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Justice_Fish
Fustice_Jish
Registered: 01/06/07
Posts: 2,652
Loc: CebèuQ
Last seen: 7 years, 5 months
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Worst inoculation. ever.
#7977999 - 02/04/08 03:06 PM (16 years, 1 month ago) |
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And I did it.
I'll get no mycelium or a lot of contamination.
I had problems with the syringe. When I was sterilizing it, spores were still in the needle. Why?
I had problems while innoculating. I made big holes because I couldn't go very far.
Well fuck it. We'll see, and still, fuck it.
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xeallos
Sore wa himitsudesu!
Registered: 09/13/06
Posts: 678
Loc: The Woods of a Thousand Y...
Last seen: 11 years, 3 months
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Re: Worst inoculation. ever. [Re: Justice_Fish]
#7978010 - 02/04/08 03:08 PM (16 years, 1 month ago) |
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shroober
Myco Junkie
Registered: 01/02/08
Posts: 879
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Re: Worst inoculation. ever. [Re: xeallos]
#7978130 - 02/04/08 03:35 PM (16 years, 1 month ago) |
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my worst experience was when i had a 50 ml syringe full of healthy, thick, myc water bust open all over 12 quart jars of rye, that sucked
surprisingly I did an impromptu G2G and didnt get a single contam
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Worst inoculation. ever. [Re: shroober]
#7979472 - 02/04/08 07:36 PM (16 years, 1 month ago) |
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you had spores stuck in the needle while it was being sterilized??? Why is this a concern??? Please tell us in english what happened and what the possible problem is
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you. My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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enlightened seed
Utopia is a state of mind
Registered: 05/04/07
Posts: 2,117
Loc: amongst civilization
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Re: Worst inoculation. ever. [Re: Justice_Fish]
#7982637 - 02/05/08 01:11 PM (16 years, 1 month ago) |
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Quote:
Justice_Fish said: And I did it.
I'll get no mycelium or a lot of contamination.
I had problems with the syringe. When I was sterilizing it, spores were still in the needle. Why?
I had problems while innoculating. I made big holes because I couldn't go very far.
Well fuck it. We'll see, and still, fuck it.
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shevanel
Gone til November
Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 1 month
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I don't understand why you're mad?
Spores in the needle while sterilizing?
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Justice_Fish
Fustice_Jish
Registered: 01/06/07
Posts: 2,652
Loc: CebèuQ
Last seen: 7 years, 5 months
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Re: Worst inoculation. ever. [Re: shevanel]
#7982916 - 02/05/08 02:37 PM (16 years, 1 month ago) |
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Well, is this normal? I had the syringe in my hands. I did not start innoculating yet. I put the needle over the flame, and see bubbles blowing up, and I heard a little hissing sound. I don't if this is normal. I never read something about that.
Anyway, the other thing; I used 400ml jars. I don't know if the spores made completly their way down to the bottom.
Everything went smoothly until inoculation.
I hope my sterilization was fine enough, but I think contam is on the way.
And sorry for my english, it's my 3rd language.
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Worst inoculation. ever. [Re: Justice_Fish]
#7982973 - 02/05/08 02:58 PM (16 years, 1 month ago) |
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why would you think that spores made all that noise?? What happens to water when it boils??? It expands I think at a 800:1. I think this is what I recall from geology. I could be wrong.
Anyways? it is normal to have some water/spores in the needle. It is normal for it to hiss.
Even if i didn't know this already i would not have been worried. There is a thought process involved with having a human brain and the smarter ones among us process this stuff and figure it out on our own.
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you. My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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Justice_Fish
Fustice_Jish
Registered: 01/06/07
Posts: 2,652
Loc: CebèuQ
Last seen: 7 years, 5 months
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Re: Worst inoculation. ever. [Re: tahoe]
#7984126 - 02/05/08 07:37 PM (16 years, 1 month ago) |
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Quote:
tahoe said: Even if i didn't know this already i would not have been worried. There is a thought process involved with having a human brain and the smarter ones among us process this stuff and figure it out on our own.
Thanks for the insult.
just joking
I just wanted to know if it was normal for it to hiss. I never really messed up with needles in my life, and it was the first time I had one in my hands (hence the improper noobish inoculation I have done).
What I really wanted ot know, is if the loss of spores was a major one. Looks like it's not. I let cool the needle for 3-4 seconds then I inoculated, supposing the heat would not destroy any spores. Am I correct? Probably.
Well thanks for those who tried to understand my english. We'll see around the 14th if contam killed my project or not
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arainbow
Hippy
Registered: 02/04/08
Posts: 691
Loc: Palnet Earth
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Re: Worst inoculation. ever. [Re: Justice_Fish]
#7984496 - 02/05/08 08:50 PM (16 years, 1 month ago) |
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that's nothing on my first attempt I opened the spore print and touched it with dirty hands as soon as I got the kit home . all I ended up with was YEAST!!!
-------------------- There is more joy in heaven over one of us perfected, than over ninety-nine naturally evolved angels.
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Cubie
Moderator
Registered: 01/11/08
Posts: 8,840
Loc: Down the rabbit hole...
Last seen: 11 years, 10 months
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Re: Worst inoculation. ever. [Re: arainbow]
#7984550 - 02/05/08 09:00 PM (16 years, 1 month ago) |
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My first time I pulled the syringe out of the bag and couldn't find the hole to tape it. Lol I ended up putting a 4 by 4 inch square of tape on it.
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Worst inoculation. ever. [Re: Justice_Fish]
#7984674 - 02/05/08 09:25 PM (16 years, 1 month ago) |
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Quote:
Justice_Fish said:
I just wanted to know if it was normal for it to hiss. I never really messed up with needles in my life, :
have you ever poured water onto a fire to put it out, have you ever pissed on a camp fire, have you ever poured water into a dry/hot pan?
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you. My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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HybridprX
Biodegrader of coir
Registered: 01/29/08
Posts: 2,588
Loc: Canada
Last seen: 6 years, 6 months
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Re: Worst inoculation. ever. [Re: tahoe]
#7984686 - 02/05/08 09:27 PM (16 years, 1 month ago) |
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First time ever... I inoculated and then boiled the pf cake...
ha-ha-ha
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tahoe
Noob Slayer
Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
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Re: Worst inoculation. ever. [Re: HybridprX]
#7984698 - 02/05/08 09:30 PM (16 years, 1 month ago) |
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Quote:
HybridprX said: First time ever... I inoculated and then boiled the pf cake...
ha-ha-ha
this I do not doubt. This is the same guy that pm's me trying to match wits
-------------------- Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you. My Legacy https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987 Teh=The I need to proofread
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