Home | Community | Message Board

World Seed Supply
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineJustice_Fish
Fustice_Jish
Male


Registered: 01/06/07
Posts: 2,652
Loc: CebèuQ
Last seen: 7 years, 5 months
Worst inoculation. ever.
    #7977999 - 02/04/08 03:06 PM (16 years, 1 month ago)

And I did it. :sad:

I'll get no mycelium or a lot of contamination.

I had problems with the syringe. When I was sterilizing it, spores were still in the needle. Why?

I had problems while innoculating. I made big holes because I couldn't go very far.

Well fuck it. We'll see, and still, fuck it.

Extras: Filter Print Post Top
Offlinexeallos
Sore wa himitsudesu!
 User Gallery


Folding@home Statistics
Registered: 09/13/06
Posts: 678
Loc: The Woods of a Thousand Y...
Last seen: 11 years, 3 months
Re: Worst inoculation. ever. [Re: Justice_Fish]
    #7978010 - 02/04/08 03:08 PM (16 years, 1 month ago)

:shrug:

Extras: Filter Print Post Top
Invisibleshroober
Myco Junkie

Registered: 01/02/08
Posts: 879
Re: Worst inoculation. ever. [Re: xeallos]
    #7978130 - 02/04/08 03:35 PM (16 years, 1 month ago)

my worst experience was when i had a 50 ml syringe full of healthy, thick, myc water bust open all over 12 quart jars of rye, that sucked

surprisingly I did an impromptu G2G and didnt get a single contam

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Worst inoculation. ever. [Re: shroober]
    #7979472 - 02/04/08 07:36 PM (16 years, 1 month ago)

you had spores stuck in the needle while it was being sterilized???
Why is this a concern???
Please tell us in english what happened and what the possible problem is


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
Invisibleenlightened seed
Utopia is a state of mind
Male User Gallery


Registered: 05/04/07
Posts: 2,117
Loc: amongst civilization
Re: Worst inoculation. ever. [Re: Justice_Fish]
    #7982637 - 02/05/08 01:11 PM (16 years, 1 month ago)

Quote:

Justice_Fish said:
And I did it. :sad:

I'll get no mycelium or a lot of contamination.

I had problems with the syringe. When I was sterilizing it, spores were still in the needle. Why?

I had problems while innoculating. I made big holes because I couldn't go very far.

Well fuck it. We'll see, and still, fuck it.




:meff:

Extras: Filter Print Post Top
Offlineshevanel
Gone til November
 User Gallery


Registered: 01/27/08
Posts: 1,517
Last seen: 2 years, 1 month
Re: Worst inoculation. ever. [Re: enlightened seed]
    #7982658 - 02/05/08 01:17 PM (16 years, 1 month ago)

I don't understand why you're mad?

Spores in the needle while sterilizing?

Extras: Filter Print Post Top
OfflineJustice_Fish
Fustice_Jish
Male


Registered: 01/06/07
Posts: 2,652
Loc: CebèuQ
Last seen: 7 years, 5 months
Re: Worst inoculation. ever. [Re: shevanel]
    #7982916 - 02/05/08 02:37 PM (16 years, 1 month ago)

Well, is this normal? I had the syringe in my hands. I did not start innoculating yet.
I put the needle over the flame, and see bubbles blowing up, and I heard a little hissing sound.
I don't if this is normal. I never read something about that.

Anyway, the other thing; I used 400ml jars. I don't know if the spores made completly their way down to the bottom.

Everything went smoothly until inoculation.

I hope my sterilization was fine enough, but I think contam is on the way.


And sorry for my english, it's my 3rd language.

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Worst inoculation. ever. [Re: Justice_Fish]
    #7982973 - 02/05/08 02:58 PM (16 years, 1 month ago)

why would you think that spores made all that noise?? What happens to water when it boils???
It expands I think at a 800:1. I think this is what I recall from geology. I could be wrong.

Anyways? it is normal to have some water/spores in the needle. It is normal for it to hiss.

Even if i didn't know this already i would not have been worried. There is a thought process involved with having a human brain and the smarter ones among us process this stuff and figure it out on our own.


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
OfflineJustice_Fish
Fustice_Jish
Male


Registered: 01/06/07
Posts: 2,652
Loc: CebèuQ
Last seen: 7 years, 5 months
Re: Worst inoculation. ever. [Re: tahoe]
    #7984126 - 02/05/08 07:37 PM (16 years, 1 month ago)

Quote:

tahoe said:
Even if i didn't know this already i would not have been worried. There is a thought process involved with having a human brain and the smarter ones among us process this stuff and figure it out on our own.




