around here, so I really think I am the one messing up.

The PF tek and the grow bag instructions dont make any mention of a liquid culture, so just injecting should work, no?


Please help.

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Offlinedirtworshipper
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Registered: 05/12/07
Posts: 2,060
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Re: Grow bag time eable [Re: nobodyathome]
    #8171451 - 03/20/08 11:28 AM (16 years, 12 days ago)

Well, first off, when injecting spores into a grain bag, you can expect it to take a very long time (if it colonizes at all). I've always heard that liquid culture is the way to go with grain bags. Liquid Culture Basics

Secondly, you want colonization temperatures to be a bit higher. Try to get it around 78-80degrees.

Make some liquid culture and THEN inoculate your bags with it
:smileyfrog:

good luck


--------------------

“You've got as many lives as you like, and more, even ones you don't want.” - George Harrison

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