Thanks for the insult.:cheers:

just joking

I just wanted to know if it was normal for it to hiss. I never really messed up with needles in my life, and it was the first time I had one in my hands (hence the improper noobish inoculation I have done).

What I really wanted ot know, is if the loss of spores was a major one. Looks like it's not. I let cool the needle for 3-4 seconds then I inoculated, supposing the heat would not destroy any spores. Am I correct? Probably.

Well thanks for those who tried to understand my english. We'll see around the 14th if contam killed my project or not :grin:

Extras: Filter Print Post Top
Invisiblearainbow
Hippy

Registered: 02/04/08
Posts: 691
Loc: Palnet Earth
Re: Worst inoculation. ever. [Re: Justice_Fish]
    #7984496 - 02/05/08 08:50 PM (16 years, 1 month ago)

that's nothing
on my first attempt I opened the spore print and touched it with dirty hands as soon as I got the kit home .
all I ended up with was YEAST!!!


--------------------
There is more joy in heaven over one of us perfected,
  than over ninety-nine naturally evolved angels.

Extras: Filter Print Post Top
OfflineCubie
Moderator
Male


Folding@home Statistics
Registered: 01/11/08
Posts: 8,840
Loc: Down the rabbit hole...
Last seen: 11 years, 10 months
Re: Worst inoculation. ever. [Re: arainbow]
    #7984550 - 02/05/08 09:00 PM (16 years, 1 month ago)

My first time I pulled the syringe out of the bag and couldn't find the hole to tape it. Lol
I ended up putting a 4 by 4 inch square of tape on it.

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Worst inoculation. ever. [Re: Justice_Fish]
    #7984674 - 02/05/08 09:25 PM (16 years, 1 month ago)

Quote:

Justice_Fish said:


I just wanted to know if it was normal for it to hiss. I never really messed up with needles in my life, :




have you ever poured water onto a fire to put it out, have you ever pissed on a camp fire, have you ever poured water into a dry/hot pan?


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
OfflineHybridprX
Biodegrader of coir
Male User Gallery


Registered: 01/29/08
Posts: 2,588
Loc: Canada Flag
Last seen: 6 years, 6 months
Re: Worst inoculation. ever. [Re: tahoe]
    #7984686 - 02/05/08 09:27 PM (16 years, 1 month ago)

First time ever... I inoculated and then boiled the pf cake...

ha-ha-ha


--------------------

Extras: Filter Print Post Top
Invisibletahoe
Noob Slayer
Male User Gallery


Registered: 11/26/03
Posts: 6,274
Loc: N38.93829W119.98108
Trusted Cultivator
Re: Worst inoculation. ever. [Re: HybridprX]
    #7984698 - 02/05/08 09:30 PM (16 years, 1 month ago)

Quote:

HybridprX said:
First time ever... I inoculated and then boiled the pf cake...

ha-ha-ha



this I do not doubt. This is the same guy that pm's me trying to match wits


--------------------
Stop experimenting half way through your first grow. Grow it to maturity, watch it, learn from it. Do this a few times then experiment with different ideas and figure out what works best for you.


My Legacy
https://www.shroomery.org/forums/showflat.php/Number/22140987#22140987

Teh=The
I need to proofread

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Isolated Cubensis Liquid Culture For Sale   North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Sterilizing with Heat or Fire, versus wiping with alcohol (needle and others) Anonymous 15,262 8 01/14/05 03:56 PM
by Anonymous
* Inoculate in the middle? dumlovesyou 964 4 11/16/02 03:40 AM
by dumlovesyou
* Easy to make Inoculation hood lysergic 6,596 11 07/10/03 03:58 PM
by lysergic
* Steaming and Inoculation questions podman19 1,452 7 03/29/02 12:09 AM
by WakingUpLate
* Worst Smell Ever
( 1 2 all )
MoneyAddyct 2,068 26 10/20/04 06:53 AM
by MoneyAddyct
* Problems with needle and inoculation? Katrina 630 2 12/10/04 07:59 PM
by Prisoner#1
* inoculation tip jeff01 656 2 12/23/04 07:00 PM
by jeff01
* Scared about inoculation Arrakis 1,378 17 03/20/04 11:18 AM
by menlatin

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
763 topic views. 24 members, 130 guests and 124 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.031 seconds spending 0.01 seconds on 14 queries.